دورية أكاديمية
أعرض في EDS

The GARP Domain of the Rod CNG Channel's β1-Subunit Contains Distinct Sites for Outer Segment Targeting and Connecting to the Photoreceptor Disk Rim.

التفاصيل البيبلوغرافية
العنوان: The GARP Domain of the Rod CNG Channel's β1-Subunit Contains Distinct Sites for Outer Segment Targeting and Connecting to the Photoreceptor Disk Rim.
المؤلفون: Pearring JN; Department of Ophthalmology, University of Michigan, Ann Arbor, Michigan 48105 pearring@umich.edu.; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48105., Martínez-Márquez J; Department of Ophthalmology, University of Michigan, Ann Arbor, Michigan 48105.; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48105., Willer JR; Department of Ophthalmology, University of Michigan, Ann Arbor, Michigan 48105.; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48105., Lieu EC; Albert Eye Research Institute, Duke University, Durham, North Carolina 27710., Salinas RY; Albert Eye Research Institute, Duke University, Durham, North Carolina 27710., Arshavsky VY; Albert Eye Research Institute, Duke University, Durham, North Carolina 27710.
المصدر: The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2021 Apr 07; Vol. 41 (14), pp. 3094-3104. Date of Electronic Publication: 2021 Feb 26.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Society for Neuroscience Country of Publication: United States NLM ID: 8102140 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1529-2401 (Electronic) Linking ISSN: 02706474 NLM ISO Abbreviation: J Neurosci Subsets: MEDLINE
أسماء مطبوعة: Publication: Washington, DC : Society for Neuroscience
Original Publication: [Baltimore, Md.] : The Society, c1981-
مواضيع طبية MeSH: Cyclic Nucleotide-Gated Cation Channels/*genetics , Cyclic Nucleotide-Gated Cation Channels/*metabolism , Membrane Proteins/*genetics , Membrane Proteins/*metabolism , Nerve Tissue Proteins/*genetics , Nerve Tissue Proteins/*metabolism , Protein Domains/*physiology , Rod Cell Outer Segment/*metabolism, Animals ; Animals, Genetically Modified ; Animals, Newborn ; Binding Sites/physiology ; Cyclic Nucleotide-Gated Cation Channels/chemistry ; Female ; Male ; Membrane Proteins/chemistry ; Mice ; Mice, Knockout ; Mice, Transgenic ; Nerve Tissue Proteins/chemistry ; Rod Cell Outer Segment/chemistry ; Xenopus
مستخلص: Vision begins when light is captured by the outer segment organelle of photoreceptor cells in the retina. Outer segments are modified cilia filled with hundreds of flattened disk-shaped membranes. Disk membranes are separated from the surrounding plasma membrane, and each membrane type has unique protein components. The mechanisms underlying this protein sorting remain entirely unknown. In this study, we investigated the outer segment delivery of the rod cyclic nucleotide-gated (CNG) channel, which is located in the outer segment plasma membrane, where it mediates the electrical response to light. Using Xenopus and mouse models of both sexes, we now show that the targeted delivery of the CNG channel to the outer segment uses the conventional secretory pathway, including protein processing in both ER and Golgi, and requires preassembly of its constituent α1 and β1 subunits. We further demonstrate that the N-terminal glutamic acid-rich protein (GARP) domain of CNGβ1 contains two distinct functional regions. The glutamic acid-rich region encodes specific information targeting the channel to rod outer segments. The adjacent proline-enriched region connects the CNG channel to photoreceptor disk rims, likely through an interaction with peripherin-2. These data reveal fine functional specializations within the structural domains of the CNG channel and suggest that its sequestration to the outer segment plasma membrane requires an interaction with peripherin-2. SIGNIFICANCE STATEMENT Neurons and other differentiated cells have a remarkable ability to deliver and organize signaling proteins at precise subcellular locations. We now report that the CNG channel, mediating the electrical response to light in rod photoreceptors, contains two specialized regions within the N terminus of its β-subunit: one responsible for delivery of this channel to the ciliary outer segment organelle and another for subsequent channel sequestration into the outer segment plasma membrane. These findings expand our understanding of the molecular specializations used by neurons to populate their critical functional compartments.
(Copyright © 2021 the authors.)
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معلومات مُعتمدة: P30 EY005722 United States EY NEI NIH HHS; R01 EY030451 United States EY NEI NIH HHS; K99 EY025732 United States EY NEI NIH HHS; P30 EY007003 United States EY NEI NIH HHS; R01 EY012859 United States EY NEI NIH HHS; F31 EY007003 United States EY NEI NIH HHS; R00 EY025732 United States EY NEI NIH HHS
فهرسة مساهمة: Keywords: CNG channel; cilium; membrane trafficking; outer segment; photoreceptor
المشرفين على المادة: 0 (Cngb1 protein, mouse)
0 (Cyclic Nucleotide-Gated Cation Channels)
0 (Lrrc32 protein, mouse)
0 (Membrane Proteins)
0 (Nerve Tissue Proteins)
تواريخ الأحداث: Date Created: 20210227 Date Completed: 20210817 Latest Revision: 20211008
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8026354
DOI: 10.1523/JNEUROSCI.2609-20.2021
PMID: 33637563
قاعدة البيانات: MEDLINE
الوصف
تدمد:1529-2401
DOI:10.1523/JNEUROSCI.2609-20.2021