دورية أكاديمية
Homogeneously Staining Regions (HSR) in Chromosome 1 of the House Mouse: Synapsis and Recombination at Meiosis.
| العنوان: | Homogeneously Staining Regions (HSR) in Chromosome 1 of the House Mouse: Synapsis and Recombination at Meiosis. |
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| المؤلفون: | Torgunakov NY; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation.; Novosibirsk State University, Novosibirsk, Russian Federation., Kizilova EA; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation.; Novosibirsk State University, Novosibirsk, Russian Federation., Karamysheva TV; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation., Malinovskaya LP; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation.; Novosibirsk State University, Novosibirsk, Russian Federation., Bikchurina TI; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation.; Novosibirsk State University, Novosibirsk, Russian Federation., Borodin PM; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation, borodin@bionet.nsc.ru.; Novosibirsk State University, Novosibirsk, Russian Federation, borodin@bionet.nsc.ru. |
| المصدر: | Cytogenetic and genome research [Cytogenet Genome Res] 2021; Vol. 161 (1-2), pp. 14-22. Date of Electronic Publication: 2021 Mar 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: S. Karger Country of Publication: Switzerland NLM ID: 101142708 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1424-859X (Electronic) Linking ISSN: 14248581 NLM ISO Abbreviation: Cytogenet Genome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel ; New York : S. Karger, c2002- |
| مواضيع طبية MeSH: | Chromosome Mapping* , Meiosis* , Recombination, Genetic*, Animals ; Cell Nucleus/metabolism ; Chromosome Aberrations ; Chromosome Banding ; DNA/genetics ; Epigenesis, Genetic ; Female ; Heterozygote ; Histones/genetics ; Homozygote ; In Situ Hybridization, Fluorescence ; Karyotyping ; Male ; Mice ; Mice, Inbred C57BL ; Multigene Family ; Spermatocytes/cytology |
| مستخلص: | Amplified sequences constitute a large part of mammalian genomes. A chromosome 1 containing 2 large (up to 50 Mb) homogeneously staining regions (HSRs) separated by a small inverted euchromatic region is present in many natural populations of the house mouse (Mus musculus musculus). The HSRs are composed of a long-range repeat cluster, Sp100-rs, with a repeat length of 100 kb. In order to understand the organization and function of HSRs in meiotic chromosomes, we examined synapsis and recombination in male mice hetero- and homozygous for the HSR-carrying chromosome using FISH with an HSR-specific DNA probe and immunolocalization of the key meiotic proteins. In all homozygous and heterozygous pachytene nuclei, we observed fully synapsed linear homomorphic bivalents 1 marked by the HSR FISH probe. The synaptic adjustment in the heterozygotes was bilateral: the HSR-carrying homolog was shortened and the wild-type homolog was elongated. The adjustment was reversible: desynapsis at diplotene was accompanied by elongation of the HSRs. Immunolocalization of H3K9me2/3 indicated that the HSRs in the meiotic chromosome retained the epigenetic modification typical for C-heterochromatin in somatic cells. MLH1 foci, marking mature recombination nodules, were detected in the proximal HSR band in heterozygotes and in both HSR bands of homozygotes. Unequal crossing over within the long-range repeat cluster can cause variation in size of the HSRs, which has been detected in the natural populations of the house mouse. (© 2021 S. Karger AG, Basel.) |
| فهرسة مساهمة: | Keywords: Insertion; Inversion; MLH1; Synaptic adjustment; Synaptonemal complex |
| المشرفين على المادة: | 0 (Histones) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20210316 Date Completed: 20210601 Latest Revision: 20210601 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1159/000513266 |
| PMID: | 33725692 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1424-859X |
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| DOI: | 10.1159/000513266 |