دورية أكاديمية
Thiamethoxam Exposure Induces Endoplasmic Reticulum Stress and Affects Ovarian Function and Oocyte Development in Mice.
| العنوان: | Thiamethoxam Exposure Induces Endoplasmic Reticulum Stress and Affects Ovarian Function and Oocyte Development in Mice. |
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| المؤلفون: | Liu Y; Medical College, Guangxi University, Nanning, Guangxi 530004, People's Republic of China., He QK; Medical College, Guangxi University, Nanning, Guangxi 530004, People's Republic of China., Xu ZR; Center for Translational Medicine Research, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi 530011, People's Republic of China., Xu CL; Reproductive Medical Center of Nanning Second People's Hospital, Nanning, Guangxi 530031, People's Republic of China., Zhao SC; Medical College, Guangxi University, Nanning, Guangxi 530004, People's Republic of China., Luo YS; Medical College, Guangxi University, Nanning, Guangxi 530004, People's Republic of China., Sun X; Medical College, Guangxi University, Nanning, Guangxi 530004, People's Republic of China., Qi ZQ; Medical College, Guangxi University, Nanning, Guangxi 530004, People's Republic of China., Wang HL; Department of Basic Medicine, School of Medicine, Xiamen University, Xiamen, Fujian 361102, People's Republic of China. |
| المصدر: | Journal of agricultural and food chemistry [J Agric Food Chem] 2021 Feb 17; Vol. 69 (6), pp. 1942-1952. Date of Electronic Publication: 2021 Feb 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Endoplasmic Reticulum Stress* , Insecticides*/toxicity, Animals ; Female ; Mice ; Neonicotinoids/toxicity ; Nitro Compounds/toxicity ; Oocytes ; Thiamethoxam |
| مستخلص: | Neonicotinoids are the most widely used insecticides in modern agriculture, and their residues have been found in the environment and food. Previous studies reported that neonicotinoids exert toxic effects in various tissues, but whether they interfered with the female reproductive process remains unknown. In our present research, thiamethoxam was selected as a representative neonicotinoid to establish a mouse toxicity model with gavage. We found that thiamethoxam decreased the ovarian coefficient and disrupted the expression of female hormone receptors, subsequently affecting follicle development. Ovarian granulosa cells from the thiamethoxam exposure group underwent a high level of apoptosis. Using transcriptome analysis, we showed that thiamethoxam exposure altered the expression of multiple oocyte genes related to inflammation, apoptosis, and endoplasmic reticulum stress. Thiamethoxam also adversely affected oocyte and embryo development. Western blotting and fluorescence staining results confirmed that thiamethoxam affected the integrity of DNA, triggered apoptosis, promoted oxidative stress and endoplasmic reticulum stress, and impaired mitochondrial function. Collectively, our results indicated that thiamethoxam exposure disrupts ovarian homeostasis and decreases oocyte quality via endoplasmic reticulum stress and apoptosis induction. |
| فهرسة مساهمة: | Keywords: ER stress; apoptosis; mice; oocytes; thiamethoxam exposure |
| المشرفين على المادة: | 0 (Insecticides) 0 (Neonicotinoids) 0 (Nitro Compounds) 747IC8B487 (Thiamethoxam) |
| تواريخ الأحداث: | Date Created: 20210203 Date Completed: 20210514 Latest Revision: 20210514 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1021/acs.jafc.0c06340 |
| PMID: | 33533595 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5118 |
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| DOI: | 10.1021/acs.jafc.0c06340 |