One-shot identification of SARS-CoV-2 S RBD escape mutants using yeast screening.
| العنوان: | One-shot identification of SARS-CoV-2 S RBD escape mutants using yeast screening. |
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| المؤلفون: | Urdaniz IF; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Steiner PJ; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Kirby MB; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Zhao F; The Scripps Research Institute, La Jolla, CA, USA., Haas CM; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Barman S; The Scripps Research Institute, La Jolla, CA, USA., Rhodes ER; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Peng L; The Scripps Research Institute, La Jolla, CA, USA., Sprenger KG; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Jardine JG; International AIDS Vaccine Initiative, New York, NY, USA., Whitehead TA; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2021 Mar 15. Date of Electronic Publication: 2021 Mar 15. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | The potential emergence of SARS-CoV-2 Spike (S) escape mutants is a threat to reduce the efficacy of existing vaccines and neutralizing antibody (nAb) therapies. An understanding of the antibody/S escape mutations landscape is urgently needed to preemptively address this threat. Here we describe a rapid method to identify escape mutants for nAbs targeting the S receptor binding site. We identified escape mutants for five nAbs, including three from the public germline class VH3-53 elicited by natural COVID-19 infection. Escape mutations predominantly mapped to the periphery of the ACE2 recognition site on the RBD with K417, D420, Y421, F486, and Q493 as notable hotspots. We provide libraries, methods, and software as an openly available community resource to accelerate new therapeutic strategies against SARS-CoV-2. Competing Interests: Competing Interests: IFU, PJS, MBK, CH, JJ, TAW declare competing interests. |
| التعليقات: | Update in: Cell Rep. 2021 Aug 31;36(9):109627. (PMID: 34416153) |
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| معلومات مُعتمدة: | R01 AI141452 United States AI NIAID NIH HHS |
| تواريخ الأحداث: | Date Created: 20210324 Latest Revision: 20240403 |
| رمز التحديث: | 20240403 |
| مُعرف محوري في PubMed: | PMC7987007 |
| DOI: | 10.1101/2021.03.15.435309 |
| PMID: | 33758848 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2021.03.15.435309 |
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