دورية أكاديمية
Manool, a diterpene from Salvia officinalis, exerts preventive effects on chromosomal damage and preneoplastic lesions.
| العنوان: | Manool, a diterpene from Salvia officinalis, exerts preventive effects on chromosomal damage and preneoplastic lesions. |
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| المؤلفون: | Nicolella HD; Mutagenesis Laboratory, Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil., Fernandes G; Mutagenesis Laboratory, Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil., Ozelin SD; Mutagenesis Laboratory, Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil., Rinaldi-Neto F; Mutagenesis Laboratory, Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil., Ribeiro AB; Mutagenesis Laboratory, Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil., Furtado RA; Mutagenesis Laboratory, Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil., Senedese JM; Mutagenesis Laboratory, Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil., Esperandim TR; Mutagenesis Laboratory, Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil., Veneziani RCS; Mutagenesis Laboratory, Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil., Tavares DC; Mutagenesis Laboratory, Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil. |
| المصدر: | Mutagenesis [Mutagenesis] 2021 May 31; Vol. 36 (2), pp. 177-185. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8707812 Publication Model: Print Cited Medium: Internet ISSN: 1464-3804 (Electronic) Linking ISSN: 02678357 NLM ISO Abbreviation: Mutagenesis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1995- : Swansea, UK : Oxford University Press Original Publication: Oxford ; Washington, DC : Published for the United Kingdom Environmental Mutagen Society by IRL Press, [1986- |
| مواضيع طبية MeSH: | Anticarcinogenic Agents/*pharmacology , Colonic Neoplasms/*drug therapy , DNA Damage/*drug effects , Diterpenes/*pharmacology , NF-kappa B/*metabolism , Nitric Oxide/*metabolism , Precancerous Conditions/*drug therapy, Animals ; Anticarcinogenic Agents/chemistry ; Cell Line ; Colonic Neoplasms/chemically induced ; Cricetinae ; Disease Models, Animal ; Diterpenes/chemistry ; Dose-Response Relationship, Drug ; Doxorubicin/adverse effects ; Etoposide/adverse effects ; Hydrogen Peroxide/adverse effects ; Male ; Mice ; Micronuclei, Chromosome-Defective/chemically induced ; Micronucleus Tests ; Mutagenicity Tests ; Plant Extracts/pharmacology ; Precancerous Conditions/chemically induced ; Rats ; Rats, Wistar ; Salvia officinalis/chemistry |
| مستخلص: | The present study aimed to evaluate the effect of the manool diterpene on genomic integrity. For this purpose, we evaluated the influence of manool on genotoxicity induced by mutagens with different mechanisms of action, as well as on colon carcinogenesis. The results showed that manool (0.5 and 1.0 µg/ml) significantly reduced the frequency of micronuclei induced by doxorubicin (DXR) and hydrogen peroxide in V79 cells but did not influence genotoxicity induced by etoposide. Mice receiving manool (1.25 mg/kg) exhibited a significant reduction (79.5%) in DXR-induced chromosomal damage. The higher doses of manool (5.0 and 20 mg/kg) did not influence the genotoxicity induced by DXR. The anticarcinogenic effect of manool (0.3125, 1.25 and 5.0 mg/kg) was also observed against preneoplastic lesions chemically induced in rat colon. A gradual increase in manool doses did not cause a proportional reduction of preneoplastic lesions, thus demonstrating the absence of a dose-response relationship. The analysis of serum biochemical indicators revealed the absence of hepatotoxicity and nephrotoxicity of treatments. To explore the chemopreventive mechanisms of manool via anti-inflammatory pathways, we evaluated its effect on nitric oxide (NO) production and on the expression of the NF-kB gene. At the highest concentration tested (4 μg/ml), manool significantly increased NO production when compared to the negative control. On the other hand, in the prophylactic treatment model, manool (0.5 and 1.0 μg/ml) was able to significantly reduce NO levels produced by macrophages stimulated with lipopolysaccharide. Analysis of NF-kB in hepatic and renal tissues of mice treated with manool and DXR revealed that the mutagen was unable to stimulate expression of the gene. In conclusion, manool possesses antigenotoxic and anticarcinogenic effects and its anti-inflammatory potential might be related, at least in part, to its chemopreventive activity. (© The Author(s) 2021. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society.All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| المشرفين على المادة: | 0 (Anticarcinogenic Agents) 0 (Diterpenes) 0 (NF-kappa B) 0 (Plant Extracts) 31C4KY9ESH (Nitric Oxide) 6PLQ3CP4P3 (Etoposide) 80168379AG (Doxorubicin) AT5PJ0PV00 (manool) BBX060AN9V (Hydrogen Peroxide) |
| تواريخ الأحداث: | Date Created: 20210129 Date Completed: 20211217 Latest Revision: 20211217 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1093/mutage/geab001 |
| PMID: | 33512444 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1464-3804 |
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| DOI: | 10.1093/mutage/geab001 |