دورية أكاديمية
أعرض في EDS

Saponin-adjuvanted recombinant vaccines containing rCP00660, rCP09720 or rCP01850 proteins against Corynebacterium pseudotuberculosis infection in mice.

التفاصيل البيبلوغرافية
العنوان: Saponin-adjuvanted recombinant vaccines containing rCP00660, rCP09720 or rCP01850 proteins against Corynebacterium pseudotuberculosis infection in mice.
المؤلفون: Bezerra FSB; Laboratório de Biotecnologia Infecto-parasitária, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Capão do Leão 96160-000, RS, Brazil; Laboratório de Biotecnologia Aplicada a Doenças Infecto-parasitárias, Universidade Federal Rural do Semiárido (UFERSA), Rua Francisco Mota Bairro, 572 - Pres. Costa e Silva, Mossoró, RN 59625-900, Brazil., Silva MTO; Laboratório de Biotecnologia Infecto-parasitária, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Capão do Leão 96160-000, RS, Brazil., Rezende AFS; Laboratório de Biotecnologia Infecto-parasitária, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Capão do Leão 96160-000, RS, Brazil., Lopes AS; Laboratório de Biotecnologia Infecto-parasitária, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Capão do Leão 96160-000, RS, Brazil., de Pinho RB; Laboratório de Biotecnologia Infecto-parasitária, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Capão do Leão 96160-000, RS, Brazil., Seixas FK; Laboratório de Biotecnologia Infecto-parasitária, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Capão do Leão 96160-000, RS, Brazil., Collares TV; Laboratório de Biotecnologia Infecto-parasitária, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Capão do Leão 96160-000, RS, Brazil., Portela RWD; Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia (UFBA), Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA 40110-100, Brazil., Azevedo VAC; Laboratório de Genética Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos 6627, Pampulha, Belo Horizonte, MG 31270-910, Brazil., Borsuk S; Laboratório de Biotecnologia Infecto-parasitária, Universidade Federal de Pelotas (UFPel), Campus Universitário s/n, Capão do Leão 96160-000, RS, Brazil. Electronic address: silvestre@ufersa.edu.br.
المصدر: Vaccine [Vaccine] 2021 Apr 28; Vol. 39 (18), pp. 2568-2574. Date of Electronic Publication: 2021 Apr 02.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8406899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2518 (Electronic) Linking ISSN: 0264410X NLM ISO Abbreviation: Vaccine Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam, The Netherlands : Elsevier Science
Original Publication: [Guildford, Surrey, UK] : Butterworths, [c1983-
مواضيع طبية MeSH: Corynebacterium Infections*/prevention & control , Corynebacterium pseudotuberculosis*/genetics , Saponins*, Animals ; Escherichia coli ; Mice ; Vaccines, Synthetic
مستخلص: Purpose: rCP01850, rCP09729 and rCP00660 proteins from Corynebacterium pseudotuberculosis, predicted as the three best targets to be used in vaccines against Caseous Lymphadenitis in mature epitope density (MED) analysis were tested as vaccinal targets in association to saponin as adjuvant.
Methodology: rCP00660, rCP09720 and rCP01850 were expressed in E. coli and purified for immunization assay. Balb/c mice were divided into five groups of sixteen animals each. G1 was injected with saline solution (0.9% NaCl), G2 with saponin, G3, G4 and G5 with, respectively, rCP00660, rCP09720 and rCP01850 added by saponin. Two doses were administered within a 21-days interval, and blood samples were collected for IgG quantification. Twenty-one days after the last immunization, ten mice in each group were challenged with virulent C. pseudotuberculosis MIC-6 strain, and mortality was recorded for 40 days. Meanwhile six mice in each group were used for cytokine quantification by qPCR.
Results: G2, G3, G4 and G5 presented protection rates of 10, 30, 40 and 60%, respectively. In spite of levels of total IgG were higher in G4 and G5, production of IgG2a was higher than IgG1 for G5. G3, G4 and G5 presented significant high IFN-γ levels, however, only G5 showed high TNF-α while G3 and G4 showed high IL-17.
Conclusion: rCP01850 added by saponin was able to protect efficiently mice against C. pseudotuberculosis challenge, and to induce high IgG, IFN-γ and TNF-α levels. In spite of rCP00660 and rCP09720 had not same adequate protection levels, significant IgG, IFN-γ, and IL-17 levels and further studies aiming to improve protection rates should be conducted.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: patent application for the recombinant antigens rCP01850, rCP09720 and rCP00660 in the National Institute of Industrial Property (INPI, Brazil) under the numbers BR 10 2016 016742 6, BR1020160167540, and BR1020160169380, respectively.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Caseous lymphadenitis; Immunoprophylaxis; Mature epitope density analysis; Recombinant antigens
المشرفين على المادة: 0 (Saponins)
0 (Vaccines, Synthetic)
تواريخ الأحداث: Date Created: 20210405 Date Completed: 20210524 Latest Revision: 20210524
رمز التحديث: 20231215
DOI: 10.1016/j.vaccine.2021.03.062
PMID: 33814234
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2518
DOI:10.1016/j.vaccine.2021.03.062