دورية أكاديمية
LncRNA TRPM2-AS promotes ovarian cancer progression and cisplatin resistance by sponging miR-138-5p to release SDC3 mRNA.
| العنوان: | LncRNA TRPM2-AS promotes ovarian cancer progression and cisplatin resistance by sponging miR-138-5p to release SDC3 mRNA. |
|---|---|
| المؤلفون: | Ding Y; Department of Obstetrics and Gynecology, Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, P.R. China., Tan X; Department of Obstetrics and Gynecology, Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, P.R. China., Abasi A; Department of Obstetrics and Gynecology, Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, P.R. China., Dai Y; Department of Obstetrics and Gynecology, Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, P.R. China., Wu R; Department of Obstetrics and Gynecology, Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, P.R. China., Zhang T; Department of Obstetrics and Gynecology, Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, P.R. China., Li K; Department of Obstetrics and Gynecology, Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, P.R. China., Yan M; Department of Obstetrics and Gynecology, Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, P.R. China., Huang X; Department of Obstetrics and Gynecology, Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, P.R. China. |
| المصدر: | Aging [Aging (Albany NY)] 2021 Feb 17; Vol. 13 (5), pp. 6832-6848. Date of Electronic Publication: 2021 Feb 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Impact Journals, LLC Country of Publication: United States NLM ID: 101508617 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1945-4589 (Electronic) Linking ISSN: 19454589 NLM ISO Abbreviation: Aging (Albany NY) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Albany, NY : Impact Journals, LLC |
| مواضيع طبية MeSH: | MicroRNAs/*metabolism , Ovarian Neoplasms/*pathology , RNA, Messenger/*metabolism , Syndecan-3/*genetics , TRPM Cation Channels/*genetics, Antineoplastic Agents/pharmacology ; Carcinogenesis/genetics ; Cisplatin/pharmacology ; Disease Progression ; Drug Resistance, Neoplasm/genetics ; Female ; Humans ; Middle Aged ; Ovarian Neoplasms/genetics ; Ovarian Neoplasms/metabolism ; RNA, Long Noncoding/metabolism ; Syndecan-3/metabolism ; TRPM Cation Channels/metabolism ; Tumor Cells, Cultured |
| مستخلص: | The role of TRPM2-AS lncRNA in OvC has not been explored. This study aimed to investigate whether and how TRPM2-AS contributes to the progression of OvC. First, qRT-PCR was employed to measure the expression of TRPM2-AS, miR-138-5p and SDC3 in OvC samples. A xenograft formation assay was subsequently performed to detect the tumor growth in vivo . The cell viability, colony formation, cell migration, cell invasion and cell apoptosis were later evaluated using a series of experiments. The western blot assay was utilized to detect the SDC3 protein expression and cell-apoptosis markers. Luciferase reporter gene assay, RIP, and RNA pull-down assays were performed to identify the association between TRPM2-AS, miR-138-5p and SDC3 . Findings indicated that the expression of TRPM2-AS and SDC3 was significantly upregulated in OvC tissues and cells, while miR-138-5p expression was significantly downregulated in OvC samples. Unlike miR-138-5p, TRPM2-AS and SDC3 were found to promote OvC development. It was also found that TRPM2-AS could sponge miR-138-5p to release SDC3 , thus promoting OvC progression. Apart from that, we discovered that both sh-TRPM2-AS and cisplatin could enhance the apoptosis of OvC cells. Overall, our findings suggested that the TRPM2-AS/miR-138-5p/ SDC3 axis was closely associated with OvC tumorigenesis and cisplatin resistance. |
| References: | Cell Death Dis. 2018 Jul 3;9(7):742. (PMID: 29970910) Mol Ther Nucleic Acids. 2019 Sep 6;17:92-101. (PMID: 31234009) Curr Cancer Drug Targets. 2020;20(6):461-470. (PMID: 32271695) Nat Rev Drug Discov. 2017 Mar;16(3):203-222. (PMID: 28209991) Cancer Biol Ther. 2019;20(6):760-773. (PMID: 30810442) Int J Biochem Cell Biol. 2020 Mar;120:105683. (PMID: 31887411) Theranostics. 2018 Oct 6;8(19):5200-5212. (PMID: 30555541) Eur J Cancer. 2013 Mar;49(4):964-73. (PMID: 23079474) Exp Ther Med. 2018 Sep;16(3):1629-1638. (PMID: 30186381) Eur Rev Med Pharmacol Sci. 2017 Jun;21(11):2626-2634. (PMID: 28678322) Annu Rev Pathol. 2014;9:287-314. (PMID: 24079833) Cell Res. 2008 Nov;18(11):1128-40. (PMID: 18957938) J Cell Biochem. 2019 Oct;120(10):16921-16933. (PMID: 31104318) Eur Rev Med Pharmacol Sci. 2018 Jul;22(14):4440-4447. (PMID: 30058681) Reprod Biol. 2018 Sep;18(3):218-224. (PMID: 30054097) Cancer Biol Ther. 2018 Jun 3;19(6):534-542. (PMID: 29565706) Biol Res. 2020 Apr 29;53(1):18. (PMID: 32349783) Taiwan J Obstet Gynecol. 2014 Dec;53(4):536-41. (PMID: 25510697) Cancer Sci. 2018 Sep;109(9):2919-2936. (PMID: 29968393) FEBS J. 2017 Jan;284(1):27-41. (PMID: 27790852) Oncogenesis. 2020 Mar 2;9(3):29. (PMID: 32123162) Theranostics. 2018 Jul 30;8(16):4279-4294. (PMID: 30214620) Nat Commun. 2019 Nov 26;10(1):5367. (PMID: 31772167) Signal Transduct Target Ther. 2016 Jan 28;1:15004. (PMID: 29263891) J Cancer Res Clin Oncol. 2019 Oct;145(10):2507-2517. (PMID: 31485766) Cancer Sci. 2018 Oct;109(10):3068-3079. (PMID: 30099826) J Cell Physiol. 2019 May;234(5):6361-6370. (PMID: 30229902) Biomed Pharmacother. 2018 May;101:536-542. (PMID: 29505924) Nat Commun. 2016 Aug 26;7:12645. (PMID: 27561551) World J Gastroenterol. 2014 Mar 21;20(11):3018-24. (PMID: 24659893) Cancer Res. 2018 Aug 1;78(15):4175-4190. (PMID: 29748371) Methods Mol Biol. 2018;1699:45-66. (PMID: 29086367) |
| فهرسة مساهمة: | Keywords: SDC3; TRPM2-AS; lncRNAs; miR-138-5p; ovarian cancer |
| المشرفين على المادة: | 0 (Antineoplastic Agents) 0 (MIRN138 microRNA, human) 0 (MicroRNAs) 0 (RNA, Long Noncoding) 0 (RNA, Messenger) 0 (SDC3 protein, human) 0 (Syndecan-3) 0 (TRPM Cation Channels) 0 (TRPM2 protein, human) Q20Q21Q62J (Cisplatin) |
| تواريخ الأحداث: | Date Created: 20210223 Date Completed: 20210726 Latest Revision: 20210726 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC7993682 |
| DOI: | 10.18632/aging.202541 |
| PMID: | 33621194 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1945-4589 |
|---|---|
| DOI: | 10.18632/aging.202541 |