دورية أكاديمية
أعرض في EDS

Functional characterization of two 20β-hydroxysteroid dehydrogenase type 2 homeologs from Xenopus laevis reveals multispecificity.

التفاصيل البيبلوغرافية
العنوان: Functional characterization of two 20β-hydroxysteroid dehydrogenase type 2 homeologs from Xenopus laevis reveals multispecificity.
المؤلفون: Tokarz J; Helmholtz Zentrum München, German Research Center for Environmental Health, Research Unit Molecular Endocrinology and Metabolism, Neuherberg, Germany. Electronic address: janina.tokarz@helmholtz-muenchen.de., Schmitt SM; Walter Brendel Centre of Experimental Medicine, University Hospital and Ludwig-Maximilians-University Munich, Munich, Germany., Möller G; Helmholtz Zentrum München, German Research Center for Environmental Health, Research Unit Molecular Endocrinology and Metabolism, Neuherberg, Germany., Brändli AW; Walter Brendel Centre of Experimental Medicine, University Hospital and Ludwig-Maximilians-University Munich, Munich, Germany., Adamski J; Helmholtz Zentrum München, German Research Center for Environmental Health, Research Unit Molecular Endocrinology and Metabolism, Neuherberg, Germany; German Center for Diabetes Research, Neuherberg, Germany; Lehrstuhl für Experimentelle Genetik, Technische Universität München, Freising-Weihenstephan, Germany; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
المصدر: The Journal of steroid biochemistry and molecular biology [J Steroid Biochem Mol Biol] 2021 Jun; Vol. 210, pp. 105874. Date of Electronic Publication: 2021 Mar 17.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Pergamon Country of Publication: England NLM ID: 9015483 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1220 (Electronic) Linking ISSN: 09600760 NLM ISO Abbreviation: J Steroid Biochem Mol Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford ; New York : Pergamon, c1990-
مواضيع طبية MeSH: Cortisone Reductase/*genetics , Cortisone Reductase/*metabolism , Xenopus Proteins/*genetics , Xenopus Proteins/*metabolism , Xenopus laevis/*genetics, 17-alpha-Hydroxyprogesterone/metabolism ; Animals ; Cortisone/metabolism ; Embryo, Nonmammalian ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Enzymologic ; HeLa Cells ; Humans ; Phylogeny ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Substrate Specificity ; Xenopus laevis/embryology
مستخلص: The African clawed frog, Xenopus laevis, is a versatile model for biomedical research and is largely similar to mammals in terms of organ development, anatomy, physiology, and hormonal signaling mechanisms. Steroid hormones control a variety of processes and their levels are regulated by hydroxysteroid dehydrogenases (HSDs). The subfamily of 20β-HSD type 2 enzymes currently comprises eight members from teleost fish and mammals. Here, we report the identification of three 20β-HSD type 2 genes in X. tropicalis and X. laevis and the functional characterization of the two homeologs from X. laevis. X. laevis Hsd20b2.L and Hsd20b2.S showed high sequence identity with known 20β-HSD type 2 enzymes and mapped to the two subgenomes of the allotetraploid frog genome. Both homeologs are expressed during embryonic development and in adult tissues, with strongest signals in liver, kidney, intestine, and skin. After recombinant expression in human cell lines, both enzymes co-localized with the endoplasmic reticulum and catalyzed the conversion of cortisone to 20β-dihydrocortisone. Both Hsd20b2.L and Hsd20b2.S catalyzed the 20β-reduction of further C 21 steroids (17α-hydroxyprogesterone, progesterone, 11-deoxycortisol, 11-deoxycorticosterone), while only Hsd20b2.S was able to convert corticosterone and cortisol to their 20β-reduced metabolites. Estrone was only a poor and androstenedione no substrate for both enzymes. Our results demonstrate multispecificity of 20β-HSD type 2 enzymes from X. laevis similar to other teleost 20β-HSD type 2 enzymes. X. laevis 20β-HSD type 2 enzymes are probably involved in steroid catabolism and in the generation of pheromones for intraspecies communication. A role in oocyte maturation is unlikely.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: 20β-reduction; Cortisone; Frog; Hydroxysteroid dehydrogenase; Steroid catabolism; hsd20b2
المشرفين على المادة: 0 (Recombinant Proteins)
0 (Xenopus Proteins)
68-96-2 (17-alpha-Hydroxyprogesterone)
EC 1.1.1.53 (Cortisone Reductase)
V27W9254FZ (Cortisone)
تواريخ الأحداث: Date Created: 20210316 Date Completed: 20210827 Latest Revision: 20210827
رمز التحديث: 20221213
DOI: 10.1016/j.jsbmb.2021.105874
PMID: 33722706
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-1220
DOI:10.1016/j.jsbmb.2021.105874