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Molecular Identification and Characterization of Plasmodium ovale curtisi in Field Isolates from Symptomatic Children in North-Central Nigeria.

التفاصيل البيبلوغرافية
العنوان: Molecular Identification and Characterization of Plasmodium ovale curtisi in Field Isolates from Symptomatic Children in North-Central Nigeria.
المؤلفون: Oyedeji SI; Molecular Parasitology and Genetics Unit, Department of Animal and Environmental Biology, Federal University Oye-Ekiti, Oye-Ekiti, Nigeria. segun.oyedeji@fuoye.edu.ng., Awobode HO; Parasitology Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria., Ojurongbe O; Department of Medical Microbiology and Parasitology, Ladoke Akintola University of Technology, Osogbo, Nigeria., Anumudu C; Parasitology Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria., Bassi PU; Department of Clinical Pharmacology and Therapeutics, University of Abuja, Abuja, Nigeria.
المصدر: Acta parasitologica [Acta Parasitol] 2021 Sep; Vol. 66 (3), pp. 915-924. Date of Electronic Publication: 2021 Mar 12.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 9301947 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1896-1851 (Electronic) Linking ISSN: 12302821 NLM ISO Abbreviation: Acta Parasitol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2019- : Cham : Springer International Publishing
Original Publication: Warszawa : Witold Stefanski Institute Of Parasitology
مواضيع طبية MeSH: Malaria*/epidemiology , Plasmodium ovale*/genetics, Child ; Humans ; Nigeria ; Sequence Analysis, DNA ; Species Specificity
مستخلص: Purpose: Plasmodium ovale is not usually the focus of most malaria research or intervention programmes and has lately been termed the neglected human malaria parasites. The parasite exists as two genetically distinct sympatric species namely P. ovale curtisi and P. ovale wallikeri but information on the distribution of P. ovale sub-species is lacking in Nigeria. The objective of this study, therefore, was aimed at characterizing the P. ovale sub-species in isolates from symptomatic individuals in North-central Nigeria.
Methods: Parasites were identified by light microscopy of Giemsa stained thick and thin blood films. Molecular characterization and confirmation of P. ovale sub-species were done by species-specific nested PCR and sequencing of the small subunit ribosomal RNA (SSUrRNA) gene.
Results: A total of 412 children were enrolled into this study of which 88.6% (n = 365) were positive for Plasmodium species by nested PCR and P. falciparum was predominant. Of the 365 isolates, 4 (1.1%) had P. ovale infections and of these, 3 (0.8%) were mixed species infections of P. ovale with P. falciparum. DNA sequence analysis confirmed that all the four P. ovale parasites were P. ovale curtisi as their sequences were 99-100% identical to previously published P. ovale curtisi sequences in the GenBank and they cluster with the P. ovale curtisi sequences by phylogeny.
Conclusion: Our findings demonstrate the occurrence of P. ovale curtisi in the study area. This has implications for public health and malaria elimination programmes, since they also serve as potential risk to travellers from malaria-free regions.
(© 2021. Witold Stefański Institute of Parasitology, Polish Academy of Sciences.)
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فهرسة مساهمة: Keywords: Malaria; Nigeria; PCR; Plasmodium ovale; SSUrRNA
تواريخ الأحداث: Date Created: 20210312 Date Completed: 20210831 Latest Revision: 20210831
رمز التحديث: 20221213
DOI: 10.1007/s11686-021-00350-2
PMID: 33710479
قاعدة البيانات: MEDLINE
الوصف
تدمد:1896-1851
DOI:10.1007/s11686-021-00350-2