دورية أكاديمية
أعرض في EDS

The Mechanical Fingerprint of Circulating Tumor Cells (CTCs) in Breast Cancer Patients.

التفاصيل البيبلوغرافية
العنوان: The Mechanical Fingerprint of Circulating Tumor Cells (CTCs) in Breast Cancer Patients.
المؤلفون: Nel I; Department of Gynecology, Medical Center, University of Leipzig, 04103 Leipzig, Germany., Morawetz EW; Soft Matter Physics Division, Peter-Debye-Institute Leipzig, University of Leipzig, 04103 Leipzig, Germany., Tschodu D; Soft Matter Physics Division, Peter-Debye-Institute Leipzig, University of Leipzig, 04103 Leipzig, Germany., Käs JA; Soft Matter Physics Division, Peter-Debye-Institute Leipzig, University of Leipzig, 04103 Leipzig, Germany., Aktas B; Department of Gynecology, Medical Center, University of Leipzig, 04103 Leipzig, Germany.
المصدر: Cancers [Cancers (Basel)] 2021 Mar 05; Vol. 13 (5). Date of Electronic Publication: 2021 Mar 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101526829 Publication Model: Electronic Cited Medium: Print ISSN: 2072-6694 (Print) Linking ISSN: 20726694 NLM ISO Abbreviation: Cancers (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مستخلص: Circulating tumor cells (CTCs) are a potential predictive surrogate marker for disease monitoring. Due to the sparse knowledge about their phenotype and its changes during cancer progression and treatment response, CTC isolation remains challenging. Here we focused on the mechanical characterization of circulating non-hematopoietic cells from breast cancer patients to evaluate its utility for CTC detection. For proof of premise, we used healthy peripheral blood mononuclear cells (PBMCs), human MDA-MB 231 breast cancer cells and human HL-60 leukemia cells to create a CTC model system. For translational experiments CD45 negative cells-possible CTCs-were isolated from blood samples of patients with mamma carcinoma. Cells were mechanically characterized in the optical stretcher (OS). Active and passive cell mechanical data were related with physiological descriptors by a random forest (RF) classifier to identify cell type specific properties. Cancer cells were well distinguishable from PBMC in cell line tests. Analysis of clinical samples revealed that in PBMC the elliptic deformation was significantly increased compared to non-hematopoietic cells. Interestingly, non-hematopoietic cells showed significantly higher shape restoration. Based on Kelvin-Voigt modeling, the RF algorithm revealed that elliptic deformation and shape restoration were crucial parameters and that the OS discriminated non-hematopoietic cells from PBMC with an accuracy of 0.69, a sensitivity of 0.74, and specificity of 0.63. The CD45 negative cell population in the blood of breast cancer patients is mechanically distinguishable from healthy PBMC. Together with cell morphology, the mechanical fingerprint might be an appropriate tool for marker-free CTC detection.
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معلومات مُعتمدة: ERC-741350 International ERC_ European Research Council
فهرسة مساهمة: Keywords: CTCs; breast cancer; cell mechanics; circulating tumor cells; optical stretcher
تواريخ الأحداث: Date Created: 20210403 Latest Revision: 20210408
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7961579
DOI: 10.3390/cancers13051119
PMID: 33807790
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6694
DOI:10.3390/cancers13051119