دورية أكاديمية
Making the most of high-dimensional cytometry data.
| العنوان: | Making the most of high-dimensional cytometry data. |
|---|---|
| المؤلفون: | Marsh-Wakefield FM; Vascular Immunology Unit, Discipline of Pathology, The University of Sydney, Sydney, NSW, Australia.; Charles Perkins Centre, The University of Sydney, Sydney, NSW, Australia.; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia., Mitchell AJ; Department of Chemical Engineering, Materials Characterisation and Fabrication Platform, The University of Melbourne, Parkville, VIC, Australia., Norton SE; Nanix Ltd, Dunedin, New Zealand.; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Ashhurst TM; Charles Perkins Centre, The University of Sydney, Sydney, NSW, Australia.; Sydney Cytometry, University of Sydney, Sydney, NSW, Australia.; Ramaciotti Facility for Human Systems Biology, The University of Sydney, Sydney, NSW, Australia., Leman JK; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Roberts JM; Flowjoanna Tapui Ltd, Palmerston North, New Zealand., Harte JE; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., McGuire HM; Charles Perkins Centre, The University of Sydney, Sydney, NSW, Australia.; Ramaciotti Facility for Human Systems Biology, The University of Sydney, Sydney, NSW, Australia.; Translational Immunology Group, Discipline of Pathology, The University of Sydney, Sydney, NSW, Australia., Kemp RA; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand. |
| المصدر: | Immunology and cell biology [Immunol Cell Biol] 2021 Aug; Vol. 99 (7), pp. 680-696. Date of Electronic Publication: 2021 May 04. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley Country of Publication: United States NLM ID: 8706300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1440-1711 (Electronic) Linking ISSN: 08189641 NLM ISO Abbreviation: Immunol Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2018- : [Hoboken, NJ] : Wiley Original Publication: [Adelaide, South Australia] : University of Adelaide, [c1987- |
| مواضيع طبية MeSH: | Flow Cytometry*, Humans |
| مستخلص: | High-dimensional cytometry represents an exciting new era of immunology research, enabling the discovery of new cells and prediction of patient responses to therapy. A plethora of analysis and visualization tools and programs are now available for both new and experienced users; however, the transition from low- to high-dimensional cytometry requires a change in the way users think about experimental design and data analysis. Data from high-dimensional cytometry experiments are often underutilized, because of both the size of the data and the number of possible combinations of markers, as well as to a lack of understanding of the processes required to generate meaningful data. In this article, we explain the concepts behind designing high-dimensional cytometry experiments and provide considerations for new and experienced users to design and carry out high-dimensional experiments to maximize quality data collection. (© 2021 The Authors. Immunology & Cell Biology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc.) |
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| فهرسة مساهمة: | Keywords: Analysis; experimental design; flow cytometry; high-dimensional data; mass cytometry |
| تواريخ الأحداث: | Date Created: 20210402 Date Completed: 20210928 Latest Revision: 20231107 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8453896 |
| DOI: | 10.1111/imcb.12456 |
| PMID: | 33797774 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1440-1711 |
|---|---|
| DOI: | 10.1111/imcb.12456 |