The immunogenic maturation of goat monocyte-derived dendritic cells and upregulation of toll-like receptors by five antigens of Haemonchus contortus in-vitro.

التفاصيل البيبلوغرافية
العنوان:	The immunogenic maturation of goat monocyte-derived dendritic cells and upregulation of toll-like receptors by five antigens of Haemonchus contortus in-vitro.
المؤلفون:	Hasan MW; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: 2015207037@njau.edu.cn., Haseeb M; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: 2016207041@njau.edu.cn., Ehsan M; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China. Electronic address: mehsan124@gmail.com., Gadahi JA; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China; Department of Veterinary Parasitology, Sindh Agriculture University Tandojam, Pakistan., Wang Q; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: 2016107053@njau.edu.cn., Memon MA; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: 2016207040@njau.edu.cn., Aleem MT; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: 2018207076@njau.edu.cn., Lakho SA; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: 2017207046@njau.edu.cn., Yan RF; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: yanruofeng@njau.edu.cn., Xu LX; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: xulixin@njau.edu.cn., Song XK; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: songxiaokai@njau.edu.cn., Li X; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: lixiangrui@njau.edu.cn.
المصدر:	Research in veterinary science [Res Vet Sci] 2021 May; Vol. 136, pp. 247-258. Date of Electronic Publication: 2021 Mar 09.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: British Veterinary Association Country of Publication: England NLM ID: 0401300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2661 (Electronic) Linking ISSN: 00345288 NLM ISO Abbreviation: Res Vet Sci Subsets: MEDLINE
أسماء مطبوعة:	Publication: London : British Veterinary Association Original Publication: Oxford.
مواضيع طبية MeSH:	Up-Regulation, Antigens, Helminth/immunology , Dendritic Cells/immunology , Goats/immunology , Haemonchus/immunology , Toll-Like Receptors/genetics, Animals ; Monocytes/immunology ; Toll-Like Receptors/metabolism
مستخلص:	Previously, it was found that several proteins of Haemonchus contortus were involved in the stimulation of the host immune system. However, the information about the selection of superlative antigens with immunogenic efficacies on host DCs is lacking. In the current study, the stimulatory effects of five recombinant proteins (elongation factor-1α, arginine kinase, ES-15, ES-24, and ADP-ribosylation factor 1) of H. contortus on the maturation of goat monocyte-derived dendritic cells (md-DCs) were reported. Recombinant proteins were purified separately in E. coli expression and incubated with isolated goat peripheral blood mononuclear cells (PBMC). Immunofluorescence assay (IFA) results confirmed the binding of these molecules to the md-DC's surface as compared to control groups. In the flow cytometry analysis, recombinant proteins induced md-DC stimulation via the up-regulation of the expression of the costimulatory molecule (CD80) and MHC-II. Quantitative RT-PCR data showed a significant increase in the expression of specific genes of the WNT and toll-like receptor (TLR) signaling pathways. The result of ELISA indicated the higher levels of cytokine (IL-10, IL-12, IFN-γ, and TNF-α) secretion in the md-DC compared to the negative (pET-32a His-Tag) and blank (PBS) control groups. The data gives valuable support in the selection of potential antigens for future studies on the immunomodulation of the host against the infection of H. contortus. (Copyright © 2021 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة:	Keywords: Activation of the immune response; Dendritic cell functions; Goat monocytes; Haemonchus contortus, Immuno-stimulatory antigens
المشرفين على المادة:	0 (Antigens, Helminth) 0 (Toll-Like Receptors)
تواريخ الأحداث:	Date Created: 20210315 Date Completed: 20210528 Latest Revision: 20210528
رمز التحديث:	20221213
DOI:	10.1016/j.rvsc.2021.03.007
PMID:	33721712
قاعدة البيانات:	MEDLINE

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تدمد:	1532-2661
DOI:	10.1016/j.rvsc.2021.03.007