دورية أكاديمية
Quantifying Absolute Neutralization Titers against SARS-CoV-2 by a Standardized Virus Neutralization Assay Allows for Cross-Cohort Comparisons of COVID-19 Sera.
العنوان: | Quantifying Absolute Neutralization Titers against SARS-CoV-2 by a Standardized Virus Neutralization Assay Allows for Cross-Cohort Comparisons of COVID-19 Sera. |
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المؤلفون: | Oguntuyo KY; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Stevens CS; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Hung CT; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Ikegame S; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Acklin JA; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Kowdle SS; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Carmichael JC; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Chiu HP; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Azarm KD; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Haas GD; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Amanat F; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Klingler J; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.; James J. Peters VA Medical Center, Bronx, New York, USA., Baine I; Department of Pathology, Molecular, and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Arinsburg S; Department of Pathology, Molecular, and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Bandres JC; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.; James J. Peters VA Medical Center, Bronx, New York, USA., Siddiquey MNA; Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA., Schilke RM; Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA., Woolard MD; Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA., Zhang H; Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA., Duty AJ; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Kraus TA; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Moran TM; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Tortorella D; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Lim JK; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Gamarnik AV; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.; COVIDAR Argentina Consortium, Buenos Aires, Argentina., Hioe CE; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.; James J. Peters VA Medical Center, Bronx, New York, USA., Zolla-Pazner S; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Ivanov SS; Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA., Kamil JP; Department of Microbiology and Immunology, Louisiana State University Health Science Center Shreveport, Shreveport, Louisiana, USA., Krammer F; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA., Lee B; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA Benhur.Lee@mssm.edu. |
مؤلفون مشاركون: | COVIDAR Argentina Consortium |
المصدر: | MBio [mBio] 2021 Feb 16; Vol. 12 (1). Date of Electronic Publication: 2021 Feb 16. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101519231 Publication Model: Electronic Cited Medium: Internet ISSN: 2150-7511 (Electronic) NLM ISO Abbreviation: mBio Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, D.C. : American Society for Microbiology |
مواضيع طبية MeSH: | COVID-19/*diagnosis , COVID-19/*immunology , SARS-CoV-2/*immunology , SARS-CoV-2/*pathogenicity, Antibodies, Neutralizing/immunology ; Antibodies, Neutralizing/metabolism ; Antibodies, Viral/metabolism ; Enzyme-Linked Immunosorbent Assay ; Humans ; Neutralization Tests |
مستخلص: | The global coronavirus disease 2019 (COVID-19) pandemic has mobilized efforts to develop vaccines and antibody-based therapeutics, including convalescent-phase plasma therapy, that inhibit viral entry by inducing or transferring neutralizing antibodies (nAbs) against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein (CoV2-S). However, rigorous efficacy testing requires extensive screening with live virus under onerous biosafety level 3 (BSL3) conditions, which limits high-throughput screening of patient and vaccine sera. Myriad BSL2-compatible surrogate virus neutralization assays (VNAs) have been developed to overcome this barrier. Yet, there is marked variability between VNAs and how their results are presented, making intergroup comparisons difficult. To address these limitations, we developed a standardized VNA using CoV2-S pseudotyped particles (CoV2pp) based on vesicular stomatitis virus bearing the Renilla luciferase gene in place of its G glycoprotein (VSVΔG); this assay can be robustly produced at scale and generate accurate neutralizing titers within 18 h postinfection. Our standardized CoV2pp VNA showed a strong positive correlation with CoV2-S enzyme-linked immunosorbent assay (ELISA) results and live-virus neutralizations in confirmed convalescent-patient sera. Three independent groups subsequently validated our standardized CoV2pp VNA ( n > 120). Our data (i) show that absolute 50% inhibitory concentration (absIC (Copyright © 2021 Oguntuyo et al.) |
التعليقات: | Update of: medRxiv. 2020 Aug 27;:. (PMID: 32817961) |
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معلومات مُعتمدة: | T32 AI007647 United States AI NIAID NIH HHS; R01 AI123449 United States AI NIAID NIH HHS; HHSN272201400008C United States AI NIAID NIH HHS; UL1 TR001433 United States TR NCATS NIH HHS; R21 AI148033 United States AI NIAID NIH HHS; I01 BX003860 United States BX BLRD VA; R01 AI116851 United States AI NIAID NIH HHS; IK6 BX004607 United States BX BLRD VA; R21 AI149033 United States AI NIAID NIH HHS; R01 AI143839 United States AI NIAID NIH HHS; R01 AI125536 United States AI NIAID NIH HHS; 75N93019C00051 United States AI NIAID NIH HHS; R01 AI139290 United States AI NIAID NIH HHS; I01 BX005794 United States BX BLRD VA; F31 AI154739 United States AI NIAID NIH HHS |
فهرسة مساهمة: | Keywords: COVID-19; SARS-CoV-2; convalescent-phase plasma; neutralizing antibodies; viral neutralization assay |
المشرفين على المادة: | 0 (Antibodies, Neutralizing) 0 (Antibodies, Viral) |
تواريخ الأحداث: | Date Created: 20210217 Date Completed: 20210301 Latest Revision: 20231107 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC8545089 |
DOI: | 10.1128/mBio.02492-20 |
PMID: | 33593976 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2150-7511 |
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DOI: | 10.1128/mBio.02492-20 |