دورية أكاديمية
Functional domain studies uncover novel roles for the ZTL Kelch repeat domain in clock function.
العنوان: | Functional domain studies uncover novel roles for the ZTL Kelch repeat domain in clock function. |
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المؤلفون: | Feke A; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, United States of America., Vanderwall M; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, United States of America., Liu W; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, United States of America., Gendron JM; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, United States of America. |
المصدر: | PloS one [PLoS One] 2021 Mar 17; Vol. 16 (3), pp. e0235938. Date of Electronic Publication: 2021 Mar 17 (Print Publication: 2021). |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science |
مواضيع طبية MeSH: | Arabidopsis/*metabolism , Arabidopsis Proteins/*metabolism , Circadian Clocks/*physiology , Kelch Repeat/*genetics, Arabidopsis/growth & development ; Arabidopsis Proteins/chemistry ; Arabidopsis Proteins/genetics ; Chromatography, High Pressure Liquid ; Flowers/growth & development ; Mass Spectrometry ; Peptides/analysis ; Phenotype ; Plants, Genetically Modified/metabolism |
مستخلص: | The small LOV/F-box/Kelch family of E3 ubiquitin ligases plays an essential role in the regulation of plant circadian clocks and flowering time by sensing dusk. The family consists of three members, ZEITLUPE (ZTL), LOV KELCH PROTEIN 2 (LKP2), and FLAVIN-BINDING KELCH REPEAT F-BOX PROTEIN 1 (FKF1), which share a unique protein domain architecture allowing them to act as photoreceptors that transduce light signals via altering stability of target proteins. Despite intensive study of this protein family we still lack important knowledge about the biochemical and functional roles of the protein domains that comprise these unique photoreceptors. Here, we perform comparative analyses of transgenic lines constitutively expressing the photoreceptor LOV domain or the Kelch repeat protein-protein interaction domains of ZTL, FKF1, and LKP2. Expression of each domain alone is sufficient to disrupt circadian rhythms and flowering time, but each domain differs in the magnitude of effect. Immunoprecipitation followed by mass spectrometry with the ZTL Kelch repeat domain identified a suite of potential interacting partners. Furthermore, the ZTL Kelch repeat domain can interact with the ZTL homologs, LKP2 and FKF1, and the LOV domain of ZTL itself. This suggests a hypothesis that the Kelch repeat domain of ZTL may mediate inter- and intra-molecular interactions of the three LOV/F-box/Kelch proteins and provides added insight into the composition of the protein complexes and an additional role for the Kelch repeat domain. Competing Interests: The authors have declared that no competing interests exist. |
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معلومات مُعتمدة: | R35 GM128670 United States GM NIGMS NIH HHS; T32 GM007499 United States GM NIGMS NIH HHS |
المشرفين على المادة: | 0 (Arabidopsis Proteins) 0 (FKF1 protein, Arabidopsis) 0 (LKP2 protein, Arabidopsis) 0 (Peptides) 0 (ZTL protein, Arabidopsis) |
تواريخ الأحداث: | Date Created: 20210317 Date Completed: 20210831 Latest Revision: 20210831 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC7968664 |
DOI: | 10.1371/journal.pone.0235938 |
PMID: | 33730063 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1932-6203 |
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DOI: | 10.1371/journal.pone.0235938 |