دورية أكاديمية
أعرض في EDS

Long-term effects of muscle-derived stem cell therapy on the regeneration of the urethra of female rats.

التفاصيل البيبلوغرافية
العنوان: Long-term effects of muscle-derived stem cell therapy on the regeneration of the urethra of female rats.
المؤلفون: Bilhar APM; Department of Gynecology, Universidade Federal de São Paulo, São Paulo, Brazil.; Department of Gynecology, Maternidade Escola Assis Chateaubriand, Universidade Federal do Ceará, Fortaleza, Brazil., Bortolini MAT; Department of Gynecology, Universidade Federal de São Paulo, São Paulo, Brazil. maria.augusta@gmail.com., Sé AB; Department of Gynecology, Universidade Federal de São Paulo, São Paulo, Brazil., Feitosa SM; Department of Gynecology, Universidade Federal de São Paulo, São Paulo, Brazil., Zanoteli E; Department of Neurology, Universidade de São Paulo, São Paulo, Brazil., Castro RA; Department of Gynecology, Universidade Federal de São Paulo, São Paulo, Brazil.
المصدر: International urogynecology journal [Int Urogynecol J] 2022 Apr; Vol. 33 (4), pp. 965-975. Date of Electronic Publication: 2021 Mar 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: England NLM ID: 101567041 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1433-3023 (Electronic) Linking ISSN: 09373462 NLM ISO Abbreviation: Int Urogynecol J Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Springer
مواضيع طبية MeSH: Nerve Growth Factor*/genetics , Nerve Growth Factor*/metabolism , Urethra*/injuries, Animals ; Desmin/metabolism ; Female ; Humans ; Ki-67 Antigen/metabolism ; Male ; Muscle, Skeletal ; RNA, Messenger/metabolism ; Rats ; Regeneration ; Stem Cell Transplantation
مستخلص: Introduction and Hypothesis: The aim was to analyze the long-term effects of muscle-derived stem cells (MDSCs) therapy in traumatized urethras of female rats regarding messenger ribonucleic acid (mRNA) expression of collagens 1 and 3, Ngf and Ki67; and the mRNA and protein expression of Myh11 and Myh2.
Methods: Muscle-derived stem cells were injected into the tail vein of rats 3 days after trauma by vaginal distention. Urethras were analyzed from 30 animals divided into three groups: control without injury or treatment, trauma (30 days post-injury), and MDSC (30 days post-injury who received MDSC therapy). Real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry were performed. The Kruskal-Wallis and ANOVA tests were used with p < 0.05 indicating significance.
Results: We detected increased Myh11 and Myh2 mRNA expression in the trauma group compared with the control group (p = 0.03 and p = 0.04 respectively). Ki67 and Col1a1 genes were overexpressed in the MDSC group compared with both the trauma (p = 0.02 and p = 0.008 respectively) and the control group (p = 0.01 and p = 0.03 respectively). Col3a1 gene was upregulated in the MDSC compared with the control group (p = 0.03). Ngf mRNA level was lower in the MDSC group than in the trauma group (p = 0.002). Myh11, Myh2, and Desmin proteins were overexpressed in the MDSC compared with the trauma group (1.5-fold, p = 0.01; 1.5-fold, p = 0.04; 1.3-fold, p = 0.01 respectively).
Conclusions: Muscle-derived stem cell therapy may have had long-term structural and molecular effects on the injured urethra of female rats, particularly on markers of cell proliferation, neural growth factor, extracellular matrix, and muscle content. This study suggests that MDSC therapy acted mainly to produce urethral sphincter regeneration marked by increased immunohistochemical expression of the proteins desmin, smooth muscle Myh11, and skeletal muscle Myh2.
(© 2021. The International Urogynecological Association.)
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معلومات مُعتمدة: Nº 17/2008 Conselho Nacional de Desenvolvimento Científico e Tecnológico
فهرسة مساهمة: Keywords: Adult stem cell; Muscle-derived stem cell; Regenerative medicine; Urethra; Urinary incontinence
المشرفين على المادة: 0 (Desmin)
0 (Ki-67 Antigen)
0 (RNA, Messenger)
9061-61-4 (Nerve Growth Factor)
تواريخ الأحداث: Date Created: 20210303 Date Completed: 20220422 Latest Revision: 20220520
رمز التحديث: 20221213
DOI: 10.1007/s00192-021-04708-y
PMID: 33655365
قاعدة البيانات: MEDLINE
الوصف
تدمد:1433-3023
DOI:10.1007/s00192-021-04708-y