دورية أكاديمية
PCR-Free Methodology for Detection of Single-Nucleotide Polymorphism with a Cationic Polythiophene Reporter.
| العنوان: | PCR-Free Methodology for Detection of Single-Nucleotide Polymorphism with a Cationic Polythiophene Reporter. |
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| المؤلفون: | Yucel M; Department of Bioengineering and Biotechnology, Izmir Institute of Technology, Izmir 35430, Turkey., Koc A; Department of Medical Genetics, Faculty of Medicine, Dokuz Eylul University, Izmir 35330, Turkey., Ulgenalp A; Department of Medical Genetics, Faculty of Medicine, Dokuz Eylul University, Izmir 35330, Turkey., Akkoc GD; Department of Chemistry, Izmir Institute of Technology, İzmir 35430, Turkey., Ceyhan M; Department of Basic Oncology, Institute of Oncology, Dokuz Eylul University, Izmir 35330, Turkey., Yildiz UH; Department of Chemistry, Izmir Institute of Technology, İzmir 35430, Turkey.; Department of Photonic Science and Engineering, Izmir Institute of Technology, Izmir 35430, Turkey. |
| المصدر: | ACS sensors [ACS Sens] 2021 Mar 26; Vol. 6 (3), pp. 950-957. Date of Electronic Publication: 2021 Feb 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101669031 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-3694 (Electronic) Linking ISSN: 23793694 NLM ISO Abbreviation: ACS Sens Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Chemical Society, [2016]- |
| مواضيع طبية MeSH: | Polymers* , Thiophenes*, Humans ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide |
| مستخلص: | This study presents a nonamplification-based nucleic acid assay for the detection of single-nucleotide polymorphism (SNP) associated with familial Mediterranean fever (FMF) besides polymerase chain reaction (PCR)-based methodologies. The major objective is to show the potential of the proposed assay for rapid screening of FMF in a Mediterranean region of 400 million population. The assay relies on binding difference of specially designed wild and mutant primers to the target genomic DNA, followed by determination of unbound primers by quick titration of a cationic polythiophene reporter. The fluorescent reporter exhibits signal transition from 525 to 580 nm in the presence of unbound primers, and it correlates the binding affinity of label-free primers to the homozygous wild and mutant genomes. As a proof of concept, 26 real samples are studied relying on the ON and OFF fluorescence signals of the cationic polythiophene reporter. The results are analyzed by principal component analysis (PCA), which provides clear separation of healthy and patient individuals. The further analysis by support vector machine (SVM) classification has revealed that our assay converges to 96% overall accuracy. These results support that the PCR-free nucleic acid assay has a significant potential for rapid and cost-effective screening of familial Mediterranean fever. |
| فهرسة مساهمة: | Keywords: DNA biosensors; PCR-free SNP detection; conjugated polyelectrolyte; familial Mediterranean fever; nucleic acid assay |
| المشرفين على المادة: | 0 (Polymers) 25233-34-5 (polythiophene) 0 (Thiophenes) |
| تواريخ الأحداث: | Date Created: 20210223 Date Completed: 20221031 Latest Revision: 20221031 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1021/acssensors.0c02130 |
| PMID: | 33621051 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 2379-3694 |
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| DOI: | 10.1021/acssensors.0c02130 |