دورية أكاديمية
Enrichment of Circular RNA Expression Deregulation at the Transition to Recurrent Spontaneous Seizures in Experimental Temporal Lobe Epilepsy.
| العنوان: | Enrichment of Circular RNA Expression Deregulation at the Transition to Recurrent Spontaneous Seizures in Experimental Temporal Lobe Epilepsy. |
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| المؤلفون: | Gomes-Duarte A; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, University Medical Center Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Bauer S; Epilepsy Center Frankfurt Rhine-Main, Neurocenter, University Hospital Frankfurt and Center for Personalized Translational Epilepsy Research, Goethe-University Frankfurt, Frankfurt, Germany.; Epilepsy Center, Department of Neurology, Philipps University Marburg, Marburg, Germany., Venø MT; Interdisciplinary Nanoscience Centre, Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.; Omiics ApS, Aarhus, Denmark., Norwood BA; Department of Neuroscience, Expesicor Inc., Kalispell, MT, United States.; Diagnostics Development, FYR Diagnostics, Missoula, MT, United States., Henshall DC; Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.; FutureNeuro, The Science Foundation Ireland Research Centre for Chronic and Rare Neurological Diseases, Royal College of Surgeons in Ireland, Dublin, Ireland., Kjems J; Interdisciplinary Nanoscience Centre, Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark., Rosenow F; Epilepsy Center Frankfurt Rhine-Main, Neurocenter, University Hospital Frankfurt and Center for Personalized Translational Epilepsy Research, Goethe-University Frankfurt, Frankfurt, Germany.; Epilepsy Center, Department of Neurology, Philipps University Marburg, Marburg, Germany., Vangoor VR; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, University Medical Center Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Pasterkamp RJ; Affiliated Partner of the European Reference Network EpiCARE, Department of Translational Neuroscience, University Medical Center Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands. |
| المصدر: | Frontiers in genetics [Front Genet] 2021 Jan 28; Vol. 12, pp. 627907. Date of Electronic Publication: 2021 Jan 28 (Print Publication: 2021). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101560621 Publication Model: eCollection Cited Medium: Print ISSN: 1664-8021 (Print) Linking ISSN: 16648021 NLM ISO Abbreviation: Front Genet Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation. |
| مستخلص: | Mesial temporal lobe epilepsy (mTLE) is a common form of epilepsy and is characterized by recurrent spontaneous seizures originating from the temporal lobe. The majority of mTLE patients develop pharmacoresistance to available anti-epileptic drugs (AEDs) while exhibiting severe pathological changes that can include hippocampal atrophy, neuronal death, gliosis and chronic seizures. The molecular mechanisms leading to mTLE remain incompletely understood, but are known to include defects in post-transcriptional gene expression regulation, including in non-coding RNAs (ncRNAs). Circular RNAs (circRNAs) are a class of recently rediscovered ncRNAs with high levels of expression in the brain and proposed roles in diverse neuronal processes. To explore a potential role for circRNAs in epilepsy, RNA-sequencing (RNA-seq) was performed on hippocampal tissue from a rat perforant pathway stimulation (PPS) model of TLE at different post-stimulation time points. This analysis revealed 218 differentially expressed (DE) circRNAs. Remarkably, the majority of these circRNAs were changed at the time of the occurrence of the first spontaneous seizure (DOFS). The expression pattern of two circRNAs, circ_Arhgap4 and circ_Nav3, was further validated and linked to miR-6328 and miR-10b-3p target regulation, respectively. This is the first study to examine the regulation of circRNAs during the development of epilepsy. It reveals an intriguing link between circRNA deregulation and the transition of brain networks into the state of spontaneous seizure activity. Together, our results provide a molecular framework for further understanding the role and mechanism-of-action of circRNAs in TLE. Competing Interests: MV was employed by the company Omiics ApS. BN was employed by the companies Expesicor Inc. and FYR Diagnostics. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Gomes-Duarte, Bauer, Venø, Norwood, Henshall, Kjems, Rosenow, Vangoor and Pasterkamp.) |
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| فهرسة مساهمة: | Keywords: RNA-sequencing; circular RNA; early epilepsy; epileptogenesis; microRNA; network analysis; temporal lobe epilepsy |
| تواريخ الأحداث: | Date Created: 20210215 Latest Revision: 20240806 |
| رمز التحديث: | 20240806 |
| مُعرف محوري في PubMed: | PMC7876452 |
| DOI: | 10.3389/fgene.2021.627907 |
| PMID: | 33584828 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-8021 |
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| DOI: | 10.3389/fgene.2021.627907 |