دورية أكاديمية
Phosphorylation of Specificity Protein 3 Is Critical for Activation of β4-Galactosyltransferase 3 Gene Promoter in SH-SY5Y Human Neuroblastoma Cell Line.
| العنوان: | Phosphorylation of Specificity Protein 3 Is Critical for Activation of β4-Galactosyltransferase 3 Gene Promoter in SH-SY5Y Human Neuroblastoma Cell Line. |
|---|---|
| المؤلفون: | Tange R; Laboratory of Glycobiology, Department of Bioengineering, Nagaoka University of Technology., Tachibana R; Laboratory of Glycobiology, Department of Bioengineering, Nagaoka University of Technology., Sato T; Laboratory of Glycobiology, Department of Bioengineering, Nagaoka University of Technology. |
| المصدر: | Biological & pharmaceutical bulletin [Biol Pharm Bull] 2021 Apr 01; Vol. 44 (4), pp. 557-563. Date of Electronic Publication: 2021 Jan 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pharmaceutical Society of Japan Country of Publication: Japan NLM ID: 9311984 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1347-5215 (Electronic) Linking ISSN: 09186158 NLM ISO Abbreviation: Biol Pharm Bull Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Tokyo : Pharmaceutical Society of Japan, c1993- |
| مواضيع طبية MeSH: | Signal Transduction*, Galactosyltransferases/*metabolism , Neuroblastoma/*metabolism , Sp3 Transcription Factor/*metabolism, Butadienes/pharmacology ; Cell Line, Tumor ; Humans ; Mitogen-Activated Protein Kinase Kinases/metabolism ; Nitriles/pharmacology ; Phosphorylation ; Promoter Regions, Genetic ; Sp3 Transcription Factor/genetics ; Transcriptional Activation |
| مستخلص: | Elevated expression of β4-galactosyltransferase (β4GalT) 3 is correlated with poor clinical outcome of neuroblastoma patients. Our recent study has revealed that the transcription of the β4GalT3 gene is activated by Specificity protein (Sp) 3 in SH-SY5Y human neuroblastoma cell line. Here we report the biological significance of the Sp3 phosphorylation in the transcriptional activation of the β4GalT3 gene. The treatment of SH-SY5Y cells with 10% fetal bovine serum (FBS) increased the mitogen-activated protein kinase (MAPK) signaling and the promoter activity of the β4GalT3 gene. Meanwhile, the treatment with U0126, an inhibitor for MAPK kinase, decreased the MAPK signaling and the promoter activity. These findings indicate that the transcriptional activation of the β4GalT3 gene is mediated by the MAPK signaling. In SH-SY5Y cells cultured in the medium containing 10% FBS, the serine (Ser) residues in Sp3 were phosphorylated. Human Sp3 contains four Ser residues, Ser73, Ser563, Ser566, and Ser646, as the putative phosphorylation sites. Sp3 mutant with the mutation of Ser73 did not decrease the promoter activation of the β4GalT3 gene, indicating that Ser73 is uninvolved in the promoter activation of the β4GalT3 gene by Sp3. In contrast, Sp3 mutants with the mutations of Ser563, Ser566, and Ser646 significantly reduced the promoter activation by Sp3. The results suggest that the phosphorylation of these Ser residues is implicated in the promoter activation by Sp3. This study demonstrates that the phosphorylation of Sp3 plays important roles in the transcriptional activation of the β4GalT3 gene in human neuroblastoma. |
| فهرسة مساهمة: | Keywords: neuroblastoma; phosphorylation; promoter activation; serine; specificity protein 3; β4-galactosyltransferase 3 |
| المشرفين على المادة: | 0 (Butadienes) 0 (Nitriles) 0 (U 0126) 148710-94-5 (Sp3 Transcription Factor) EC 2.4.1.- (Galactosyltransferases) EC 2.4.1.- (beta4-galactosyltransferase III) EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases) |
| تواريخ الأحداث: | Date Created: 20210128 Date Completed: 20211109 Latest Revision: 20211109 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1248/bpb.b20-00906 |
| PMID: | 33504757 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1347-5215 |
|---|---|
| DOI: | 10.1248/bpb.b20-00906 |