دورية أكاديمية
High-speed centrifugation of extender of freeze-thaw boar semen.
| العنوان: | High-speed centrifugation of extender of freeze-thaw boar semen. |
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| المؤلفون: | de Brito CRC; ReproPel Research Group, Veterinary Faculty, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil., Varela Junior AS; RAC, Reprodução Animal Comparada, Instituto de Ciências Biológicas, Universidade Federal de Rio Grande (FURG), Rio Grande, Brazil., Gheller SMM; ReproPel Research Group, Veterinary Faculty, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil., Acosta IB; ReproPel Research Group, Veterinary Faculty, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil., Anciuti AN; ReproPel Research Group, Veterinary Faculty, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil., Gatti NLS; ReproPel Research Group, Veterinary Faculty, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil., Silva EA; ReproPel Research Group, Veterinary Faculty, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil., Knabah NW; ReproPel Research Group, Veterinary Faculty, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil., Corcini CD; ReproPel Research Group, Veterinary Faculty, Universidade Federal de Pelotas (UFPel), Pelotas, Brazil. |
| المصدر: | Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2021 May; Vol. 56 (5), pp. 821-825. Date of Electronic Publication: 2021 Feb 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Paul Parey Scientific Publishers Country of Publication: Germany NLM ID: 9015668 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0531 (Electronic) Linking ISSN: 09366768 NLM ISO Abbreviation: Reprod Domest Anim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin ; Hamburg : Paul Parey Scientific Publishers, c1990- |
| مواضيع طبية MeSH: | Cryopreservation/*veterinary , Semen Preservation/*veterinary , Swine/*physiology, Animals ; Centrifugation/methods ; Centrifugation/veterinary ; Cryopreservation/methods ; Cryoprotective Agents ; Egg Yolk/chemistry ; Freezing ; Male ; Semen Preservation/methods ; Sonication/methods ; Sonication/veterinary ; Sperm Motility ; Spermatozoa/cytology |
| مستخلص: | In semen cryopreservation, egg yolk is still widely used as a non-penetrating cryoprotectant. Much has been developed in the search for alternatives for this biological product. This work aimed to evaluate the processed egg yolk through ultracentrifugation and/or sonication in the cryopreservation of swine semen. Twenty-seven semen doses were purchased from a commercial boar stud and processed for cryopreservation using egg yolk lactose 11% (control) extender, processed using two different methods: high-speed centrifugation and sonication. Then, they were submitted to freeze-thawing protocol and were assessed for kinematic and cell structural parameters. Samples in which extenders underwent centrifugation had better results in velocity parameters, meanwhile those that only sonication was performed had poorest results in this parameter. The preservation of the membrane and mitochondria structure had better results when the diluent was only centrifuged in comparison with the other treatments. Therefore, centrifugation of extender containing egg yolk is important for better cryopreservation of swine semen. (© 2021 Wiley-VCH GmbH.) |
| References: | Abismail, B., Canselier, J. P., Wilhelm, A. M., Delmas, H., & Gourdon, C. (1999). Emulsification by ultrasound: Drop size distribution and stability. Ultrasonics Sonochemistry, 6(1-2), 75-83. https://doi.org/10.1016/S1350-4177(98)00027-3. Amirat, L., Tainturier, D., Jeanneau, L., Thorin, C., Gérard, O., Courtens, J. L., & Anton, M. (2004). Bull semen in vitro fertility after cryopreservation using egg yolk LDL: A comparison with Optidyl®, a commercial egg yolk extender. Theriogenology, 61(5), 895-907. https://doi.org/10.1016/s0093-691x(03)00259-0. Chanapiwat, P., Kaeoket, K., & Tummaruk, P. (2012). Cryopreservation of boar semen by egg yolk-based extenders containing lactose or fructose is better than sorbitol. Journal of Veterinary Medical Science, 74(3), 351-354. https://doi.org/10.1292/jvms.11-0273. Evenson, D., Jost, L., Marshall, D., Zinaman, M., Clegg, E., Purvis, K., & Claussen, O. (1999). Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Human Reproduction, 14(4), 1039-1049. https://doi.org/10.1093/humrep/14.4.1039. Garner, D. L., Thomas, C. A., Joerg, H. W., DeJarnette, J. M., & Marshall, C. E. (1997). Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biology of Reproduction, 57(6), 1401-1406. Harrison, R., & Vickers, S. E. (1990). Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. Journal of Reproduction and Fertility, 88(1), 343-352. https://doi.org/10.1530/jrf.0.0880343. He, L., Bailey, J. L., & Buhr, M. M. (2001). Incorporating lipids into boar sperm decreases chilling sensitivity but not capacitation potential. Biology of Reproduction, 64, 69-79. Hu, J.-H., Li, Q.-W., Li, G., Chen, X.-Y., Hai-Yang, H.-Y., Zhang, S.-S., & Wang, L.-Q. (2006). The cryoprotective effect on frozen-thawed boar semen of egg yolk low density lipoproteins. Asian-australasian Journal of Animal Sciences, 19(4), 486-494. https://doi.org/10.5713/ajas.2006.486. Kawamoto, A., Ohashi, K., Kishikawa, H., Zhu, L. Q., Azuma, C., & Murata, Y. (1999). Two-color fluorescence staining of lectin and anti-CD46 antibody to assess acrosomal status. Fertility and Sterility, 71(3), 497-501. https://doi.org/10.1016/S0015-0282(98)00507-X. Kentish, S., Wooster, T., Ashokkumar, M., Balachandran, S., Mawson, R., & Simons, L. (2008). The use of ultrasonics for nanoemulsion preparation. Innovative Food Science & Emerging Technologies, 9(2), 170-175. https://doi.org/10.1016/j.ifset.2007.07.005. Knox, R. V. (2015). The fertility of frozen boar sperm when used for artificial insemination. Reproduction in Domestic Animals, 50, 90-97. https://doi.org/10.1111/rda.12552. Knox, R., Levis, D., Safranski, T., & Singleton, W. (2008). An update on North American boar stud practices. Theriogenology, 70(8), 1202-1208. https://doi.org/10.1016/j.theriogenology.2008.06.036. Li, J., Roca, J., Perez-Patino, C., Barranco, I., Martinez, E. A., Rodriguez-Martinez, H., & Parrilla, I. (2018). Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma? Animal Reproduction Science, 195, 30-37. https://doi.org/10.1016/j.anireprosci.2018.05.002. Pillet, E., Labbe, C., Batellier, F., Duchamp, G., Beaumal, V., Anton, M., Desherces, S., Schmitt, E., & Magistrini, M. (2012). Liposomes as an alternative to egg yolk in stallion freezing extender. Theriogenology, 77(2), 268-279. https://doi.org/10.1016/j.theriogenology.2011.08.001. Puisieux, F., Fattal, E., Lahiani, M., Auger, J., Jouannet, P., Couvreur, P., & Delattre, J. (1994). Liposomes, an interesting tool to deliver a bioenergetic substrate (ATP). in vitro and in vivo studies. Journal of Drug Targeting, 2(5), 443-448. https://doi.org/10.3109/10611869408996820. Tosic, J., & Walton, A. (1947). Effect of egg yolk and its constituents on the respiration and fertilizing capacity of spermatozoa. The Journal of Agricultural Science, 37(1), 69-76. https://doi.org/10.1017/S0021859600013095. Varela Junior, A. S., Corcini, C. D., Ulguim, R. R., Alvarenga, M., Bianchi, I., Corrêa, M. N., Lucia, T., & Deschamps, J. C. (2009). Effect of low density lipoprotein on the quality of cryopreserved dog semen. Animal Reproduction Science, 115(1-4), 323-327. https://doi.org/10.1016/j.anireprosci.2008.11.002. Watson, P. F. (1995). Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reproduction, Fertility and Development, 7(4), 871-891. https://doi.org/10.1071/RD9950871. Watson, P., & Martin, I. (1975). The influence of some fractions of egg yolk on the survival of ram spermatozoa at 5oC. Australian Journal of Biological Sciences, 28(2), 145-152. https://doi.org/10.1071/BI9750145. Yeste, M., Estrada, E., Pinart, E., Bonet, S., Miró, J. & Rodríguez-Gil, J.E. (2014). The improving effect of reduced glutathione on boar sperm cryotolerance is related with the intrinsic ejaculate freezability. Cryobiology, 68(2), 251-261. https://doi.org/10.1016/j.cryobiol.2014.02.004. |
| معلومات مُعتمدة: | 1725510000940-1 Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul; Antonio Sergio - 310327/2018-0 Conselho Nacional de Desenvolvimento Científico e Tecnológico; Carine Corcini 310203/2018-0 Conselho Nacional de Desenvolvimento Científico e Tecnológico; Universal - 425546/2018-7 Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| فهرسة مساهمة: | Keywords: boar; cryopreservation; high-speed centrifugation; kinematic parameters; semen; sonication |
| المشرفين على المادة: | 0 (Cryoprotective Agents) |
| تواريخ الأحداث: | Date Created: 20210211 Date Completed: 20210924 Latest Revision: 20210924 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/rda.13915 |
| PMID: | 33569811 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1439-0531 |
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| DOI: | 10.1111/rda.13915 |