دورية أكاديمية
Evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the E2EP3 peptide epitope of Chikungunya envelope.
العنوان: | Evaluation of neutralizing antibodies produced by papaya mosaic virus nanoparticles fused to the E2EP3 peptide epitope of Chikungunya envelope. |
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المؤلفون: | Nor Rashid N; Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia., Teoh TC; Bioinformatics Programme, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia., Al-Harbi SJ; Department of Human Anatomy, College of Medicine, University of Babylon, Iraq., Yusof R; Department of Molecular Medicine, Faculty of Medicine., Rothan HA; Department of Biology, College of Arts and Sciences, Georgia State University, Atlanta, GA, USA. |
المصدر: | Tropical biomedicine [Trop Biomed] 2021 Mar 01; Vol. 38 (1), pp. 36-41. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Malaysian Society of Parasitology and Tropical Medicine Country of Publication: Malaysia NLM ID: 8507086 Publication Model: Print Cited Medium: Internet ISSN: 2521-9855 (Electronic) Linking ISSN: 01275720 NLM ISO Abbreviation: Trop Biomed Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Kuala Lumpur : Malaysian Society of Parasitology and Tropical Medicine, [1984- |
مواضيع طبية MeSH: | Antibodies, Neutralizing/*immunology , Antibodies, Viral/*immunology , Chikungunya virus/*immunology , Viral Envelope Proteins/*immunology, Amino Acid Sequence ; Animals ; Chikungunya Fever/immunology ; Chikungunya Fever/prevention & control ; Epitopes/immunology ; Mice, Inbred ICR ; Nanoparticles ; Peptides ; Potexvirus ; Mice |
مستخلص: | Chikungunya virus (CHIKV) infection is the cause of acute symptoms and chronic symmetrical polyarthritis associated with long-term morbidity and mortality. Currently, there is no available licensed vaccine or particularly useful drug for human use against CHIKV infection. This study was conducted to evaluate the efficacy of antibodies produced by papaya mosaic virus (PapMV) nanoparticles fused to E2EP3 peptide of CHIKV envelope as a recombinant CHIKV vaccine. PapMV, PapMV-C- E2EP3, and E2EP3-N-PapMV were produced in E. coli with an approximate size of 27 to 30 kDa. ICR mice (5 to 6 weeks of age) were injected subcutaneously with 25 micrograms of vaccine construct, and ELISA measured the titer of CHIKV specific IgG antibodies. The results showed that both recombinant proteins E2EP3-N-PapMV and PapMVC-E2EP3 were able to induce IgG antibodies production in immunized mice against CHIKV while immunization with recombinant PapMV showed no IgG antibodies induction. The neutralizing activity of the antibodies generated by either E2EP3-N-PapMV or PapMV-C-E2EP3 exhibited similar inhibition to CHIKV replication in Vero cells using the cells based antibody neutralizing assay and analyzed by plaque formation assay. This study showed the effectiveness of nanoparticles vaccine generated by fusing epitope peptide of CHIKV envelope to papaya mosaic virus envelope in inducing a robust immune response in mice against CHIKV. The data showed that levels of neutralizing antibodies correlate with a protective immune response CHIKV replication. |
المشرفين على المادة: | 0 (Antibodies, Neutralizing) 0 (Antibodies, Viral) 0 (Epitopes) 0 (Peptides) 0 (Viral Envelope Proteins) |
SCR Organism: | Papaya mosaic virus |
تواريخ الأحداث: | Date Created: 20210402 Date Completed: 20211019 Latest Revision: 20240226 |
رمز التحديث: | 20240226 |
DOI: | 10.47665/tb.38.1.007 |
PMID: | 33797522 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2521-9855 |
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DOI: | 10.47665/tb.38.1.007 |