تقرير
Fasting-induced FOXO4 blunts human CD4 + T helper cell responsiveness.
| العنوان: | Fasting-induced FOXO4 blunts human CD4 + T helper cell responsiveness. |
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| المؤلفون: | Han K; Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Singh K; Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.; Bioinformatics and Computational Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Rodman MJ; Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Hassanzadeh S; Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Wu K; Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Nguyen A; Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Huffstutler RD; Cardiovascular Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Seifuddin F; Bioinformatics and Computational Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Dagur PK; Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Saxena A; Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., McCoy JP; Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Chen J; Center of Human Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., Biancotto A; Center of Human Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.; Department of Precision Immunology, Sanofi, Cambridge, MA, USA., Stagliano KER; Center of Human Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., Teague HL; Laboratory of Cardiometabolic Disease and Inflammation, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Mehta NN; Cardiovascular Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.; Laboratory of Cardiometabolic Disease and Inflammation, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Pirooznia M; Bioinformatics and Computational Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA., Sack MN; Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. sackm@nih.gov.; Cardiovascular Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. sackm@nih.gov. |
| المصدر: | Nature metabolism [Nat Metab] 2021 Mar; Vol. 3 (3), pp. 318-326. Date of Electronic Publication: 2021 Mar 15. |
| نوع المنشور: | Letter; Research Support, N.I.H., Intramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Nature Country of Publication: Germany NLM ID: 101736592 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2522-5812 (Electronic) Linking ISSN: 25225812 NLM ISO Abbreviation: Nat Metab Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin : Springer Nature, [2019]- |
| مواضيع طبية MeSH: | Fasting*, Cell Cycle Proteins/*biosynthesis , Forkhead Transcription Factors/*biosynthesis , T-Lymphocytes, Helper-Inducer/*immunology, Gene Expression Regulation ; Humans ; Sequence Analysis, RNA |
| مستخلص: | Intermittent fasting blunts inflammation in asthma 1 and rheumatoid arthritis 2 , suggesting that fasting may be exploited as an immune-modulatory intervention. However, the mechanisms underpinning the anti-inflammatory effects of fasting are poorly characterized 3-5 . Here, we show that fasting in humans is sufficient to blunt CD4 + T helper cell responsiveness. RNA sequencing and flow cytometry immunophenotyping of peripheral blood mononuclear cells from volunteers subjected to overnight or 24-h fasting and 3 h of refeeding suggest that fasting blunts CD4 + T helper cell activation and differentiation. Transcriptomic analysis reveals that longer fasting has a more robust effect on CD4 + T-cell biology. Through bioinformatics analyses, we identify the transcription factor FOXO4 and its canonical target FK506-binding protein 5 (FKBP5) as a potential fasting-responsive regulatory axis. Genetic gain- or loss-of-function of FOXO4 and FKBP5 is sufficient to modulate T |
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| معلومات مُعتمدة: | ZIA HL005102 United States ImNIH Intramural NIH HHS; ZIA HL005199 United States ImNIH Intramural NIH HHS; ZIC HL006199 United States ImNIH Intramural NIH HHS |
| سلسلة جزيئية: | ClinicalTrials.gov NCT02719899; NCT01143454; NCT00001846 |
| المشرفين على المادة: | 0 (Cell Cycle Proteins) 0 (FOXO4 protein, human) 0 (Forkhead Transcription Factors) |
| تواريخ الأحداث: | Date Created: 20210316 Date Completed: 20210423 Latest Revision: 20221027 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC7990708 |
| DOI: | 10.1038/s42255-021-00356-0 |
| PMID: | 33723462 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. | تدمد: | 2522-5812 |
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| DOI: | 10.1038/s42255-021-00356-0 |