دورية أكاديمية
Equine maternal aging affects oocyte lipid content, metabolic function and developmental potential.
| العنوان: | Equine maternal aging affects oocyte lipid content, metabolic function and developmental potential. |
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| المؤلفون: | Catandi GD; Equine Reproduction Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA., Obeidat YM; Electronic Engineering Department, Hijjawi Faculty for Engineering Technology, Yarmouk University, Irbid, Jordan., Broeckling CD; Proteomics and Metabolomics Facility, Colorado State University, Fort Collins, Colorado, USA., Chen TW; Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado, USA., Chicco AJ; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA., Carnevale EM; Equine Reproduction Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA. |
| المصدر: | Reproduction (Cambridge, England) [Reproduction] 2021 Apr; Vol. 161 (4), pp. 399-409. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published for the Society for Reproduction and Fertility by BioScientifica Country of Publication: England NLM ID: 100966036 Publication Model: Print Cited Medium: Internet ISSN: 1741-7899 (Electronic) Linking ISSN: 14701626 NLM ISO Abbreviation: Reproduction Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2004->: Bristol, UK : Published for the Society for Reproduction and Fertility by BioScientifica Original Publication: Cambridge, UK : Journals of Reproduction and Fertility, Ltd. c2001- |
| مواضيع طبية MeSH: | Gene Expression Regulation, Developmental* , Maternal Age* , Oogenesis*, Cumulus Cells/*pathology , In Vitro Oocyte Maturation Techniques/*veterinary , Lipids/*analysis , Mitochondria/*pathology , Oocytes/*pathology, Animals ; Cumulus Cells/metabolism ; DNA, Mitochondrial/analysis ; DNA, Mitochondrial/genetics ; Female ; Horses ; Mitochondria/metabolism ; Oocytes/metabolism ; Oxygen Consumption |
| مستخلص: | Advanced maternal age is associated with a decline in fertility and oocyte quality. We used novel metabolic microsensors to assess effects of mare age on single oocyte and embryo metabolic function, which has not yet been similarly investigated in mammalian species. We hypothesized that equine maternal aging affects the metabolic function of oocytes and in vitro-produced early embryos, oocyte mitochondrial DNA (mtDNA) copy number, and relative abundance of metabolites involved in energy metabolism in oocytes and cumulus cells. Samples were collected from preovulatory follicles from young (≤14 years) and old (≥20 years) mares. Relative abundance of metabolites in metaphase II oocytes (MII) and their respective cumulus cells, detected by liquid and gas chromatography coupled to mass spectrometry, revealed that free fatty acids were less abundant in oocytes and more abundant in cumulus cells from old vs young mares. Quantification of aerobic and anaerobic metabolism, respectively measured as oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in a microchamber containing oxygen and pH microsensors, demonstrated reduced metabolic function and capacity in oocytes and day-2 embryos originating from oocytes of old when compared to young mares. In mature oocytes, mtDNA was quantified by real-time PCR and was not different between the age groups and not indicative of mitochondrial function. Significantly more sperm-injected oocytes from young than old mares resulted in blastocysts. Our results demonstrate a decline in oocyte and embryo metabolic activity that potentially contributes to the impaired developmental competence and fertility in aged females. |
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| معلومات مُعتمدة: | R21 HD097601 United States HD NICHD NIH HHS |
| المشرفين على المادة: | 0 (DNA, Mitochondrial) 0 (Lipids) |
| تواريخ الأحداث: | Date Created: 20210204 Date Completed: 20220103 Latest Revision: 20240331 |
| رمز التحديث: | 20240331 |
| مُعرف محوري في PubMed: | PMC7969451 |
| DOI: | 10.1530/REP-20-0494 |
| PMID: | 33539317 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1741-7899 |
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| DOI: | 10.1530/REP-20-0494 |