دورية أكاديمية
A multiplexed, next generation sequencing platform for high-throughput detection of SARS-CoV-2.
العنوان: | A multiplexed, next generation sequencing platform for high-throughput detection of SARS-CoV-2. |
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المؤلفون: | Aynaud MM; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada., Hernandez JJ; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, M5S 3E1, ON, Canada.; Department of Molecular Genetics, University of Toronto, Toronto, M5S 1A8, ON, Canada., Barutcu S; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada., Braunschweig U; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, M5S 3E1, ON, Canada., Chan K; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada., Pearson JD; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada., Trcka D; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada., Prosser SL; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada., Kim J; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada., Barrios-Rodiles M; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada., Jen M; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada., Song S; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, M5S 3E1, ON, Canada.; Department of Biochemistry, University of Toronto, Toronto, M5S 1A8, ON, Canada., Shen J; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada., Bruce C; Department of Microbiology, Mount Sinai Hospital/University Health Network, Toronto, M5G 1X5, ON, Canada., Hazlett B; Department of Microbiology, Mount Sinai Hospital/University Health Network, Toronto, M5G 1X5, ON, Canada., Poutanen S; Department of Microbiology, Mount Sinai Hospital/University Health Network, Toronto, M5G 1X5, ON, Canada., Attisano L; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, M5S 3E1, ON, Canada.; Department of Biochemistry, University of Toronto, Toronto, M5S 1A8, ON, Canada., Bremner R; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada., Blencowe BJ; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, M5S 3E1, ON, Canada.; Department of Molecular Genetics, University of Toronto, Toronto, M5S 1A8, ON, Canada., Mazzulli T; Department of Microbiology, Mount Sinai Hospital/University Health Network, Toronto, M5G 1X5, ON, Canada., Han H; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, M5S 3E1, ON, Canada., Pelletier L; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada. pelletier@lunenfeld.ca.; Department of Molecular Genetics, University of Toronto, Toronto, M5S 1A8, ON, Canada. pelletier@lunenfeld.ca., Wrana JL; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, M5G 1X5, ON, Canada. wrana@lunenfeld.ca.; Department of Molecular Genetics, University of Toronto, Toronto, M5S 1A8, ON, Canada. wrana@lunenfeld.ca. |
المصدر: | Nature communications [Nat Commun] 2021 Mar 03; Vol. 12 (1), pp. 1405. Date of Electronic Publication: 2021 Mar 03. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
مواضيع طبية MeSH: | High-Throughput Nucleotide Sequencing/*methods , SARS-CoV-2/*growth & development , SARS-CoV-2/*pathogenicity, COVID-19/genetics ; COVID-19/immunology ; COVID-19/virology ; Humans ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; RNA, Viral/genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Viral Load |
مستخلص: | Population scale sweeps of viral pathogens, such as SARS-CoV-2, require high intensity testing for effective management. Here, we describe "Systematic Parallel Analysis of RNA coupled to Sequencing for Covid-19 screening" (C19-SPAR-Seq), a multiplexed, scalable, readily automated platform for SARS-CoV-2 detection that is capable of analyzing tens of thousands of patient samples in a single run. To address strict requirements for control of assay parameters and output demanded by clinical diagnostics, we employ a control-based Precision-Recall and Receiver Operator Characteristics (coPR) analysis to assign run-specific quality control metrics. C19-SPAR-Seq coupled to coPR on a trial cohort of several hundred patients performs with a specificity of 100% and sensitivity of 91% on samples with low viral loads, and a sensitivity of >95% on high viral loads associated with disease onset and peak transmissibility. This study establishes the feasibility of employing C19-SPAR-Seq for the large-scale monitoring of SARS-CoV-2 and other pathogens. |
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المشرفين على المادة: | 0 (RNA, Viral) |
تواريخ الأحداث: | Date Created: 20210304 Date Completed: 20210311 Latest Revision: 20240331 |
رمز التحديث: | 20240331 |
مُعرف محوري في PubMed: | PMC7930244 |
DOI: | 10.1038/s41467-021-21653-y |
PMID: | 33658502 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2041-1723 |
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DOI: | 10.1038/s41467-021-21653-y |