دورية أكاديمية
Development of a Luminex microbead-based serotyping assay for Glaesserella parasuis.
| العنوان: | Development of a Luminex microbead-based serotyping assay for Glaesserella parasuis. |
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| المؤلفون: | Yee S; The University of Queensland, Queensland Alliance for Agriculture and Food Innovation, St Lucia, Qld 4067, Australia., Meers J; The University of Queensland, School of Veterinary Science, Gatton, QLD 4343, Australia., Parke CR; The University of Queensland, School of Veterinary Science, Gatton, QLD 4343, Australia., Barnes TS; The University of Queensland, Queensland Alliance for Agriculture and Food Innovation, St Lucia, Qld 4067, Australia; The University of Queensland, School of Veterinary Science, Gatton, QLD 4343, Australia., Blackall PJ; The University of Queensland, Queensland Alliance for Agriculture and Food Innovation, St Lucia, Qld 4067, Australia., Turni C; The University of Queensland, Queensland Alliance for Agriculture and Food Innovation, St Lucia, Qld 4067, Australia. Electronic address: c.turni1@uq.edu.au. |
| المصدر: | Journal of microbiological methods [J Microbiol Methods] 2021 Mar; Vol. 182, pp. 106159. Date of Electronic Publication: 2021 Feb 04. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, The Netherlands : Elsevier Biomedical, c1983- |
| مواضيع طبية MeSH: | Haemophilus Infections*/diagnosis , Haemophilus Infections*/microbiology , Serogroup* , Swine Diseases*/diagnosis , Swine Diseases*/microbiology, Haemophilus parasuis/*isolation & purification , Serotyping/*methods, Animals ; Australia ; Swine |
| مستخلص: | Glaesserella parasuis consists of 15 serovars with some of them highly virulent and some of them avirulent. As killed vaccines do not provide crossprotection across serovars, serotyping is of importance. Serotyping, previously done by gel diffusion, is now done by multiplex PCR followed by electrophoresis. Accurately differentiating 15 serovars by electrophoresis is problematic. To overcome this problem, a Luminex microbead-based multiplex assay was used to differentiate the serovars. The assay consisted of a multiplex PCR assay followed by hybridisation to microbeads which were then analysed on a Luminex machine. The newly developed assay was compared to the multiplex serotyping PCR and the gel diffusion/indirect haemagglutination assay (GD/IHA). The microbead-based assay worked very well for the 15 reference strains but when used on the 74 Australian field strains displayed some problems. The main problems were with the eight out of nine serovar 4 field isolates and the five serovar 7 and three serovar 14 field isolates. While the microbead-based assay could differentiate between the serovar 5 and 12 reference strains, which the serovar multiplex PCR could not, all four field isolates identified by GD/IHA as serovar 12 were identified as serovar 5 by the microbead-based assay. Serovar 4 has been noted to have a high diversity especially among strains from different countries. Our work clearly shows that the diversity of strains at both the national and the international level has to be taken into account when developing diagnostic assays. (Copyright © 2021 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Glaesserella parasuis; Luminex; Multiplex PCR; Serovars |
| تواريخ الأحداث: | Date Created: 20210206 Date Completed: 20211001 Latest Revision: 20211001 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.mimet.2021.106159 |
| PMID: | 33548394 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1872-8359 |
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| DOI: | 10.1016/j.mimet.2021.106159 |