دورية أكاديمية
Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle.
| العنوان: | Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle. |
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| المؤلفون: | Singina GN; Ernst Federal Science Center for Animal Husbandry, Podolsk, Russia. g_singina@mail.ru., Sergiev PV; Institute of Functional Genomics, Moscow State University, Moscow, Russia.; Center of Life Sciences, Skolkovo Institute of Science and Technology, Skolkovo, Russia.; Faculty of Chemistry, Moscow State University, Moscow, Russia., Lopukhov AV; Ernst Federal Science Center for Animal Husbandry, Podolsk, Russia., Rubtsova MP; Faculty of Chemistry, Moscow State University, Moscow, Russia., Taradajnic NP; Ernst Federal Science Center for Animal Husbandry, Podolsk, Russia., Ravin NV; Research Center of Biotechnology, Moscow, Russia., Shedova EN; Ernst Federal Science Center for Animal Husbandry, Podolsk, Russia., Taradajnic TE; Ernst Federal Science Center for Animal Husbandry, Podolsk, Russia., Polejaeva IA; Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, USA., Dozev AV; Ernst Federal Science Center for Animal Husbandry, Podolsk, Russia., Brem G; Department of Animal Breeding and Genetics, University of Veterinary Medicine, Vienna, Austria., Dontsova OA; Center of Life Sciences, Skolkovo Institute of Science and Technology, Skolkovo, Russia.; Faculty of Chemistry, Moscow State University, Moscow, Russia.; Belozersky Research Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia., Zinovieva NA; Ernst Federal Science Center for Animal Husbandry, Podolsk, Russia. |
| المصدر: | Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2021 May; Vol. 496 (1), pp. 48-51. Date of Electronic Publication: 2021 Mar 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pleiades Publishing Country of Publication: United States NLM ID: 101126895 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1608-3091 (Electronic) Linking ISSN: 16076729 NLM ISO Abbreviation: Dokl Biochem Biophys Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Pleiades Publishing Original Publication: Moscow, Russia : [Dordrecht] : International Academic Pub. Co. "Nauka/Interperiodica" ; Distributed worldwide by Kluwer Academic/Plenum Publishers, c2001- |
| مواضيع طبية MeSH: | CRISPR-Cas Systems*, Animals, Genetically Modified/*genetics , Cattle/*genetics , Cloning, Organism/*methods , Embryo, Mammalian/*cytology , Fibroblasts/*cytology , Gene Editing/*methods, Animals ; Animals, Genetically Modified/embryology ; Cattle/embryology ; Embryo, Mammalian/metabolism ; Fibroblasts/metabolism ; Gene Knockout Techniques/methods ; Nuclear Transfer Techniques |
| مستخلص: | Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out genes for beta-lactoglobulin gene (PAEP) and the beta-lactoglobulin-like protein gene (LOC100848610) in the fibroblast cells. Gene editing (GE) efficiency was 4.4% for each of these genes. We successfully obtained single-cell-derived fibroblast colonies containing PAEP and LOC100848610 knockouts, which will be used to produce beta-lactoglobulin-deficient cattle. |
| References: | PLoS One. 2017 Oct 10;12(10):e0186056. (PMID: 29016691) J Biotechnol. 2006 Jul 13;124(2):469-72. (PMID: 16716426) Cell Res. 2011 Nov;21(11):1638-40. (PMID: 21912434) J Dairy Sci. 2008 Dec;91(12):4466-76. (PMID: 19038921) Nat Protoc. 2013 Nov;8(11):2281-2308. (PMID: 24157548) Science. 2013 Feb 15;339(6121):819-23. (PMID: 23287718) |
| فهرسة مساهمة: | Keywords: Bos taurus; beta-lactoglobulin knock-out; gene editing; somatic cloning |
| تواريخ الأحداث: | Date Created: 20210310 Date Completed: 20210722 Latest Revision: 20210722 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC7946654 |
| DOI: | 10.1134/S1607672921010099 |
| PMID: | 33689075 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1608-3091 |
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| DOI: | 10.1134/S1607672921010099 |