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Genetic diversity assessed using PFGE, MLP and MLST in Candida spp. candidemia isolates obtained from a Brazilian hospital.

التفاصيل البيبلوغرافية
العنوان: Genetic diversity assessed using PFGE, MLP and MLST in Candida spp. candidemia isolates obtained from a Brazilian hospital.
المؤلفون: Canela HMS; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Bloco S - Térreo - Sala 013A-S, Avenida do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil., Cardoso B; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Bloco S - Térreo - Sala 013A-S, Avenida do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil., Frazão MR; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Bloco S - Térreo - Sala 013A-S, Avenida do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil., Falcão JP; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Bloco S - Térreo - Sala 013A-S, Avenida do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil., Vitali LH; Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil., Martinez R; Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil., da Silva Ferreira ME; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Bloco S - Térreo - Sala 013A-S, Avenida do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil. mesfe@fcfrp.usp.br.
المصدر: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2021 Jun; Vol. 52 (2), pp. 503-516. Date of Electronic Publication: 2021 Feb 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2019- : Switzerland, AG : Springer International Publishing
Original Publication: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia
مواضيع طبية MeSH: Genetic Variation*, Candida/*genetics , Candida/*isolation & purification , Candidemia/*microbiology, Adolescent ; Adult ; Aged ; Aged, 80 and over ; Brazil ; Candida/classification ; Candidemia/blood ; Child ; Child, Preschool ; Electrophoresis, Gel, Pulsed-Field ; Female ; Hospitals/statistics & numerical data ; Humans ; Infant ; Infant, Newborn ; Male ; Middle Aged ; Multilocus Sequence Typing ; Mycological Typing Techniques ; Phylogeny ; Young Adult
مستخلص: Candida spp. are the main causative agents of invasive fungal infections in immunocompromised patients. Candidemia has attributable mortality rates of 15 to 35% and increases hospitalisation time and costs, thus making this disease a public health concern. This study aimed to use pulsed-field gel electrophoresis (PFGE), microsatellite length polymorphism (MLP) and multilocus sequence typing (MLST) to analyse the genetic relationships among 65 Candida spp. bloodstream isolates, including 35 Candida albicans, 15 Candida glabrata and 15 Candida tropicalis isolates, all of which were obtained from patients in a Brazilian hospital. Moreover, patient clinical data were assessed. All techniques resulted in high discriminatory indexes. C. albicans and C. tropicalis isolates showed high genetic variability, while C. glabrata isolates had relatively low genetic variability. Moreover, a cluster of C. glabrata isolates was identified in a hospital unit. New MLST sequence types, diploid sequence types and alleles are described. Relationships were not observed between the molecular typing results and clinical characteristics. The molecular typing of clinical strains increases our understanding of candidemia epidemiology and promotes the development of strategies that can reduce the incidence of this disease. Moreover, this study is the first to combine these techniques to genotype these three species in Brazil.
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معلومات مُعتمدة: 2014/10628-0 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2013/23485-0 Fundação de Amparo à Pesquisa do Estado de São Paulo
فهرسة مساهمة: Keywords: Candida spp.; Candidemia; Microsatellite length polymorphism; Multilocus sequence typing; Pulsed-field gel electrophoresis
تواريخ الأحداث: Date Created: 20210221 Date Completed: 20211007 Latest Revision: 20240402
رمز التحديث: 20240402
مُعرف محوري في PubMed: PMC8105495
DOI: 10.1007/s42770-021-00446-x
PMID: 33611738
قاعدة البيانات: MEDLINE
الوصف
تدمد:1678-4405
DOI:10.1007/s42770-021-00446-x