دورية أكاديمية
أعرض في EDS

Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi.

التفاصيل البيبلوغرافية
العنوان: Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi.
المؤلفون: Beregovaya KA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia., Myshkina NM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia., Chepurnykh TV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia., Kotlobay AA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia., Purtov KV; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia. purtovk@mail.ru., Petushkov VN; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia., Rodionova NS; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia., Yampolsky IV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.
المصدر: Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2021 May; Vol. 496 (1), pp. 14-17. Date of Electronic Publication: 2021 Mar 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pleiades Publishing Country of Publication: United States NLM ID: 101126895 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1608-3091 (Electronic) Linking ISSN: 16076729 NLM ISO Abbreviation: Dokl Biochem Biophys Subsets: MEDLINE
أسماء مطبوعة: Publication: New York, NY : Pleiades Publishing
Original Publication: Moscow, Russia : [Dordrecht] : International Academic Pub. Co. "Nauka/Interperiodica" ; Distributed worldwide by Kluwer Academic/Plenum Publishers, c2001-
مواضيع طبية MeSH: Agaricales/*enzymology , Fungal Proteins/*chemistry , Luciferases/*chemistry, Agaricales/genetics ; Amino Acid Sequence ; Catalytic Domain ; Computational Biology/methods ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Luciferases/genetics ; Luciferases/metabolism ; Luminescence ; Models, Molecular ; Mutagenesis ; Mutation ; Sequence Homology, Amino Acid ; Structure-Activity Relationship
مستخلص: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination.
References: Yan, Y., Shi, P., Song, W., and Bi, S., Chemiluminescence and bioluminescence imaging for biosensing and therapy: in vitro and in vivo perspectives, Theranostics, 2019, vol. 9, pp. 4047–4065. https://doi.org/10.7150/thno.33228. (PMID: 10.7150/thno.33228312815316592176)
Fleiss, A. and Sarkisyan, K.S., A brief review of bioluminescent systems, Curr. Genet., 2019, vol. 65, pp. 877–882. https://doi.org/10.1007/s00294-019-00951-5. (PMID: 10.1007/s00294-019-00951-5308508676620254)
Pomper, M.G. and Gelovani, J.G., Molecular Imaging in Oncology, CRC Press, 2008. (PMID: 10.3109/9781420019957)
Chew, A.L.C., Desjardin, D.E., Tan, Y.-S., et al., Bioluminescent fungi from Peninsular Malaysia—a taxonomic and phylogenetic overview, Fungal Diversity, 2015, vol. 70, pp. 149–187. https://doi.org/10.1007/s13225-014-0302-9. (PMID: 10.1007/s13225-014-0302-9)
Shimomura, O., Bioluminescence: Chemical Principles and Methods, Singapore: World Scientific Publishing, 2006. https://doi.org/10.1142/6102. (PMID: 10.1142/6102)
Kotlobay, A.A., Sarkisyan, K.S., Mokrushina, Y.A., et al., Genetically encodable bioluminescent system from fungi, Proc. Natl. Acad. Sci. U. S. A., 2018, vol. 115, pp. 12728–12732. https://doi.org/10.1073/pnas.1803615115. (PMID: 10.1073/pnas.1803615115304780376294908)
Mitiouchkina, T., Mishin, A.S., Somermeyer, L.G., et al., Plants with genetically encoded autoluminescence, Nat. Biotechnol., 2020. https://doi.org/10.1038/s41587-020-0500-9.
Oliveira, A.G., Desjardin, D.E., Perry, B.A., and Stevani, C.V., Evidence that a single bioluminescent system is shared by all known bioluminescent fungal lineages, Photochem. Photobiol. Sci., 2012, vol. 11, pp. 848–852. https://doi.org/10.1039/c2pp25032b. (PMID: 10.1039/c2pp25032b22495263)
Kaskova, Z.M., Dörr, F.A., Petushkov, V.N., et al., Mechanism and color modulation of fungal bioluminescence, Sci. Adv., 2017, p. e1602847. https://doi.org/10.1126/sciadv.1602847.
Bok, J.W. and Keller, N.P., Fast and easy method for construction of plasmid vectors using modified quick-change mutagenesis, Methods Biochem. Analysis, 2012, vol. 944, pp. 163–174. https://doi.org/10.1007/978-1-62703-122-6_11. (PMID: 10.1007/978-1-62703-122-6_11)
Pichia expression kit. A manual of methods for expression of recombinant proteins using pPICZ and PICZ-alpha, in Pichia pastoris Catalog, 2009. http://tools.thermofisher.com/content/sfs/manuals/pich_man.pdf.
Kwolek-Mirek, M. and Zadrag-Tecza, R., Comparison of methods used for assessing the viability and vitality of yeast cells, FEMS Yeast Res., 2014, vol. 14, pp. 1068–1079. https://doi.org/10.1111/1567-1364.12202. (PMID: 10.1111/1567-1364.1220225154541)
فهرسة مساهمة: Keywords: Neonothopanus nambi; bioluminescence; luciferase; rational design
المشرفين على المادة: 0 (Fungal Proteins)
EC 1.13.12.- (Luciferases)
SCR Organism: Neonothopanus nambi
تواريخ الأحداث: Date Created: 20210310 Date Completed: 20210722 Latest Revision: 20210722
رمز التحديث: 20231215
DOI: 10.1134/S1607672921010026
PMID: 33689067
قاعدة البيانات: MEDLINE
الوصف
تدمد:1608-3091
DOI:10.1134/S1607672921010026