دورية أكاديمية
أعرض في EDS

Antiangiogenic effects of phospholipase A 2 Lys49 BnSP-7 from Bothrops pauloensis snake venom on endothelial cells: An in vitro and ex vivo approach.

التفاصيل البيبلوغرافية
العنوان: Antiangiogenic effects of phospholipase A 2 Lys49 BnSP-7 from Bothrops pauloensis snake venom on endothelial cells: An in vitro and ex vivo approach.
المؤلفون: Polloni L; Biotechnology Institute, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Azevedo FVPV; Biotechnology Institute, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Teixeira SC; Department of Immunology, Biomedical Sciences Institute, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Moura E; Biotechnology Institute, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Costa TR; Biotechnology Institute, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Gimenes SNC; Butantan Institute, São Paulo, SP, Brazil., Correia LIV; Biotechnology Institute, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Freitas V; Biotechnology Institute, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Yoneyama KAG; Biotechnology Institute, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Rodrigues RS; Biotechnology Institute, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Lopes DS; Multidisciplinary Institute for Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil; Institute of Health Sciences, Department of biochemistry and biophysics, Federal University of Bahia, Salvador, BA, Brazil. Electronic address: lsdaiana@ufba.br., Rodrigues VM; Biotechnology Institute, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil. Electronic address: veridiana@ufu.br.
المصدر: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2021 Apr; Vol. 72, pp. 105099. Date of Electronic Publication: 2021 Jan 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 8712158 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3177 (Electronic) Linking ISSN: 08872333 NLM ISO Abbreviation: Toxicol In Vitro Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford ; New York : Pergamon Press, c1987-
مواضيع طبية MeSH: Angiogenesis Inhibitors/*pharmacology , Group II Phospholipases A2/*pharmacology , Human Umbilical Vein Endothelial Cells/*drug effects , Reptilian Proteins/*pharmacology, Animals ; Aorta/drug effects ; Bothrops ; Cell Adhesion/drug effects ; Cell Line ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Crotalid Venoms ; Human Umbilical Vein Endothelial Cells/metabolism ; Human Umbilical Vein Endothelial Cells/physiology ; Humans ; Mice ; Vascular Endothelial Growth Factor A/metabolism
مستخلص: Antiangiogenic strategies are promising tools for cancer treatment and several other disorders. In this sense, phospholipases A 2 (PLA 2 s) from snake venom have been described to possess antiangiogenic properties. In this study, we evaluated both in vitro and ex vivo antiangiogenic effects induced by BnSP-7, a Lys49 PLA 2 isolated from Bothrops pauloensis snake venom. BnSP-7 was able to inhibit endothelial cell (HUVEC) proliferation, which was indeed confirmed by a modulation of cell cycle progression. Interestingly, BnSP-7 also inhibited the adhesion and migration of HUVECs and blocked in vitro angiogenesis in a VEGF-dependent manner, an important proangiogenic factor. Finally, BnSP-7 was capable of inhibiting sprouting angiogenic process through an ex vivo aortic ring assay. Taken together, these results indicate that BnSP-7 has potent in vitro and ex vivo antiangiogenic effect.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Antiangiogenic; BnSP-7; Bothrops pauloensis; Cancer; PLA(2)
المشرفين على المادة: 0 (Angiogenesis Inhibitors)
0 (Crotalid Venoms)
0 (Reptilian Proteins)
0 (VEGFA protein, human)
0 (Vascular Endothelial Growth Factor A)
EC 3.1.1.4 (Group II Phospholipases A2)
EC 3.1.1.4 (Lys49-phospholipase A2, Bothrops neuwiedi pauloensis)
تواريخ الأحداث: Date Created: 20210124 Date Completed: 20211022 Latest Revision: 20211022
رمز التحديث: 20231215
DOI: 10.1016/j.tiv.2021.105099
PMID: 33486049
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3177
DOI:10.1016/j.tiv.2021.105099