دورية أكاديمية
Bacillus Toyonensis BCT-7112 T Spores as Parenteral Adjuvant of BoHV-5 Vaccine in a Murine Model.
| العنوان: | Bacillus Toyonensis BCT-7112 T Spores as Parenteral Adjuvant of BoHV-5 Vaccine in a Murine Model. |
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| المؤلفون: | Santos FDS; Biotechnology Department, Center for Technological Development, Federal University of Pelotas, Capão do Leão, Rio Grande do Sul, 96160-900, Brazil., Maubrigades LR; Biotechnology Department, Center for Technological Development, Federal University of Pelotas, Capão do Leão, Rio Grande do Sul, 96160-900, Brazil., Gonçalves VS; Biotechnology Department, Center for Technological Development, Federal University of Pelotas, Capão do Leão, Rio Grande do Sul, 96160-900, Brazil., Franz HC; Veterinary School, Federal University of Pelotas, Capão do Leão, Rio Grande do Sul, 96160-900, Brazil., Rodrigues PRC; Veterinary School, Federal University of Pelotas, Capão do Leão, Rio Grande do Sul, 96160-900, Brazil., Cunha RC; Biotechnology Department, Center for Technological Development, Federal University of Pelotas, Capão do Leão, Rio Grande do Sul, 96160-900, Brazil.; Veterinary School, Federal University of Pelotas, Capão do Leão, Rio Grande do Sul, 96160-900, Brazil., Leite FPL; Biotechnology Department, Center for Technological Development, Federal University of Pelotas, Capão do Leão, Rio Grande do Sul, 96160-900, Brazil. fleivasleite@gmail.com. |
| المصدر: | Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2021 Jun; Vol. 13 (3), pp. 655-663. Date of Electronic Publication: 2021 Feb 19. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: United States NLM ID: 101484100 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1867-1314 (Electronic) Linking ISSN: 18671306 NLM ISO Abbreviation: Probiotics Antimicrob Proteins Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY. : Springer |
| مواضيع طبية MeSH: | Adjuvants, Immunologic* , Bacillus*/immunology, Herpesviridae Infections/*prevention & control , Viral Vaccines/*immunology, Animals ; Disease Models, Animal ; Herpesvirus 5, Bovine ; Interleukin-12 ; Interleukin-4 ; Mice ; Oligopeptides ; Spores, Bacterial/immunology |
| مستخلص: | Bacterial spores of the genus Bacillus are being evaluated as adjuvant molecules capable of improving the immune response to vaccines. In this study, we investigate whether subcutaneously administered spores of B. toyonensis BCT-7112 T could enhance a vaccine immune response in mice. Three groups of mice were subcutaneously vaccinated on day 0 and received a booster on day 21 of the experiment, with the following vaccine formulations: 40 µg of recombinant glycoprotein D (rgD) from bovine herpesvirus type 5 (BoHV-5) adsorbed in 10% aluminum hydroxide (alum) without B. toyonensis spores (group 1) and B. toyonensis (1 × 10 6 viable spores) + 40 µg of rgD adsorbed in 10% alum (group 2); and B. toyonensis (1 × 10 6 viable spores) without rgD (group 3). Group 2 showed significantly higher titers (P < 0.05) of total specific serum IgG, IgG2a, and neutralizing antibodies, when compared with the groups 1 and 3. A significantly higher (P < 0.05) transcription level of cytokines IL-4, IL-12, and IFN-γ was observed in splenocytes from mice that received the B. toyonensis spores in the vaccine formulation. In addition, stimulation of the macrophage-like cell line RAW264.7 with spores of B. toyonensis markedly enhanced the cell proliferation and mRNA transcription levels of IL-4, and IL-12 cytokines in these cells. Our findings indicated that the subcutaneous administration of B. toyonensis BCT-7112 T spores enhanced the humoral and cellular immune response against BoHV-5 in mice. |
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| فهرسة مساهمة: | Keywords: Bacillus; Cytokines; Immunomodulation; Spore; Vaccine |
| المشرفين على المادة: | 0 (Adjuvants, Immunologic) 0 (Oligopeptides) 0 (Viral Vaccines) 187348-17-0 (Interleukin-12) 207137-56-2 (Interleukin-4) |
| SCR Organism: | Bacillus toyonensis |
| تواريخ الأحداث: | Date Created: 20210220 Date Completed: 20211229 Latest Revision: 20211229 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/s12602-021-09753-z |
| PMID: | 33608827 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1867-1314 |
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| DOI: | 10.1007/s12602-021-09753-z |