دورية أكاديمية
أعرض في EDS

Functional domain studies uncover novel roles for the ZTL Kelch repeat domain in clock function.

التفاصيل البيبلوغرافية
العنوان: Functional domain studies uncover novel roles for the ZTL Kelch repeat domain in clock function.
المؤلفون: Feke A; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, United States of America., Vanderwall M; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, United States of America., Liu W; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, United States of America., Gendron JM; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, United States of America.
المصدر: PloS one [PLoS One] 2021 Mar 17; Vol. 16 (3), pp. e0235938. Date of Electronic Publication: 2021 Mar 17 (Print Publication: 2021).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Arabidopsis/*metabolism , Arabidopsis Proteins/*metabolism , Circadian Clocks/*physiology , Kelch Repeat/*genetics, Arabidopsis/growth & development ; Arabidopsis Proteins/chemistry ; Arabidopsis Proteins/genetics ; Chromatography, High Pressure Liquid ; Flowers/growth & development ; Mass Spectrometry ; Peptides/analysis ; Phenotype ; Plants, Genetically Modified/metabolism
مستخلص: The small LOV/F-box/Kelch family of E3 ubiquitin ligases plays an essential role in the regulation of plant circadian clocks and flowering time by sensing dusk. The family consists of three members, ZEITLUPE (ZTL), LOV KELCH PROTEIN 2 (LKP2), and FLAVIN-BINDING KELCH REPEAT F-BOX PROTEIN 1 (FKF1), which share a unique protein domain architecture allowing them to act as photoreceptors that transduce light signals via altering stability of target proteins. Despite intensive study of this protein family we still lack important knowledge about the biochemical and functional roles of the protein domains that comprise these unique photoreceptors. Here, we perform comparative analyses of transgenic lines constitutively expressing the photoreceptor LOV domain or the Kelch repeat protein-protein interaction domains of ZTL, FKF1, and LKP2. Expression of each domain alone is sufficient to disrupt circadian rhythms and flowering time, but each domain differs in the magnitude of effect. Immunoprecipitation followed by mass spectrometry with the ZTL Kelch repeat domain identified a suite of potential interacting partners. Furthermore, the ZTL Kelch repeat domain can interact with the ZTL homologs, LKP2 and FKF1, and the LOV domain of ZTL itself. This suggests a hypothesis that the Kelch repeat domain of ZTL may mediate inter- and intra-molecular interactions of the three LOV/F-box/Kelch proteins and provides added insight into the composition of the protein complexes and an additional role for the Kelch repeat domain.
Competing Interests: The authors have declared that no competing interests exist.
References: J Exp Bot. 2004 Sep;55(405):2015-27. (PMID: 15310821)
Mol Plant. 2012 May;5(3):573-82. (PMID: 22402262)
FEBS Lett. 2005 Feb 14;579(5):1067-71. (PMID: 15710392)
Plant Physiol. 2017 Jan;173(1):5-15. (PMID: 27688622)
Science. 2005 Jul 8;309(5732):293-7. (PMID: 16002617)
Proc Natl Acad Sci U S A. 2009 Jan 20;106(3):835-40. (PMID: 19126682)
Nature. 2003 Dec 4;426(6966):567-70. (PMID: 14654842)
Dev Cell. 2009 Jul;17(1):75-86. (PMID: 19619493)
Proc Natl Acad Sci U S A. 2010 May 18;107(20):9458-63. (PMID: 20439704)
Science. 2007 Oct 12;318(5848):261-5. (PMID: 17872410)
Elife. 2017 Feb 28;6:. (PMID: 28244872)
Biochemistry. 2013 Oct 8;52(40):7150-8. (PMID: 24033190)
Mol Cells. 2019 Oct 31;42(10):693-701. (PMID: 31617339)
Plant J. 1998 Dec;16(6):735-43. (PMID: 10069079)
Electrophoresis. 1999 Dec;20(18):3551-67. (PMID: 10612281)
Plant Cell Environ. 2017 Nov;40(11):2608-2627. (PMID: 28628949)
Plant J. 2004 Oct;40(2):291-301. (PMID: 15447654)
Methods Mol Biol. 2014;1158:13-44. (PMID: 24792042)
Plant Physiol. 2007 Jan;143(1):473-86. (PMID: 17098855)
Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11519-24. (PMID: 12169662)
Biophys J. 2010 Dec 1;99(11):3831-9. (PMID: 21112308)
J Proteomics. 2014 Apr 4;100:37-43. (PMID: 24513533)
Plant Physiol. 2018 Jul;177(3):1170-1186. (PMID: 29794020)
Science. 2009 Mar 13;323(5920):1481-5. (PMID: 19286557)
Plant J. 2011 Aug;67(4):608-21. (PMID: 21518052)
J Biol Methods. 2014;1(2):. (PMID: 25606571)
Plant J. 2010 Jan;61(2):259-70. (PMID: 19843313)
Cell. 2000 Apr 28;101(3):331-40. (PMID: 10847687)
Data Brief. 2016 May 14;8:56-60. (PMID: 27274533)
Nucleic Acids Res. 2019 Jan 8;47(D1):D506-D515. (PMID: 30395287)
Nat Methods. 2013 Aug;10(8):730-6. (PMID: 23921808)
Nat Commun. 2017 Dec;8(1):3. (PMID: 28232745)
J Biol Chem. 2008 Aug 22;283(34):23073-83. (PMID: 18562312)
Plant Cell. 2007 Aug;19(8):2516-30. (PMID: 17693530)
Enzymes. 2014;35:213-39. (PMID: 25740721)
Cell. 1996 Jul 26;86(2):263-74. (PMID: 8706131)
Mol Cell Proteomics. 2016 Jan;15(1):201-17. (PMID: 26545401)
Cell. 2000 Apr 28;101(3):319-29. (PMID: 10847686)
Nat Commun. 2019 Aug 21;10(1):3750. (PMID: 31434902)
Genetics. 2020 Jul;215(3):699-712. (PMID: 32434795)
Plant Cell. 2010 Mar;22(3):606-22. (PMID: 20354196)
Proc Natl Acad Sci U S A. 2011 Oct 4;108(40):16843-8. (PMID: 21949396)
Plant J. 2004 Sep;39(6):847-62. (PMID: 15341628)
Plant Cell. 2004 Mar;16(3):769-82. (PMID: 14973171)
J Exp Bot. 2019 May 9;70(10):2741-2757. (PMID: 30825377)
Plant Cell Physiol. 2005 Aug;46(8):1340-9. (PMID: 15937324)
Nat Rev Mol Cell Biol. 2009 Jun;10(6):385-97. (PMID: 19424292)
Science. 2012 May 25;336(6084):1045-9. (PMID: 22628657)
Nature. 2007 Sep 20;449(7160):356-60. (PMID: 17704763)
Nature. 2003 Nov 20;426(6964):302-6. (PMID: 14628054)
Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834)
Elife. 2019 Apr 05;8:. (PMID: 30950791)
Plant J. 2000 Sep;23(6):807-15. (PMID: 10998191)
Plant Cell. 2001 Dec;13(12):2659-70. (PMID: 11752379)
Plant Physiol. 2006 Mar;140(3):933-45. (PMID: 16428597)
Development. 2013 Oct;140(19):4060-9. (PMID: 24004949)
معلومات مُعتمدة: R35 GM128670 United States GM NIGMS NIH HHS; T32 GM007499 United States GM NIGMS NIH HHS
المشرفين على المادة: 0 (Arabidopsis Proteins)
0 (FKF1 protein, Arabidopsis)
0 (LKP2 protein, Arabidopsis)
0 (Peptides)
0 (ZTL protein, Arabidopsis)
تواريخ الأحداث: Date Created: 20210317 Date Completed: 20210831 Latest Revision: 20210831
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7968664
DOI: 10.1371/journal.pone.0235938
PMID: 33730063
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0235938