دورية أكاديمية
Transcribed germline-limited coding sequences in Oxytricha trifallax.
| العنوان: | Transcribed germline-limited coding sequences in Oxytricha trifallax. |
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| المؤلفون: | Miller RV; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA., Neme R; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.; Departamento de Quimica y Biologia, Universidad del Norte, Barranquilla, Colombia., Clay DM; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA., Pathmanathan JS; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA., Lu MW; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.; Department of Biological Sciences, Columbia University, New York, NY 10027, USA., Yerlici VT; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA., Khurana JS; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA., Landweber LF; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.; Department of Biological Sciences, Columbia University, New York, NY 10027, USA. |
| المصدر: | G3 (Bethesda, Md.) [G3 (Bethesda)] 2021 Jun 17; Vol. 11 (6). |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101566598 Publication Model: Print Cited Medium: Internet ISSN: 2160-1836 (Electronic) Linking ISSN: 21601836 NLM ISO Abbreviation: G3 (Bethesda) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [Oxford] : Oxford University Press Original Publication: Bethesda, MD : Genetics Society of America, 2011- |
| مواضيع طبية MeSH: | Oxytricha*/genetics, Animals ; Gene Rearrangement ; Germ Cells ; DNA/metabolism ; RNA/metabolism |
| مستخلص: | The germline-soma divide is a fundamental distinction in developmental biology, and different genes are expressed in germline and somatic cells throughout metazoan life cycles. Ciliates, a group of microbial eukaryotes, exhibit germline-somatic nuclear dimorphism within a single cell with two different genomes. The ciliate Oxytricha trifallax undergoes massive RNA-guided DNA elimination and genome rearrangement to produce a new somatic macronucleus (MAC) from a copy of the germline micronucleus (MIC). This process eliminates noncoding DNA sequences that interrupt genes and also deletes hundreds of germline-limited open reading frames (ORFs) that are transcribed during genome rearrangement. Here, we update the set of transcribed germline-limited ORFs (TGLOs) in O. trifallax. We show that TGLOs tend to be expressed during nuclear development and then are absent from the somatic MAC. We also demonstrate that exposure to synthetic RNA can reprogram TGLO retention in the somatic MAC and that TGLO retention leads to transcription outside the normal developmental program. These data suggest that TGLOs represent a group of developmentally regulated protein-coding sequences whose gene expression is terminated by DNA elimination. (© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.) |
| معلومات مُعتمدة: | R35 GM122555 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: DNA elimination; ciliate; genome rearrangement; germline; micronucleus; noncoding RNA |
| المشرفين على المادة: | 9007-49-2 (DNA) 63231-63-0 (RNA) |
| تواريخ الأحداث: | Date Created: 20210327 Date Completed: 20221111 Latest Revision: 20221117 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8495736 |
| DOI: | 10.1093/g3journal/jkab092 |
| PMID: | 33772542 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2160-1836 |
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| DOI: | 10.1093/g3journal/jkab092 |