دورية أكاديمية
Ex vivo model of human skin (hOSEC) for assessing the dermatokinetics of the anti-melanoma drug Dacarbazine.
| العنوان: | Ex vivo model of human skin (hOSEC) for assessing the dermatokinetics of the anti-melanoma drug Dacarbazine. |
|---|---|
| المؤلفون: | Leite MN; Division of Dermatology - Wound Healing & Hansen's Disease Lab, Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: marcelnani@usp.br., Viegas JSR; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: julianarosa@usp.br., Praça FSG; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: fgarcia@fcfrp.usp.br., de Paula NA; Division of Dermatology - Wound Healing & Hansen's Disease Lab, Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: npbiomed@yahoo.com.br., Ramalho LNZ; Department of Pathology and Legal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: lramalho@fmrp.usp.br., Bentley MVLB; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: vbentley@usp.br., Frade MAC; Division of Dermatology - Wound Healing & Hansen's Disease Lab, Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: mandrey@fmrp.usp.br. |
| المصدر: | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences [Eur J Pharm Sci] 2021 May 01; Vol. 160, pp. 105769. Date of Electronic Publication: 2021 Feb 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science B.V Country of Publication: Netherlands NLM ID: 9317982 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0720 (Electronic) Linking ISSN: 09280987 NLM ISO Abbreviation: Eur J Pharm Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science B.V Original Publication: Amsterdam ; New York : Elsevier, c1993- |
| مواضيع طبية MeSH: | Dacarbazine* , Pharmaceutical Preparations*, Animals ; Fibroblasts ; Humans ; Keratinocytes ; Skin |
| مستخلص: | Alternative models to replace animals in experimental studies remain a challenge in testing the effectiveness of dermatologic and cosmetic drugs. We proposed a model of human organotypic skin explant culture (hOSEC) to assess the profile of cutaneous drug skin distribution, adopting dacarbazine as a model, and respective new methodologies for dermatokinetic analysis. The viability tests were evaluated in primary keratinocytes and fibroblasts, and skin by MTT and TTC assays, respectively. Then, dacarbazine was applied to the culture medium, and the hOSEC method was applied to verify the dynamics of skin distribution of dacarbazine and determine its dermatokinetic profile. The results of cell and tissue viability showed that both were considered viable. The dermatokinetic results indicated that dacarbazine can be absorbed through the skin, reaching a concentration of 36.36 µg/mL (18,18%) of the initial dose (200 µg/mL) after 12 h in culture. Histological data showed that the skin maintained its structure throughout the tested time that the hOSEC method was applied. No apoptotic cells were observed in the epidermal and dermal layers. No visible changes in the dermo-epidermal junction and no inflammatory processes with the recruitment of defense cells were observed. Hence, these findings suggest that the hOSEC concept as an alternative ex vivo model for assessing the dynamics of skin distribution of drugs, such as dacarbazine, and determining their respective dermatokinetic profiles. (Copyright © 2021. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Alternative models; Dacarbazine; Dermatokinetic profile; Skin distribution of drugs; hOSEC model |
| المشرفين على المادة: | 0 (Pharmaceutical Preparations) 7GR28W0FJI (Dacarbazine) |
| تواريخ الأحداث: | Date Created: 20210221 Date Completed: 20210621 Latest Revision: 20210621 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.ejps.2021.105769 |
| PMID: | 33610737 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0720 |
|---|---|
| DOI: | 10.1016/j.ejps.2021.105769 |