دورية أكاديمية
Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi.
العنوان: | Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi. |
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المؤلفون: | Beregovaya KA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia., Myshkina NM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia., Chepurnykh TV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia., Kotlobay AA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia., Purtov KV; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia. purtovk@mail.ru., Petushkov VN; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia., Rodionova NS; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia., Yampolsky IV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia. |
المصدر: | Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2021 May; Vol. 496 (1), pp. 14-17. Date of Electronic Publication: 2021 Mar 10. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Pleiades Publishing Country of Publication: United States NLM ID: 101126895 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1608-3091 (Electronic) Linking ISSN: 16076729 NLM ISO Abbreviation: Dokl Biochem Biophys Subsets: MEDLINE |
أسماء مطبوعة: | Publication: New York, NY : Pleiades Publishing Original Publication: Moscow, Russia : [Dordrecht] : International Academic Pub. Co. "Nauka/Interperiodica" ; Distributed worldwide by Kluwer Academic/Plenum Publishers, c2001- |
مواضيع طبية MeSH: | Agaricales/*enzymology , Fungal Proteins/*chemistry , Luciferases/*chemistry, Agaricales/genetics ; Amino Acid Sequence ; Catalytic Domain ; Computational Biology/methods ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Luciferases/genetics ; Luciferases/metabolism ; Luminescence ; Models, Molecular ; Mutagenesis ; Mutation ; Sequence Homology, Amino Acid ; Structure-Activity Relationship |
مستخلص: | The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination. |
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فهرسة مساهمة: | Keywords: Neonothopanus nambi; bioluminescence; luciferase; rational design |
المشرفين على المادة: | 0 (Fungal Proteins) EC 1.13.12.- (Luciferases) |
SCR Organism: | Neonothopanus nambi |
تواريخ الأحداث: | Date Created: 20210310 Date Completed: 20210722 Latest Revision: 20210722 |
رمز التحديث: | 20231215 |
DOI: | 10.1134/S1607672921010026 |
PMID: | 33689067 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1608-3091 |
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DOI: | 10.1134/S1607672921010026 |