دورية أكاديمية
أعرض في EDS

Improving the diagnosis of pulmonary tuberculosis using line probe assay and determining the factors associated with the disease in children in Jos, Nigeria.

التفاصيل البيبلوغرافية
العنوان: Improving the diagnosis of pulmonary tuberculosis using line probe assay and determining the factors associated with the disease in children in Jos, Nigeria.
المؤلفون: Ebonyi AO; MBBS, MSc, Department of Paediatrics, University of Jos and Department of Paediatrics, Jos University Teaching Hospital, PMB 2076, Jos, Nigeria., Oguche S; BMBCh, Department of Paediatrics, University of Jos and Department of Paediatrics, Jos University Teaching Hospital, PMB 2076, Jos, Nigeria., Abok II; MBBS, Department of Paediatrics, University of Jos and Department of Paediatrics, Jos University Teaching Hospital, PMB 2076, Jos, Nigeria., Isa YO; BMLS, MSc, MPH, APIN Laboratory, Jos University Teaching Hospital, PMB 2076, Jos, Nigeria., Ani CC; MBBS, Department of Radiology, University of Jos and Department of Radiology, Jos University Teaching Hospital, PMB 2076, Jos, Nigeria., Akhiwu HO; MBBS, Department of Paediatrics, Jos University Teaching Hospital, PMB 2076, Jos, Nigeria., Ihekaike MM; MBBS, Faith Alive Foundation Hospital, P. O. Box 745, Jos, Nigeria., Yiltok ES; MBBS, MEd, Department of Paediatrics, University of Jos and Department of Paediatrics, Jos University Teaching Hospital, PMB 2076, Jos, Nigeria., Ochoga MO; BMBCH, Department of Paediatrics, Benue State University, PMB 102119, Makurdi, Nigeria., Sagay AS; BSc, MBChB, Department of Obstetrics and Gynaecology, University of Jos and Department of Obstetrics and Gynaecology, Jos University Teaching Hospital, PMB 2076, Jos, Nigeria.
المصدر: Germs [Germs] 2020 Dec 28; Vol. 10 (4), pp. 328-337. Date of Electronic Publication: 2020 Dec 28 (Print Publication: 2020).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: European Academy of HIV/AIDS and Infectious Diseases Country of Publication: Romania NLM ID: 101596099 Publication Model: eCollection Cited Medium: Print ISSN: 2248-2997 (Print) Linking ISSN: 22482997 NLM ISO Abbreviation: Germs Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Bucureşti : European Academy of HIV/AIDS and Infectious Diseases
مستخلص: Introduction: Diagnosing tuberculosis (TB), including pulmonary tuberculosis (PTB), in children remains a challenge, partly due to its paucibacillary nature in young children. Data on the use of line probe assay (LPA), on gastric and sputum samples, for diagnosing PTB in children are scarce. We determined the proportion of samples positive for Mycobacterium tuberculosis (MTB) by smear microscopy (SM) and LPA in presumptive PTB cases as well as the factors associated with PTB confirmed by LPA, in children in Jos, Nigeria.
Methods: An observational study in children aged 6 months-16 years. Gastric and sputum samples were examined by SM and by LPA for MTB using GenoType MTBDR plus Ver 2.0 (Hain Lifescience). Multivariate logistic regression was performed to determine the factors associated with PTB.
Results: Out of 103 children with presumptive PTB, 47 had confirmed PTB, 26 unconfirmed PTB and 30 unlikely PTB by LPA. In 67 gastric samples, MTB was identified by SM in 2 (3.0%) compared to 28 (41.8%) by LPA while in 31 sputum samples, MTB was identified by SM in 5 (16.1%) compared to 18 (58.1%) by LPA. The factors associated with pulmonary tuberculosis were an abnormal chest X-ray (adjusted odds ratio (AOR))=12.39 [3.75-40.90], p<0.001), sleeping in the same room with more than three persons (AOR=3.30 [1.23-8.85], p=0.018) and sleeping in a room with none or one window (AOR=2.86 [1.03-7.95], p=0.044).
Conclusions: Line probe assay improves the diagnosis of pulmonary TB in children, especially with gastric samples, while an abnormal chest X-ray is a useful adjunct in PTB diagnosis. Avoiding overcrowding and having windows in sleeping rooms are a necessary part of TB prevention.
Competing Interests: Conflicts of interest: All authors - none to declare.
(GERMS.)
References: Lancet. 2005 Jan 8-14;365(9454):130-4. (PMID: 15639294)
PLoS One. 2009;4(4):e5140. (PMID: 19357770)
Tuberculosis (Edinb). 2003;83(1-3):208-12. (PMID: 12758213)
PLoS One. 2017 Aug 22;12(8):e0182988. (PMID: 28829779)
Clin Infect Dis. 2015 Oct 15;61Suppl 3:S179-87. (PMID: 26409281)
Future Microbiol. 2011 Sep;6(9):1067-82. (PMID: 21958145)
BMC Infect Dis. 2013 Jul 01;13:300. (PMID: 23815441)
PLoS One. 2017 Apr 18;12(4):e0176116. (PMID: 28419146)
Lancet Glob Health. 2017 Sep;5(9):e898-e906. (PMID: 28807188)
BMC Infect Dis. 2016 Jun 13;16:282. (PMID: 27296716)
Arch Dis Child. 1995 Apr;72(4):369-74. (PMID: 7763076)
Int J Tuberc Lung Dis. 2009 Oct;13(10):1231-7. (PMID: 19793427)
J Pediatric Infect Dis Soc. 2013 Jun;2(2):171-4. (PMID: 26619464)
Lancet Infect Dis. 2011 Nov;11(11):819-24. (PMID: 21764384)
J Epidemiol Glob Health. 2017 Jun;7(2):141-145. (PMID: 28188122)
Int J Epidemiol. 2005 Aug;34(4):914-23. (PMID: 15914505)
PLoS Med. 2007 Feb;4(2):e68. (PMID: 17326709)
فهرسة مساهمة: Keywords: Line probe assay; chest X-ray; children; gastric sample; pulmonary tuberculosis; smear microscopy; sputum sample
تواريخ الأحداث: Date Created: 20210125 Latest Revision: 20220420
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7811858
DOI: 10.18683/germs.2020.1225
PMID: 33489948
قاعدة البيانات: MEDLINE
الوصف
تدمد:2248-2997
DOI:10.18683/germs.2020.1225