Targeted Gene Editing in Porcine Spermatogonia.

التفاصيل البيبلوغرافية
العنوان:	Targeted Gene Editing in Porcine Spermatogonia.
المؤلفون:	Webster D; Recombinetics, Inc., St. Paul, MN, United States., Bondareva A; Department of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, Canada., Solin S; Recombinetics, Inc., St. Paul, MN, United States., Goldsmith T; Recombinetics, Inc., St. Paul, MN, United States., Su L; Department of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, Canada., Lara NLEM; Department of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, Canada., Carlson DF; Recombinetics, Inc., St. Paul, MN, United States., Dobrinski I; Department of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, Canada.
المصدر:	Frontiers in genetics [Front Genet] 2021 Jan 28; Vol. 11, pp. 627673. Date of Electronic Publication: 2021 Jan 28 (Print Publication: 2020).
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101560621 Publication Model: eCollection Cited Medium: Print ISSN: 1664-8021 (Print) Linking ISSN: 16648021 NLM ISO Abbreviation: Front Genet Subsets: PubMed not MEDLINE
أسماء مطبوعة:	Original Publication: Lausanne : Frontiers Research Foundation.
مستخلص:	To study the pathophysiology of human diseases, develop innovative treatments, and refine approaches for regenerative medicine require appropriate preclinical models. Pigs share physiologic and anatomic characteristics with humans and are genetically more similar to humans than are mice. Genetically modified pigs are essential where rodent models do not mimic the human disease phenotype. The male germline stem cell or spermatogonial stem cell (SSC) is unique; it is the only cell type in an adult male that divides and contributes genes to future generations, making it an ideal target for genetic modification. Here we report that CRISPR/Cas9 ribonucleoprotein (RNP)-mediated gene editing in porcine spermatogonia that include SSCs is significantly more efficient than previously reported editing with TALENs and allows precise gene editing by homology directed repair (HDR). We also established homology-mediated end joining (HMEJ) as a second approach to targeted gene editing to enable introduction of larger transgenes and/or humanizing parts of the pig genome for disease modeling or regenerative medicine. In summary, the approaches established in the current study result in efficient targeted genome editing in porcine germ cells for precise replication of human disease alleles. Competing Interests: DW, SS, TG, DC, and ID are employees and/or shareholders in Recombinetics, Inc., a company that commercializes gene editing in livestock. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Webster, Bondareva, Solin, Goldsmith, Su, Lara, Carlson and Dobrinski.)
References:	Reproduction. 2006 Oct;132(4):617-24. (PMID: 17008473) J Cell Physiol. 2009 Aug;220(2):460-8. (PMID: 19388011) Sci Rep. 2015 Sep 18;5:14253. (PMID: 26381350) Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25677-25687. (PMID: 31754036) Bioinformatics. 2015 Dec 15;31(24):4014-6. (PMID: 26358729) FASEB J. 2008 Feb;22(2):374-82. (PMID: 17873102) Reproduction. 2001 Mar;121(3):347-54. (PMID: 11226060) J Anim Sci Biotechnol. 2020 Apr 10;11:33. (PMID: 32308978) Commun Biol. 2018 Oct 2;1:158. (PMID: 30302402) Stem Cell Reports. 2015 Jul 14;5(1):75-82. (PMID: 26095606) Cell Res. 2015 Jan;25(1):67-79. (PMID: 25475058) Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11303-7. (PMID: 7972054) Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11298-302. (PMID: 7972053) Diabetes. 2013 May;62(5):1505-11. (PMID: 23274907) Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17382-7. (PMID: 23027955) J Surg Res. 2018 Sep;229:28-40. (PMID: 29937002) Methods Mol Biol. 2010;649:247-56. (PMID: 20680839) Diabetologia. 2017 Aug;60(8):1541-1549. (PMID: 28480495) Adv Genet. 2012;80:37-97. (PMID: 23084873) Stem Cells Dev. 2017 Aug 1;26(15):1121-1131. (PMID: 28474535) Reproduction. 2017 Sep;154(3):181-195. (PMID: 28576919) Biol Reprod. 2002 Jan;66(1):21-8. (PMID: 11751259) Stem Cells. 2006 Apr;24(4):805-14. (PMID: 16282443) Bioinformatics. 2014 May 15;30(10):1473-5. (PMID: 24463181) Biol Reprod. 2013 Jan 31;88(1):27. (PMID: 23221397) Elife. 2020 May 15;9:. (PMID: 32412410) Mol Reprod Dev. 2008 Feb;75(2):250-64. (PMID: 17595009) Cell Rep. 2015 Mar 24;10(11):1828-35. (PMID: 25772367) Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14931-6. (PMID: 12391306) Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16526-31. (PMID: 24014591) J Androl. 2005 Nov-Dec;26(6):698-705. (PMID: 16291964) Reprod Fertil Dev. 2009;21(1):60-6. (PMID: 19152746) Biol Reprod. 2001 Jul;65(1):23-30. (PMID: 11420219) Biol Reprod. 2002 Jul;67(1):55-63. (PMID: 12079999) DNA Repair (Amst). 2018 Oct;70:55-66. (PMID: 30179733) Mol Reprod Dev. 2018 Mar;85(3):250-261. (PMID: 29393557) J Vis Exp. 2019 Oct 3;(152):. (PMID: 31633676) Science. 2008 Sep 26;321(5897):1837-41. (PMID: 18818360) Mol Reprod Dev. 2012 Apr;79(4):255-61. (PMID: 22231935) Biol Reprod. 2002 Sep;67(3):874-9. (PMID: 12193397) Proc Natl Acad Sci U S A. 2020 Sep 29;117(39):24195-24204. (PMID: 32929012) Theriogenology. 2006 Dec;66(9):2091-103. (PMID: 16870245) Anim Reprod. 2015 Jan-Mar;12(1):93-104. (PMID: 27390591)
معلومات مُعتمدة:	R01 HD091068 United States HD NICHD NIH HHS; R01 OD016575 United States OD NIH HHS; R43 GM108150 United States GM NIGMS NIH HHS; R44 GM108150 United States GM NIGMS NIH HHS
فهرسة مساهمة:	Keywords: CRISPR/Cas9; gene targeting; homology directed repair; homology-mediated end joining; pig; spermatogonia
تواريخ الأحداث:	Date Created: 20210215 Latest Revision: 20211230
رمز التحديث:	20240628
مُعرف محوري في PubMed:	PMC7876475
DOI:	10.3389/fgene.2020.627673
PMID:	33584819
قاعدة البيانات:	MEDLINE

Full Text Finder

الوصف
تدمد:	1664-8021
DOI:	10.3389/fgene.2020.627673