دورية أكاديمية
Urinary microbiota; Which non-ınvasive urine collection method should we use?
| العنوان: | Urinary microbiota; Which non-ınvasive urine collection method should we use? |
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| المؤلفون: | Ozer MS; Department of Urology, School of Medicine, Dokuz Eylul University, Izmir, Turkey., Yildiz HA; Department of Urology, Mus Malazgirt State Hospital, Mus, Turkey., Incir C; Department of Medical Pharmacology, School of Medicine, Dokuz Eylul University, Izmir, Turkey., Deger MD; Department of Urology, Edirne Sultan 1. Murat State Hospital, Edirne, Turkey., Bozkurt O; Department of Urology, School of Medicine, Dokuz Eylul University, Izmir, Turkey., Ergor G; Department of Public Health, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey., Tuncok Y; Department of Medical Pharmacology, School of Medicine, Dokuz Eylul University, Izmir, Turkey., Esen N; Department of Microbiology, School of Medicine, Dokuz Eylul University, Izmir, Turkey., Esen AA; Department of Urology, School of Medicine, Dokuz Eylul University, Izmir, Turkey. |
| المصدر: | International journal of clinical practice [Int J Clin Pract] 2021 Jul; Vol. 75 (7), pp. e14193. Date of Electronic Publication: 2021 Apr 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Hindawi Country of Publication: India NLM ID: 9712381 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1742-1241 (Electronic) Linking ISSN: 13685031 NLM ISO Abbreviation: Int J Clin Pract Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : Mumbai : Hindawi Original Publication: Esher [England] ; Bronxville, N.Y. : Medicom International, c1997- |
| مواضيع طبية MeSH: | Microbiota* , Urinary Bladder Neoplasms*, Bacteria ; Humans ; Male ; RNA, Ribosomal, 16S/genetics ; Urine Specimen Collection |
| مستخلص: | Objective: The aim of this study is to establish the optimal non-invasive urine sample collection method for the microbiota studies. Methodology: Twelve men with bladder carcinoma underwent first voided and midstream urine collection. Urine samples were analysed using V3-V4 regions of bacterial 16s ribosomal RNAs. Bacterial groups with relative abundance above 1% were analysed in first voided urine and midstream urine samples at phylum, class, order and family level. At the genus level, all of the identified bacterial groups' relative abundances were analysed. The statistical significance (P < .05) of differences between first voided and midstream urine sample microbiota was evaluated using the Wilcoxon test. Results: According to the analysis, 8 phyla, 14 class, 23 orders, 39 families and 29 different genera were identified in the first voided and the midstream urine samples. Statistical differences were not identified between first voided and midstream urine samples of all bacteria groups except the Clostridiales at order level (p:0.04) and Clostridia at class level (P: .04). Conclusions: Either first voided or midstream urine samples can be used in urinary microbiota studies as we determined that there is no statistically significant difference between them regarding the results of 16s ribosomal RNA analysis. (© 2021 John Wiley & Sons Ltd.) |
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| معلومات مُعتمدة: | Türkiye Bilimsel ve Teknolojik Araştirma Kurumu |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) |
| تواريخ الأحداث: | Date Created: 20210402 Date Completed: 20210709 Latest Revision: 20210709 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/ijcp.14193 |
| PMID: | 33797158 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1742-1241 |
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| DOI: | 10.1111/ijcp.14193 |