دورية أكاديمية
Isolation and Characterization of Live Yeast Cells from Ancient Clay Vessels.
| العنوان: | Isolation and Characterization of Live Yeast Cells from Ancient Clay Vessels. |
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| المؤلفون: | Aouizerat T; Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University of Jerusalem, Jerusalem, Israel., Maeir AM; Tell es-Safi/Gath Archaeological Project, The Martin (Szusz) Department of Land of Israel and Archaeology, Bar-Ilan University, Ramat-Gan, Israel., Paz Y; Israel Antiquities Authority, Jerusalem, Israel., Gadot Y; Tel Aviv University, Tel Aviv, Israel., Szitenberg A; Microbial Metagenomics Division, Dead Sea and Arava Science Center, Masada, Israel., Alkalay-Oren S; Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University of Jerusalem, Jerusalem, Israel., Coppenhagen-Glazer S; Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University of Jerusalem, Jerusalem, Israel., Klutstein M; Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University of Jerusalem, Jerusalem, Israel., Hazan R; Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University of Jerusalem, Jerusalem, Israel. |
| المصدر: | Bio-protocol [Bio Protoc] 2020 Jan 05; Vol. 10 (1), pp. e3473. Date of Electronic Publication: 2020 Jan 05 (Print Publication: 2020). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bio-protocol LLC Country of Publication: United States NLM ID: 101635102 Publication Model: eCollection Cited Medium: Internet ISSN: 2331-8325 (Electronic) Linking ISSN: 23318325 NLM ISO Abbreviation: Bio Protoc Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Sunnyvale, CA, USA : Bio-protocol LLC, [2011]- |
| مستخلص: | Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a novel approach based on the hypothesis that enriched yeast populations in fermented beverages could have become the dominant species in storage vessels and their descendants could be isolated and studied today. Here we present a pipeline for isolation of yeast from clay vessels uncovered in archeological sites and transferred to the microbiology lab where they can be isolated and characterized. This method opens new avenues for experimental archeology and enables attempts to recreate ancient food and beverages using the original microorganisms. Competing Interests: Competing interestsConflict of interest statement: None declared. (Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.) |
| References: | Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404) Trends Microbiol. 2016 Dec;24(12):978-990. (PMID: 27618404) Mol Ecol. 2007 May;16(10):2091-102. (PMID: 17498234) Gene. 1986;42(2):169-73. (PMID: 3015730) Lancet Infect Dis. 2004 Sep;4(9):584-92. (PMID: 15336226) J Mol Evol. 2003;57 Suppl 1:S226-32. (PMID: 15008419) Nature. 2018 Oct;562(7726):E4. (PMID: 30108364) BMC Biotechnol. 2004 Apr 21;4:8. (PMID: 15102338) mBio. 2019 Apr 30;10(2):. (PMID: 31040238) Science. 2000 Mar 24;287(5461):2196-204. (PMID: 10731133) |
| فهرسة مساهمة: | Keywords: Ancient pottery vessels; Bio-archeology; Experimental archaeology; Fermentation; Food; Yeast; beverages |
| تواريخ الأحداث: | Date Created: 20210303 Latest Revision: 20220210 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC7842698 |
| DOI: | 10.21769/BioProtoc.3473 |
| PMID: | 33654708 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2331-8325 |
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| DOI: | 10.21769/BioProtoc.3473 |