دورية أكاديمية
Antibody affinity versus dengue morphology influences neutralization.
| العنوان: | Antibody affinity versus dengue morphology influences neutralization. |
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| المؤلفون: | Fibriansah G; Emerging Infectious Diseases, Duke-National University of Singapore Medical School, Singapore, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore, Singapore., Lim EXY; Emerging Infectious Diseases, Duke-National University of Singapore Medical School, Singapore, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore, Singapore., Marzinek JK; Bioinformatics Institute, A*STAR (Agency for Science, Technology and Research), Singapore, Singapore., Ng TS; Emerging Infectious Diseases, Duke-National University of Singapore Medical School, Singapore, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore, Singapore., Tan JL; Emerging Infectious Diseases, Duke-National University of Singapore Medical School, Singapore, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore, Singapore., Huber RG; Bioinformatics Institute, A*STAR (Agency for Science, Technology and Research), Singapore, Singapore., Lim XN; Emerging Infectious Diseases, Duke-National University of Singapore Medical School, Singapore, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore, Singapore., Chew VSY; Emerging Infectious Diseases, Duke-National University of Singapore Medical School, Singapore, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore, Singapore., Kostyuchenko VA; Emerging Infectious Diseases, Duke-National University of Singapore Medical School, Singapore, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore, Singapore., Shi J; Centre for BioImaging Sciences, National University of Singapore, Singapore, Singapore., Anand GS; Department of Biological Sciences, National University of Singapore, Singapore, Singapore., Bond PJ; Bioinformatics Institute, A*STAR (Agency for Science, Technology and Research), Singapore, Singapore.; Department of Biological Sciences, National University of Singapore, Singapore, Singapore., Crowe JE Jr; The Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America.; Departments of Pediatrics and Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America., Lok SM; Emerging Infectious Diseases, Duke-National University of Singapore Medical School, Singapore, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore, Singapore. |
| المصدر: | PLoS pathogens [PLoS Pathog] 2021 Feb 23; Vol. 17 (2), pp. e1009331. Date of Electronic Publication: 2021 Feb 23 (Print Publication: 2021). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science, c2005- |
| مواضيع طبية MeSH: | Antibodies, Monoclonal/*immunology , Antibodies, Neutralizing/*immunology , Antibodies, Viral/*immunology , Dengue/*immunology , Dengue Virus/*immunology , Epitopes/*immunology , Viral Envelope Proteins/*immunology, Antibodies, Monoclonal/chemistry ; Antibodies, Monoclonal/metabolism ; Antibodies, Neutralizing/chemistry ; Antibodies, Neutralizing/metabolism ; Antibodies, Viral/chemistry ; Antibodies, Viral/metabolism ; Antibody Affinity ; Antibody Specificity ; Dengue/virology ; Dengue Virus/chemistry ; Dengue Virus/metabolism ; Epitopes/metabolism ; Humans ; Molecular Dynamics Simulation ; Protein Conformation ; Serogroup |
| مستخلص: | Different strains within a dengue serotype (DENV1-4) can have smooth, or "bumpy" surface morphologies with different antigenic characteristics at average body temperature (37°C). We determined the neutralizing properties of a serotype cross-reactive human monoclonal antibody (HMAb) 1C19 for strains with differing morphologies within the DENV1 and DENV2 serotypes. We mapped the 1C19 epitope to E protein domain II by hydrogen deuterium exchange mass spectrometry, cryoEM and molecular dynamics simulations, revealing that this epitope is likely partially hidden on the virus surface. We showed the antibody has high affinity for binding to recombinant DENV1 E proteins compared to those of DENV2, consistent with its strong neutralizing activities for all DENV1 strains tested regardless of their morphologies. This finding suggests that the antibody could out-compete E-to-E interaction for binding to its epitope. In contrast, for DENV2, HMAb 1C19 can only neutralize when the epitope becomes exposed on the bumpy-surfaced particle. Although HMAb 1C19 is not a suitable therapeutic candidate, this study with HMAb 1C19 shows the importance of choosing a high-affinity antibody that could neutralize diverse dengue virus morphologies for therapeutic purposes. Competing Interests: I have read the journal’s policy and the authors of this manuscript have the following competing interests: J.E.C. has served as a consultant for Lilly and Luna Biologics, is a member of the Scientific Advisory Boards of CompuVax and Meissa Vaccines and is Founder of IDBiologics. The Crowe laboratory at Vanderbilt University Medical Center has received sponsored research agreements from IDBiologics and AstraZeneca. |
| References: | Cell. 2002 Mar 8;108(5):717-25. (PMID: 11893341) EMBO Mol Med. 2014 Mar;6(3):358-71. (PMID: 24421336) Nature. 2013 Apr 25;496(7446):504-7. (PMID: 23563266) J Virol. 2013 Jul;87(13):7585-92. (PMID: 23637405) PLoS Negl Trop Dis. 2019 Nov 1;13(11):e0007142. (PMID: 31675360) Acta Crystallogr A. 1991 Mar 1;47 ( Pt 2):110-9. (PMID: 2025413) J Virol. 2014 Jan;88(1):477-82. (PMID: 24155405) Nat Struct Mol Biol. 2013 Jan;20(1):105-10. (PMID: 23241927) Cell. 2006 Feb 10;124(3):485-93. (PMID: 16469696) J Struct Biol. 2003 Jun;142(3):334-47. (PMID: 12781660) Lancet. 2012 Nov 3;380(9853):1559-67. (PMID: 22975340) Nature. 1995 May 25;375(6529):291-8. (PMID: 7753193) J Struct Biol. 2012 Dec;180(3):519-30. (PMID: 23000701) Structure. 2012 Feb 8;20(2):303-14. (PMID: 22285214) J Comput Chem. 2005 Dec;26(16):1701-18. (PMID: 16211538) Lancet. 2014 Oct 11;384(9951):1358-65. (PMID: 25018116) J Struct Biol. 2007 Jan;157(1):38-46. (PMID: 16859925) Adv Virus Res. 2008;72:1-76. (PMID: 19081488) J Virol. 2013 Feb;87(4):2287-93. (PMID: 23236058) J Struct Biol. 1999 Dec 1;128(1):82-97. (PMID: 10600563) J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254) J Struct Biol. 2007 Oct;160(1):11-27. (PMID: 17698370) Metallomics. 2014 May;6(5):1068-78. (PMID: 24686569) Trop Med Health. 2011 Dec;39(4 Suppl):17-27. (PMID: 22500133) N Engl J Med. 2015 Jan 8;372(2):113-23. (PMID: 25365753) Sci Transl Med. 2012 Jun 20;4(139):139ra83. (PMID: 22723463) Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9. (PMID: 23569243) Nat Commun. 2020 Jun 19;11(1):3112. (PMID: 32561757) J Virol. 2013 Dec;87(23):12562-75. (PMID: 24027331) Trends Microbiol. 2016 Apr;24(4):284-293. (PMID: 26747581) Biophys J. 2012 Mar 21;102(6):1462-7. (PMID: 22455930) Curr Opin Infect Dis. 2011 Oct;24(5):442-50. (PMID: 21799408) Vaccine. 2015 Dec 10;33(50):7100-11. (PMID: 26475445) Infect Genet Evol. 2003 May;3(1):19-28. (PMID: 12797969) J Immunol. 2012 May 15;188(10):4971-9. (PMID: 22491255) Microbes Infect. 2007 Jan;9(1):1-6. (PMID: 17196419) Proteins. 1993 Dec;17(4):412-25. (PMID: 8108382) J Virol. 2006 Dec;80(23):11467-74. (PMID: 16987985) Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6986-91. (PMID: 12759475) Nat Commun. 2015 Feb 20;6:6341. (PMID: 25698059) EMBO J. 2012 May 30;31(11):2648-59. (PMID: 22543870) Structure. 2004 Sep;12(9):1607-18. (PMID: 15341726) Adv Virus Res. 2003;60:421-67. (PMID: 14689700) mBio. 2013 Nov 19;4(6):e00873-13. (PMID: 24255124) Bioinformatics. 2006 Jan 15;22(2):195-201. (PMID: 16301204) Nat Struct Mol Biol. 2008 Mar;15(3):312-7. (PMID: 18264114) Adv Virus Res. 2003;59:141-75. (PMID: 14696329) Nature. 2004 Jan 22;427(6972):313-9. (PMID: 14737159) Proc Natl Acad Sci U S A. 2012 May 8;109(19):7439-44. (PMID: 22499787) J Chem Phys. 2007 Jan 7;126(1):014101. (PMID: 17212484) J Virol. 2013 Jul;87(13):7700-7. (PMID: 23637416) |
| معلومات مُعتمدة: | U54 AI057157 United States AI NIAID NIH HHS |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Antibodies, Neutralizing) 0 (Antibodies, Viral) 0 (Epitopes) 0 (Viral Envelope Proteins) 146588-87-6 (E protein TH Sman, Dengue virus) |
| تواريخ الأحداث: | Date Created: 20210223 Date Completed: 20210623 Latest Revision: 20210623 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC7935256 |
| DOI: | 10.1371/journal.ppat.1009331 |
| PMID: | 33621239 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1553-7374 |
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| DOI: | 10.1371/journal.ppat.1009331 |