دورية أكاديمية
Appearances are deceptive: Three RNA viruses co-infected with the nucleopolyhedrovirus in host Lymantria dispar.
| العنوان: | Appearances are deceptive: Three RNA viruses co-infected with the nucleopolyhedrovirus in host Lymantria dispar. |
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| المؤلفون: | Pavlushin SV; Laboratory of Ecological Physiology, Institute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, Novosibirsk, 630091, Russia. Electronic address: sergey-pavlushin@mail.ru., Ilinsky YY; Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, street Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia. Electronic address: paulee@bionet.nsc.ru., Belousova IA; Laboratory of Ecological Physiology, Institute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, Novosibirsk, 630091, Russia. Electronic address: belousova_i@yahoo.com., Bayborodin SI; Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, street Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia. Electronic address: bai@bionet.nsc.ru., Lunev EA; Immanuel Kant Baltic Federal University, street A. Nevskogo 14, Kaliningrad, 236016, Russia. Electronic address: lunev357@gmail.com., Kechin AA; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, street Prospekt Lavrentyeva 8, Novosibirsk, 630090, Russia; Novosibirsk State University, street Pirogova 2, Novosibirsk, 630090, Russia. Electronic address: aa_kechin@niboch.nsc.ru., Khrapov EA; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, street Prospekt Lavrentyeva 8, Novosibirsk, 630090, Russia. Electronic address: khrap80@gmail.com., Filipenko ML; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, street Prospekt Lavrentyeva 8, Novosibirsk, 630090, Russia; Novosibirsk State University, street Pirogova 2, Novosibirsk, 630090, Russia. Electronic address: max@niboch.nsc.ru., Toshchakov SV; Winogradsky Institute of Microbiology, FRC Biotechnology, RAS, street Prospekt 60 let oktyabrya, 7/2, Moscow, 117312, Russia. Electronic address: stepan.toshchakov@gmail.com., Martemyanov VV; Laboratory of Ecological Physiology, Institute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, Novosibirsk, 630091, Russia; Reshetnev Siberian State University of Science and Technology, Krasnoiarskii rabochii av. 31, Krasnoyarsk, 630091, Russia. Electronic address: martemyanov79@yahoo.com. |
| المصدر: | Virus research [Virus Res] 2021 May; Vol. 297, pp. 198371. Date of Electronic Publication: 2021 Mar 05. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8410979 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7492 (Electronic) Linking ISSN: 01681702 NLM ISO Abbreviation: Virus Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier Science, c1984- |
| مواضيع طبية MeSH: | Moths* , Nucleopolyhedroviruses*/genetics , RNA Viruses*/genetics, Animals ; Larva |
| مستخلص: | The virus infection, which visually looks like typical monoinfection, in fact may hide a great complex of different species. Without detailed analysis, we may miss the important interaction between pathogens, including new species. In the current study, we found the new species inside the mix of cubic and polyhedral occlusion bodies (OBs) isolated from the gypsy moth, Lymantria dispar L. (Ld). Transmission electron microscopy (TEM) revealed that into the one cadaver were OBs which belonged to baculovirus and cypoviruses. The baculovirus produced polyhedral OBs, while cypoviruses produced polyhedral and cubic OBs. Genomic analysis detected the multiple Ld nucleopolyhedroviruses, and cypoviruses were Hubei lepidoptera virus 3 and Dendrolimus punctatus cypovirus 1. This represents the first isolation of the Hubei lepidoptera virus 3 from the gypsy moth, proposed as "Lymantria dispar cypovirus 3". The RNAseq analysis also revealed the presence of Lymantria dispar iflavirus 1. The insecticidal activity of the mixed infection was comparable to that of typical baculovirus monoinfection. Thus, we demonstrate that i) the shape of OBs identified by light microscopy cannot be a robust indicator of viral species infecting the host; ii) only specific analysis may reveal the true composition of viral infection. (Copyright © 2021 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Baculovirus; Genome sequence; Hubei lepidoptera virus 3; Iflaviridae; Mix infection; Reoviridae |
| تواريخ الأحداث: | Date Created: 20210308 Date Completed: 20220414 Latest Revision: 20220414 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.virusres.2021.198371 |
| PMID: | 33684420 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7492 |
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| DOI: | 10.1016/j.virusres.2021.198371 |