دورية أكاديمية
أعرض في EDS

Determination of ketone bodies in biological samples via rapid UPLC-MS/MS.

التفاصيل البيبلوغرافية
العنوان: Determination of ketone bodies in biological samples via rapid UPLC-MS/MS.
المؤلفون: Puchalska P; Division of Molecular Medicine, Department of Medicine, University of Minnesota, Minneapolis, MN, USA. Electronic address: ppuchals@umn.edu., Nelson AB; Division of Molecular Medicine, Department of Medicine, University of Minnesota, Minneapolis, MN, USA; Bioinformatics and Computational Biology Program, University of Minnesota, Minneapolis, MN, USA., Stagg DB; Division of Molecular Medicine, Department of Medicine, University of Minnesota, Minneapolis, MN, USA; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, USA., Crawford PA; Division of Molecular Medicine, Department of Medicine, University of Minnesota, Minneapolis, MN, USA; Bioinformatics and Computational Biology Program, University of Minnesota, Minneapolis, MN, USA; Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, USA.
المصدر: Talanta [Talanta] 2021 Apr 01; Vol. 225, pp. 122048. Date of Electronic Publication: 2020 Dec 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
مواضيع طبية MeSH: Ketone Bodies* , Tandem Mass Spectrometry*, 3-Hydroxybutyric Acid ; Animals ; Chromatography, High Pressure Liquid ; Chromatography, Liquid
مستخلص: Efforts to enhance wellness and ameliorate disease via nutritional, chronobiological, and pharmacological interventions have markedly intensified interest in ketone body metabolism. The two ketone body redox partners, acetoacetate (AcAc) and D-β-hydroxybutyrate (D-βOHB) serve distinct metabolic and signaling roles in biological systems. A highly efficient, specific, and reliable approach to simultaneously quantify AcAc and D-βOHB in biological specimens is lacking, due to challenges of separating the structural isomers and enantiomers of βOHB, and to the chemical instability of AcAc. Here we present a single UPLC-MS/MS method that simultaneously quantifies both AcAc and βOHB using independent stable isotope internal standards for both ketones. This method incorporates one sample preparation step requiring only 7 min of analysis per sample. The output is linear over three orders of magnitude, shows very low limits of detection and quantification, is highly specific, and shows favorable recovery yields from mammalian serum and tissue samples. Tandem MS discriminates D-βOHB from structural isomers 2- or 4-hydroxybutyrate as well as 3-hydroxyisobutyrate (3-HIB). Finally, a simple derivatization distinguishes D- and L-enantiomers of βOHB, 3-HIB, and 2-OHB, using the same rapid chromatographic platform. Together, this simple, efficient, reproducible, scalable, and all-encompassing method will support basic and clinical research laboratories interrogating ketone metabolism and redox biochemistry.
(Published by Elsevier B.V.)
References: Anal Chem. 2013 Jul 16;85(14):6876-84. (PMID: 23781873)
Cell Metab. 2019 Feb 5;29(2):383-398.e7. (PMID: 30449686)
Diabetes Metab Rev. 1989 May;5(3):247-70. (PMID: 2656155)
Anal Bioanal Chem. 2012 Oct;404(6-7):1925-34. (PMID: 22895741)
Life Sci. 2006 Feb 16;78(12):1385-91. (PMID: 16225892)
Cell Metab. 2017 Feb 7;25(2):262-284. (PMID: 28178565)
J Biol Chem. 1977 Aug 10;252(15):5222-6. (PMID: 885847)
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Feb 15;885-886:37-42. (PMID: 22226469)
J Clin Invest. 2014 Dec;124(12):5175-90. (PMID: 25347470)
J Biol Chem. 1975 Aug 10;250(15):5774-61. (PMID: 1171099)
J Biol Chem. 2011 Mar 4;286(9):6902-10. (PMID: 21209089)
Am J Physiol Endocrinol Metab. 2010 Jun;298(6):E1226-35. (PMID: 20233938)
Nature. 2018 Aug;560(7719):499-503. (PMID: 30051890)
Annu Rev Nutr. 2006;26:1-22. (PMID: 16848698)
S Afr Med J. 1980 Jan 26;57(4):125-7. (PMID: 7404121)
Clin Chem. 1993 May;39(5):920. (PMID: 8485903)
J Clin Invest. 1966 Nov;45(11):1751-69. (PMID: 5926444)
Science. 2013 Jan 11;339(6116):211-4. (PMID: 23223453)
J Physiol. 1980 Apr;301:79-90. (PMID: 6997456)
J Biol Chem. 2011 Dec 16;286(50):42992-3003. (PMID: 22016388)
Forensic Sci Int. 1994 Feb;64(2-3):83-8. (PMID: 8175092)
J Chromatogr A. 2017 Mar 3;1487:194-200. (PMID: 28139228)
Annu Rev Biochem. 1980;49:395-420. (PMID: 6157353)
J Anal Toxicol. 2009 Oct;33(8):502-7. (PMID: 19874659)
Mol Cell. 2016 Apr 21;62(2):194-206. (PMID: 27105115)
Proc Natl Acad Sci U S A. 2020 Mar 17;117(11):6170-6177. (PMID: 32127481)
Clin Biochem. 2013 Dec;46(18):1877-83. (PMID: 23994603)
Nat Methods. 2018 Sep;15(9):681-684. (PMID: 30150755)
Clin Chem. 1985 Apr;31(4):596-8. (PMID: 3978793)
Clin Chim Acta. 2014 Jun 10;433:278-83. (PMID: 24721643)
Nat Med. 2015 Mar;21(3):263-9. (PMID: 25686106)
J Autom Methods Manag Chem. 2001;23(3):69-76. (PMID: 18924878)
Nature. 2020 Jul;583(7817):620-624. (PMID: 32669709)
Clin Chim Acta. 1970 May;28(2):359-64. (PMID: 5447412)
Front Physiol. 2017 Oct 30;8:848. (PMID: 29163194)
BMC Cardiovasc Disord. 2017 Nov 16;17(1):280. (PMID: 29145814)
Physiol Rev. 1980 Jan;60(1):143-87. (PMID: 6986618)
Am J Cardiol. 1995 Nov 15;76(14):1076-8. (PMID: 7484868)
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 1;879(29):3331-6. (PMID: 21856256)
Nucleic Acids Res. 2018 Jan 4;46(D1):D608-D617. (PMID: 29140435)
PLoS One. 2010 May 28;5(5):e10883. (PMID: 20526369)
J Chromatogr Sci. 1985 Mar;23(3):120-3. (PMID: 3980662)
J Biol Chem. 1960 Aug;235:2450-5. (PMID: 14415394)
Am J Clin Nutr. 2002 Jul;76(1):65-70. (PMID: 12081817)
Anal Biochem. 1988 Aug 15;173(1):96-105. (PMID: 3189805)
N Engl J Med. 2019 Dec 26;381(26):2541-2551. (PMID: 31881139)
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Apr 15;1083:137-145. (PMID: 29547803)
Biochem J. 1967 May;103(2):514-27. (PMID: 4291787)
Clin Lab. 2001;47(7-8):399-403. (PMID: 11499803)
Clin Chem. 1990 Jan;36(1):160-1. (PMID: 2297915)
Am J Clin Pathol. 1983 Sep;80(3):375-80. (PMID: 6410906)
Clin Chem. 1977 Oct;23(10):1893-7. (PMID: 20246)
Biochem J. 1983 Sep 15;214(3):937-42. (PMID: 6626164)
Anal Chim Acta. 2016 Oct 26;942:50-57. (PMID: 27720121)
معلومات مُعتمدة: R01 AG069781 United States AG NIA NIH HHS; R01 DK091538 United States DK NIDDK NIH HHS
فهرسة مساهمة: Keywords: Acetoacetate instability; Acetoacetate internal standard; D-beta-hydroxybutyrate structural and stereo-isomers; Quantification of ketone bodies; UPLC-MS/MS
المشرفين على المادة: 0 (Ketone Bodies)
TZP1275679 (3-Hydroxybutyric Acid)
تواريخ الأحداث: Date Created: 20210217 Date Completed: 20210514 Latest Revision: 20220402
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8783292
DOI: 10.1016/j.talanta.2020.122048
PMID: 33592770
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-3573
DOI:10.1016/j.talanta.2020.122048