دورية أكاديمية
Megasphaera lornae sp. nov., Megasphaera hutchinsoni sp. nov., and Megasphaera vaginalis sp. nov.: novel bacteria isolated from the female genital tract.
| العنوان: | Megasphaera lornae sp. nov., Megasphaera hutchinsoni sp. nov., and Megasphaera vaginalis sp. nov.: novel bacteria isolated from the female genital tract. |
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| المؤلفون: | Srinivasan S; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA., Beamer MA; Magee-Womens Research Institute, Pittsburgh, PA, USA., Fiedler TL; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA., Austin MN; Magee-Womens Research Institute, Pittsburgh, PA, USA., Sizova MV; Present address: Evelo Biosciences, 620 Memorial Drive, Cambridge, MA, USA.; Department of Biology, Northeastern University, Boston, MA, USA., Strenk SM; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA., Agnew KJ; Division of Gynecologic Oncology, Department of Obstetrics & Gynecology, University of Washington Medical Center, Seattle, WA, USA., Gowda GAN; Northwest Metabolomics Research Center and Mitochondrial and Metabolism Center, Anesthesiology and Pain Medicine, University of Washington Medical Center, Seattle, WA, USA., Raftery D; Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.; Northwest Metabolomics Research Center and Mitochondrial and Metabolism Center, Anesthesiology and Pain Medicine, University of Washington Medical Center, Seattle, WA, USA., Epstein SS; Department of Biology, Northeastern University, Boston, MA, USA., Fredricks DN; Department of Medicine, University of Washington, Seattle, WA, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA., Hillier SL; University of Pittsburgh School of Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, Pittsburgh, PA, USA.; Magee-Womens Research Institute, Pittsburgh, PA, USA. |
| المصدر: | International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2019 Jun; Vol. 71 (3). Date of Electronic Publication: 2021 Feb 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 100899600 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1466-5034 (Electronic) Linking ISSN: 14665026 NLM ISO Abbreviation: Int J Syst Evol Microbiol Subsets: PubMed not MEDLINE; MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: Reading, UK : Society for General Microbiology, c2000- |
| مستخلص: | Six strictly anaerobic Gram-negative bacteria representing three novel species were isolated from the female reproductive tract. The proposed type strains for each species were designated UPII 199-6 T , KA00182 T and BV3C16-1 T . Phylogenetic analyses based on 16S rRNA gene sequencing indicated that the bacterial isolates were members of the genus Megasphaera . UPII 199-6 T and KA00182 T had 16S rRNA gene sequence identities of 99.9 % with 16S rRNA clone sequences previously amplified from the human vagina designated as Megasphaera type 1 and Megasphaera type 2, members of the human vaginal microbiota associated with bacterial vaginosis, preterm birth and HIV acquisition. UPII 199-6 T exhibited sequence identities ranging from 92.9 to 93.6 % with validly named Megasphaera isolates and KA00182 T had 16S rRNA gene sequence identities ranging from 92.6-94.2 %. BV3C16-1 T was most closely related to Megasphaera cerevisiae with a 16S rRNA gene sequence identity of 95.4 %. Cells were coccoid or diplococcoid, non-motile and did not form spores. Genital tract isolates metabolized organic acids but were asaccharolytic. The isolates also metabolized amino acids. The DNA G+C content for the genome sequences of UPII 199-6 T , KA00182 T and BV3C16-1 T were 46.4, 38.9 and 49.8 mol%, respectively. Digital DNA-DNA hybridization and average nucleotide identity between the genital tract isolates and other validly named Megasphaera species suggest that each isolate type represents a new species. The major fatty acid methyl esters include the following: C |
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| معلومات مُعتمدة: | R01 HG005816 United States HG NHGRI NIH HHS; U19 AI120249 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: Megasphaera; Negativicutes; Veillonellaceae; bacterial vaginosis; genital tract bacteria; human vagina |
| تواريخ الأحداث: | Date Created: 20210222 Latest Revision: 20210721 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8161547 |
| DOI: | 10.1099/ijsem.0.004702 |
| PMID: | 33616513 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1466-5034 |
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| DOI: | 10.1099/ijsem.0.004702 |