التفاصيل البيبلوغرافية
| العنوان: |
Tucumã ( Astrocaryum aculeatum ) Prevents Oxidative and DNA Damage to Retinal Pigment Epithelium Cells. |
| المؤلفون: |
Bonadiman BDSR; Department of Biochemistry, Biological Sciences Center, Federal University of Santa Catarina, Florianópolis, Brazil., Chaves C; Department of Ophthalmology, Health Science Center, Nilton Lins University, Manaus, Amazonas, Brazil., Assmann CE; Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria, Brazil., Weis GCC; Department of Food Science and Technology, Federal University of Santa Maria, Santa Maria, Brazil., Alves AO; Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, Brazil., Gindri AL; Department of Biological Sciences Center, Integrated Regional University of Upper Uruguay and the Missions (URI), Santiago, Brazil., Chaves C; Department of Ophthalmology, Ophthalmology Institute of Manaus, Manaus, Amazonas, Brazil., da Cruz IBM; Department of Morphology, Federal University of Santa Maria, Santa Maria, Brazil., Zamoner A; Department of Biochemistry, Biological Sciences Center, Federal University of Santa Catarina, Florianópolis, Brazil., Bagatini MD; Graduate Program in Biomedical Sciences, Campus Chapecó, Federal University of Fronteira Sul, Chapecó, Brazil. |
| المصدر: |
Journal of medicinal food [J Med Food] 2021 Oct; Vol. 24 (10), pp. 1050-1057. Date of Electronic Publication: 2021 Mar 25. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 9812512 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7600 (Electronic) Linking ISSN: 1096620X NLM ISO Abbreviation: J Med Food Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c1998- |
| مواضيع طبية MeSH: |
Arecaceae* , Retinal Pigment Epithelium*, DNA Damage ; Humans ; Oxidation-Reduction ; Oxidative Stress |
| مستخلص: |
Eye diseases have a negative impact on the eyesight quality of the world population. The age-related macular degeneration (AMD) draws special attention since it is a chronic disorder characterized by oxidative and inflammatory damage to the retinal epithelial pigment, which triggers progressive vision loss. In the Brazilian Amazon, Astrocaryum aculeatum is an Amazonian fruit ( Tucumã ) used by riverside communities in traditional medicine to treat a number of ailments . These communities have recently shown to have increased longevity and reduced prevalence of age-related morbidity. Thus, the aim of this research was to chemically characterize and analyze the in vitro antioxidant effect and molecular damage prevention of the Tucumã ethanolic extract in retinal pigment epithelium (RPE) cells in a model for AMD. The extract was chemically characterized by ultra-high-performance liquid chromatography (HPLC) coupled with diode-array detection and mass spectrophotometry (HPLC-DAD-MS). In vitro protocols were performed, and the cytopreventive effect of Tucumã on RPE cells exposed to high concentrations of superoxide anion, an oxidant and genotoxic molecule, as well as the effect of Tucumã extract on oxidative and molecular makers were assessed. Biochemical and flow cytometry analyses were conducted in these protocols. The extract presents high concentrations of caffeic acid, gallic acid, catechin, luteolin, quercetin, and rutin. Treatment did not show cytotoxic effects in cells treated only with extract at 50 μ g/mL. In fact, it improved cell viability and was able to prevent necrosis and apoptosis, and oxidative and molecular damage was significantly reduced. In summary, Tucumã is an important Amazon fruit, which seems to contribute significantly to improve human health conditions, as our findings suggest that its extract has a relevant chemical matrix rich in antioxidant molecules, and its consumption could improve eye health and contribute to prevention against oxidative stress through cytoprevention, reactive oxygen species reduction, and maintenance of DNA integrity in retinal pigment epithelium (RPE) cells. |
| فهرسة مساهمة: |
Keywords: Amazon fruit; DNA damage; antioxidant; elderly; eye |
| تواريخ الأحداث: |
Date Created: 20210326 Date Completed: 20211022 Latest Revision: 20211022 |
| رمز التحديث: |
20221213 |
| DOI: |
10.1089/jmf.2020.0171 |
| PMID: |
33769097 |
| قاعدة البيانات: |
MEDLINE |
