دورية أكاديمية

Quantitative levels of norovirus and hepatitis A virus in bivalve molluscs collected along the food chain in the Netherlands, 2013-2017.

التفاصيل البيبلوغرافية
العنوان: Quantitative levels of norovirus and hepatitis A virus in bivalve molluscs collected along the food chain in the Netherlands, 2013-2017.
المؤلفون: Dirks RAM; Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 WB, Wageningen, the Netherlands., Jansen CCC; Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 WB, Wageningen, the Netherlands., Hägele G; Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 WB, Wageningen, the Netherlands., Zwartkruis-Nahuis AJT; Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 WB, Wageningen, the Netherlands., Tijsma ASL; Netherlands Food and Consumer Product Safety Authority (NVWA), Catharijnesingel 59, 3511 GG, Utrecht, the Netherlands., Boxman ILA; Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 WB, Wageningen, the Netherlands. Electronic address: ingeborg.boxman@wur.nl.
المصدر: International journal of food microbiology [Int J Food Microbiol] 2021 Apr 16; Vol. 344, pp. 109089. Date of Electronic Publication: 2021 Feb 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8412849 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3460 (Electronic) Linking ISSN: 01681605 NLM ISO Abbreviation: Int J Food Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier Science Publishers, c1984-
مواضيع طبية MeSH: Caliciviridae Infections/*epidemiology , Hepatitis A/*epidemiology , Hepatitis A virus/*isolation & purification , Norovirus/*isolation & purification , Ostreidae/*virology, Animals ; Caliciviridae Infections/veterinary ; Food Chain ; Food Contamination/analysis ; Food Safety ; Hepatitis A/veterinary ; Hepatitis A virus/genetics ; Humans ; Netherlands/epidemiology ; Norovirus/genetics ; Shellfish/virology
مستخلص: Contamination of bivalve molluscs with viruses is well recognized as a food safety risk. A microbiological criterion for norovirus (NoV) and hepatitis A virus (HAV) in shellfish, however, does not exist in the European Union currently. The aim of this study was to evaluate the contamination levels of these viruses for fluctuation over a long period (2013-2017) in oyster (n = 266) and mussel samples (n = 490) using a method based on ISO/TS 15216-1: 2013. Samples were taken at different points in the food chain, either directly post-harvest, at Dutch dispatch centers or in retail stores, from September until March of each year. Altogether, 53.1% of the mussel and 31.6% of the oyster samples tested positive for NoV RNA. Simultaneous presence of NoV GI and GII RNA was observed in 31.6% of mussel and 10.2% of oyster samples. Contamination levels in NoV positive mussel samples collected post-harvest from B-areas were significantly higher than in those collected post-harvest from A-areas, or at dispatch centers or retail stores. Levels in oysters from dispatch were significantly lower than those collected in retail stores. Ready for sale mussels and oysters contained 2.04 and 1.76 mean log 10 transformed NoV genome copies/gram (gc/g), respectively. GII levels were at a constant level in ready for sale mussels throughout all sampling periods in the study. This seemed to be true for oysters as well. HAV RNA was detected in only one of the tested mussel samples (n = 392) (typed HAV 1A) and in none of the tested oyster samples (n = 228). Critical evaluation of NoV and HAV levels in shellfish can be of help for risk assessment and risk management actions.
(Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Food safety; Mussel; Oyster; Shellfish; Viral contamination
تواريخ الأحداث: Date Created: 20210304 Date Completed: 20210406 Latest Revision: 20210406
رمز التحديث: 20231215
DOI: 10.1016/j.ijfoodmicro.2021.109089
PMID: 33662900
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3460
DOI:10.1016/j.ijfoodmicro.2021.109089