دورية أكاديمية
Detection of deletions in 1q25, 1p36 and 1pTEL and chromosome 17 aneuploidy in oral epithelial dysplasia and oral squamous cell carcinoma by fluorescence in situ hybridization (FISH).
| العنوان: | Detection of deletions in 1q25, 1p36 and 1pTEL and chromosome 17 aneuploidy in oral epithelial dysplasia and oral squamous cell carcinoma by fluorescence in situ hybridization (FISH). |
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| المؤلفون: | Barem Rabenhorst SH; Department of Pathology and Forensic Medicine, Molecular Genetics Laboratory (LABGEM), School of Medicine, Federal University of Ceara, Fortaleza, Brazil., Lima Verde Osterne R; Department of Pathology, University of Fortaleza / Universidade de Fortaleza, School of Medicine, Fortaleza, Brazil. Electronic address: rlimaverde@unifor.br., Weege Nonaka CF; State University of Paraíba (UEPB), Campina Grande, Paraíba, Brazil., Montezuma Sales Rodrigues A; University of Fortaleza / Universidade de Fortaleza, School of Dentistry, Fortaleza, Brazil., Luiz Maia Nogueira R; Department of Dental Clinic, Discipline of Oral and Maxillofacial Surgery and Stomatology, Federal University of Ceara School of Dentistry, Fortaleza, Brazil. Oral and Maxillofacial Surgeon, Department of Oral and Maxillofacial Surgery, Memorial Batista Hospital, Fortaleza, Brazil., Mário Rodriguez Burbano R; Research Center on Oncology, Institute of Health Sciences, Federal University of Pará, Mundurucus Street, 66073-000 Belém, Pará, Brazil., Barroso Cavalcante R; Department of Oral Pathology, University of Fortaleza / Universidade de Fortaleza, School of Dentistry, Fortaleza, Brazil. |
| المصدر: | Oral oncology [Oral Oncol] 2021 May; Vol. 116, pp. 105221. Date of Electronic Publication: 2021 Feb 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 9709118 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0593 (Electronic) Linking ISSN: 13688375 NLM ISO Abbreviation: Oral Oncol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Oxford ; New York : Pergamon, c1997- |
| مواضيع طبية MeSH: | Chromosomes, Human, Pair 17* , Mouth Mucosa*/pathology , Mouth Neoplasms*/genetics , Squamous Cell Carcinoma of Head and Neck*/genetics, Aneuploidy ; Gene Deletion ; Humans ; Hyperplasia ; In Situ Hybridization, Fluorescence |
| مستخلص: | Objective: To identify chromosome deletions in 1q25, 1p36 and 1pTEL, and chromosome 17 ploidy status in oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC). Material and Methods: Samples from 57 OED and 63 OSCC were selected. FISH was performed using centromeric probes 17 and n LSIR 1p36/LSI 1q25 Dual Color Probe. Results: In OED, deletions were found only in 1pTEL region (29.8%). In OSCC, there was a higher frequency of deletion in 1pTEL (79.4%), followed by 1p36 (73.0%), and 1q25 (20.6%). Advanced TNM clinical stages (III/IV) showed all the deletions studied; at early clinical stages (I/II) of OSCC, deletions were observed only in 1pTEL. The frequency of deletion in 1p36 was 17.0 times higher in OSCC at advanced clinical stages (PR: 17.00). The median number of cell nuclei with chromosome 17 aneuploidy was higher in OSCC than in OED (P < 0.001). Early clinical stages of OSCC showed lower median number nuclei with aneuploidy when compared to advanced tumors (P < 0.05). Tumors harboring deletions in 1p36, 1q25 and 1pTEL revealed higher median numbers of trisomic/polysomic nuclei when compared to lesions exhibiting no abnormalities in chromosome 1 (P < 0.05). Conclusion: A higher prevalence of chromosomal abnormalities was found in OSCC than in OED, while in OSCC, higher abnormalities were present in lesions with higher TNM staging. 1pTEL deletion and monosomy of chromosome 17 are possible markers for progression of OED to OSCC. 1p36 deletion and trisomy/polysomy of chromosome 17 could be markers of worse prognosis of OSCC. (Copyright © 2021 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Chromosome 1; Chromosome 17; Chromosome deletion; Mouth neoplasms; Squamous cell carcinoma |
| تواريخ الأحداث: | Date Created: 20210220 Date Completed: 20220131 Latest Revision: 20220131 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.oraloncology.2021.105221 |
| PMID: | 33610067 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1879-0593 |
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| DOI: | 10.1016/j.oraloncology.2021.105221 |