دورية أكاديمية
Quantitation and Validation of 34 Fentanyl Analogs from Liver Tissue Using a QuEChERS Extraction and LC-MS-MS Analysis.
| العنوان: | Quantitation and Validation of 34 Fentanyl Analogs from Liver Tissue Using a QuEChERS Extraction and LC-MS-MS Analysis. |
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| المؤلفون: | Cox J; Department of Forensic and Investigative Science, 1600 University Avenue, Oglebay Hall, Room 302, Morgantown, WV 26506, USA., Mathison K; Department of Forensic and Investigative Science, 1600 University Avenue, Oglebay Hall, Room 302, Morgantown, WV 26506, USA., Ott C; Department of Forensic and Investigative Science, 1600 University Avenue, Oglebay Hall, Room 302, Morgantown, WV 26506, USA., DelTondo J; Department of Pathology, Allegheny General Hospital, 320 E North Avenue, Pittsburgh, PA 15212, USA., Kraner JC; Office of the Chief Medical Examiner, 619 Virginia Street West, Charleston, WV 25302, USA., DeCaprio AP; Department of Chemistry and Biochemistry, Florida International University (FIU), 11200 SW 8th Street, Miami, FL 33199, USA., Arroyo-Mora LE; Department of Forensic and Investigative Science, 1600 University Avenue, Oglebay Hall, Room 302, Morgantown, WV 26506, USA. |
| المصدر: | Journal of analytical toxicology [J Anal Toxicol] 2022 Mar 21; Vol. 46 (3), pp. 232-245. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 7705085 Publication Model: Print Cited Medium: Internet ISSN: 1945-2403 (Electronic) Linking ISSN: 01464760 NLM ISO Abbreviation: J Anal Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2012- : Oxford : Oxford University Press Original Publication: 1977-<2011>: Niles, Ill., Preston Publications. |
| مواضيع طبية MeSH: | Fentanyl* , Substance Abuse Detection*/methods, Chromatography, Liquid ; Forensic Toxicology/methods ; Liver/chemistry ; Tandem Mass Spectrometry |
| مستخلص: | Since 2013, drug overdose deaths involving synthetic opioids (including fentanyl and fentanyl analogs) have increased from 3,105 to 31,335 in 2018. Postmortem toxicological analysis in fentanyl-related overdose deaths is complicated by the high potency of the drug, often resulting in low analyte concentrations and associations with toxicity, multidrug use, novelty of emerging fentanyl analogs and postmortem redistribution. Objectives for this study include the development of a quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction and subsequent liquid chromatography-mass spectrometry--mass spectrometry analysis, validation of the method following the American Academy of Forensic Sciences Standards Board (ASB) standard 036 requirements and application to authentic liver specimens for 34 analytes including fentanyl, metabolites and fentanyl analogs. The bias for all 34 fentanyl analogs did not exceed ±10% for any of the low, medium or high concentrations and the %CV did not exceed 20%. No interferences were identified. All 34 analytes were within the criteria for acceptable percent ionization suppression or enhancement with the low concentration ranging from -10.2% to 23.7% and the high concentration ranging from -7.1% to 11.0%. Liver specimens from 22 authentic postmortem cases were extracted and analyzed with all samples being positive for at least one target analyte from the 34 compounds. Of the 22 samples, 17 contained fentanyl and metabolites plus at least one fentanyl analog. The highest concentration for a fentanyl analog was 541.4 μg/kg of para-fluoroisobutyryl fentanyl (FIBF). The concentrations for fentanyl (n = 20) ranged between 3.6 and 164.9 μg/kg with a mean of 54.7 μg/kg. The fentanyl analog that was most encountered was methoxyacetyl fentanyl (n = 11) with a range of 0.2-4.6 μg/kg and a mean of 1.3 μg/kg. The QuEChERS extraction was fully validated using the ASB Standard 036 requirements for fentanyl, metabolites and fentanyl analogs in liver tissue. (© The Author(s) 2021. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| معلومات مُعتمدة: | 2019-R2-CX-0037 National Institute of Justice |
| المشرفين على المادة: | UF599785JZ (Fentanyl) |
| تواريخ الأحداث: | Date Created: 20210130 Date Completed: 20220323 Latest Revision: 20220323 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/jat/bkab009 |
| PMID: | 33515247 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1945-2403 |
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| DOI: | 10.1093/jat/bkab009 |