دورية أكاديمية
The effects of high fat diet, bone healing, and BMP-2 treatment on endothelial cell growth and function.
العنوان: | The effects of high fat diet, bone healing, and BMP-2 treatment on endothelial cell growth and function. |
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المؤلفون: | Bhatti FUR; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA; Richard L. Roudebush VA Medical Center, IN, USA., Dadwal UC; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA; Richard L. Roudebush VA Medical Center, IN, USA., Valuch CR; Department of Biology, Indiana University Purdue University Indianapolis, IN, USA., Tewari NP; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA., Awosanya OD; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA., de Andrade Staut C; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA., Sun S; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA., Mendenhall SK; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA., Perugini AJ 3rd; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA., Nagaraj RU; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA., Battina HL; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA., Nazzal MK; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA., Blosser RJ; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA; Richard L. Roudebush VA Medical Center, IN, USA., Maupin KA; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA., Childress PJ; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA; Richard L. Roudebush VA Medical Center, IN, USA., Li J; Department of Biology, Indiana University Purdue University Indianapolis, IN, USA., Kacena MA; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA; Richard L. Roudebush VA Medical Center, IN, USA. Electronic address: mkacena@iupui.edu. |
المصدر: | Bone [Bone] 2021 May; Vol. 146, pp. 115883. Date of Electronic Publication: 2021 Feb 11. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 8504048 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2763 (Electronic) Linking ISSN: 18732763 NLM ISO Abbreviation: Bone Subsets: MEDLINE |
أسماء مطبوعة: | Publication: New York : Elsevier Science Original Publication: Elmsford, NY : Pergamon Press, c1985- |
مواضيع طبية MeSH: | Diabetes Mellitus, Experimental* , Femoral Fractures*, Animals ; Cell Proliferation ; Diet, High-Fat/adverse effects ; Endothelial Cells ; Mice |
مستخلص: | Angiogenesis is a vital process during the regeneration of bone tissue. The aim of this study was to investigate angiogenesis at the fracture site as well as at distal locations from obesity-induced type 2 diabetic mice that were treated with bone morphogenetic protein-2 (BMP-2, local administration at the time of surgery) to heal a femoral critical sized defect (CSD) or saline as a control. Mice were fed a high fat diet (HFD) to induce a type 2 diabetic-like phenotype while low fat diet (LFD) animals served as controls. Endothelial cells (ECs) were isolated from the lungs (LECs) and bone marrow (BMECs) 3 weeks post-surgery, and the fractured femurs were also examined. Our studies demonstrate that local administration of BMP-2 at the fracture site in a CSD model results in complete bone healing within 3 weeks for all HFD mice and 66.7% of LFD mice, whereas those treated with saline remain unhealed. At the fracture site, vessel parameters and adipocyte numbers were significantly increased in BMP-2 treated femurs, irrespective of diet. At distal sites, LEC and BMEC proliferation was not altered by diet or BMP-2 treatment. HFD increased the tube formation ability of both LECs and BMECs. Interestingly, BMP-2 treatment at the time of surgery reduced tube formation in LECs and humeri BMECs. However, migration of BMECs from HFD mice treated with BMP-2 was increased compared to BMECs from HFD mice treated with saline. BMP-2 treatment significantly increased the expression of CD31, FLT-1, and ANGPT2 in LECs and BMECs in LFD mice, but reduced the expression of these same genes in HFD mice. To date, this is the first study that depicts the systemic influence of fracture surgery and local BMP-2 treatment on the proliferation and angiogenic potential of ECs derived from the bone marrow and lungs. (Published by Elsevier Inc.) |
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معلومات مُعتمدة: | R01 AG060621 United States AG NIA NIH HHS; T32 HL007910 United States HL NHLBI NIH HHS; P30 DK097512 United States DK NIDDK NIH HHS; U54 DK106846 United States DK NIDDK NIH HHS; I01 BX003751 United States BX BLRD VA; T32 DK007519 United States DK NIDDK NIH HHS |
فهرسة مساهمة: | Keywords: Angiogenesis; Bone; Bone healing; Bone morphogenetic protein; Bone regeneration; Endothelial cells; Fracture healing; High fat diet; Type 2 diabetes |
تواريخ الأحداث: | Date Created: 20210213 Date Completed: 20210708 Latest Revision: 20240407 |
رمز التحديث: | 20240407 |
مُعرف محوري في PubMed: | PMC8009863 |
DOI: | 10.1016/j.bone.2021.115883 |
PMID: | 33581374 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1873-2763 |
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DOI: | 10.1016/j.bone.2021.115883 |