دورية أكاديمية
Genomic investigation of atypical malaria cases in Kanel, northern Senegal.
| العنوان: | Genomic investigation of atypical malaria cases in Kanel, northern Senegal. |
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| المؤلفون: | Sy M; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal. symouhamad92@gmail.com., Badiane AS; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal., Deme AB; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal., Gaye A; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal., Ndiaye T; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal., Fall FB; Senegal National Malaria Control Programme, Dakar, Senegal., Siddle KJ; The Broad Institute of MIT and Harvard, Cambridge, MA, USA., Dieye B; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal., Ndiaye YD; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal., Diallo MA; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal., Diongue K; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal., Seck MC; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal., Ndiaye IM; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal., Cissé M; Senegal National Malaria Control Programme, Dakar, Senegal., Gueye AB; Senegal National Malaria Control Programme, Dakar, Senegal., Sène D; Senegal National Malaria Control Programme, Dakar, Senegal., Dieye Y; Malaria Control and Evaluation Partnership in Africa PATH-MACEPA, Dakar, Senegal., Souané T; Malaria Control and Evaluation Partnership in Africa PATH-MACEPA, Dakar, Senegal., MacInnis B; The Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA, USA., Volkman SK; The Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA, USA.; College of Natural, Behavioral, and Health Sciences, Simmons University, Boston, MA, USA., Wirth DF; The Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA, USA., Ndiaye D; Laboratory of Parasitology and Mycology, Aristide le Dantec Hospital, Cheikh Anta Diop University, Dakar, Senegal. |
| المصدر: | Malaria journal [Malar J] 2021 Feb 19; Vol. 20 (1), pp. 103. Date of Electronic Publication: 2021 Feb 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101139802 Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2875 (Electronic) Linking ISSN: 14752875 NLM ISO Abbreviation: Malar J Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central, [2002- |
| مواضيع طبية MeSH: | Genome, Protozoan*, Malaria, Falciparum/*classification , Plasmodium falciparum/*genetics, Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Malaria, Falciparum/diagnosis ; Malaria, Falciparum/parasitology ; Male ; Senegal ; Young Adult |
| مستخلص: | Background: The diagnosis of malaria cases in regions where the malaria burden has decreased significantly and prevalence is very low is more challenging, in part because of reduced clinical presumption of malaria. The appearance of a cluster of malaria cases with atypical symptoms in Mbounguiel, a village in northern Senegal where malaria transmission is low, in September 2018 exemplifies this scenario. The collaboration between the National Malaria Control Programme (NMCP) at the Senegal Ministry of Health and the Laboratory of Parasitology and Mycology at Cheikh Anta Diop University worked together to evaluate this cluster of malaria cases using molecular and serological tools. Methods: Malaria cases were diagnosed primarily by rapid diagnostic test (RDT), and confirmed by photo-induced electron transfer-polymerase chain reaction (PET-PCR). 24 single nucleotide polymorphisms (SNPs) barcoding was used for Plasmodium falciparum genotyping. Unbiased metagenomic sequencing and Luminex-based multi-pathogen antibody and antigen profiling were used to assess exposure to other pathogens. Results: Nine patients, of 15 suspected cases, were evaluated, and all nine samples were found to be positive for P. falciparum only. The 24 SNPs molecular barcode showed the predominance of polygenomic infections, with identifiable strains being different from one another. All patients tested positive for the P. falciparum antigens. No other pathogenic infection was detected by either the serological panel or metagenomic sequencing. Conclusions: This work, undertaken locally within Senegal as a collaboration between the NMCP and a research laboratory at University of Cheikh Anta Diop (UCAD) revealed that a cluster of malaria cases were caused by different strains of P. falciparum. The public health response in real time demonstrates the value of local molecular and genomics capacity in affected countries for disease control and elimination. |
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| معلومات مُعتمدة: | INV-004036 United States GATES Bill & Melinda Gates Foundation; MR/P028071/1 United Kingdom MRC_ Medical Research Council; R21 AI141843 United States AI NIAID NIH HHS; INV-004036 United States GATES Bill & Melinda Gates Foundation; U54 HG007480 United States HG NHGRI NIH HHS |
| فهرسة مساهمة: | Keywords: Genetic surveillance; Infectious disease; Investigation; Metagenomic sequencing; Plasmodium falciparum; Serology |
| تواريخ الأحداث: | Date Created: 20210220 Date Completed: 20210813 Latest Revision: 20240502 |
| رمز التحديث: | 20240502 |
| مُعرف محوري في PubMed: | PMC7893743 |
| DOI: | 10.1186/s12936-021-03637-x |
| PMID: | 33608006 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1475-2875 |
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| DOI: | 10.1186/s12936-021-03637-x |