دورية أكاديمية
Change in gene expression levels of GABA, glutamate and neurosteroid pathways due to acoustic trauma in the cochlea.
| العنوان: | Change in gene expression levels of GABA, glutamate and neurosteroid pathways due to acoustic trauma in the cochlea. |
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| المؤلفون: | Cerrah Gunes M; Department of Medical Genetics, School of Medicine, Erciyes University, Kayseri, Turkey., Gunes MS; Department of Otolaryngology, Izmit Seka State Hospital, Kocaeli, Turkey., Vural A; Department of Otolaryngology, School of Medicine, Erciyes University, Kayseri, Turkey., Aybuga F; Center of Genome and Stem Cell, Kayseri, Turkey., Bayram A; Etlik Zübeyde Hanım Women's Diseases Education and Research Hospital, Health Sciences University, T.R. Ministry of Health, Ankara, Turkey., Bayram KK; Department of Medical Genetics, School of Medicine, Yıldirim Beyazit University, Ankara, Turkey., Sahin MI; Department of Otolaryngology, School of Medicine, Erciyes University, Kayseri, Turkey., Dogan ME; Department of Medical Genetics, School of Medicine, Erciyes University, Kayseri, Turkey., Ozdemir SY; Department of Medical Genetics, School of Medicine, Uskudar University, Istanbul, Turkey., Ozkul Y; Department of Medical Genetics, School of Medicine, Erciyes University, Kayseri, Turkey.; Center of Genome and Stem Cell, Kayseri, Turkey. |
| المصدر: | Journal of neurogenetics [J Neurogenet] 2021 Mar; Vol. 35 (1), pp. 45-57. Date of Electronic Publication: 2021 Apr 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 8406473 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1563-5260 (Electronic) Linking ISSN: 01677063 NLM ISO Abbreviation: J Neurogenet Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Informa Healthcare Original Publication: [Amsterdam : Elsevier Science Publishers, c1983- |
| مواضيع طبية MeSH: | Cochlea/*metabolism , Glutamic Acid/*genetics , Hearing Loss, Noise-Induced/*genetics , Neurosteroids/*metabolism , gamma-Aminobutyric Acid/*genetics, Animals ; Glutamic Acid/metabolism ; Hair Cells, Auditory, Inner/metabolism ; Hair Cells, Auditory, Outer/metabolism ; Hearing Loss, Noise-Induced/metabolism ; Mice ; Mice, Inbred BALB C ; gamma-Aminobutyric Acid/metabolism |
| مستخلص: | The characteristic feature of noise-induced hearing loss (NIHL) is the loss or malfunction of the outer hair cells (OHC) and the inner hair cells (IHC) of the cochlea. 90-95% of the spiral ganglion neurons, forming the cell bodies of cochlear nerve, synapse with the IHCs. Glutamate is the most potent excitatory neurotransmitter for IHC-auditory nerve synapses. Excessive release of glutamate in response to acoustic trauma (AT), may cause excitotoxicity by causing damage to the spiral ganglion neurons (SGN) or loss of the spiral ganglion dendrites, post-synaptic to the IHCs. Another neurotransmitter, GABA, plays an important role in the processing of acoustic stimuli and central regulation after peripheral injury, so it is potentially related to the regulation of hearing function and sensitivity after noise. The aim of this study is to evaluate the effect of AT on the expressions of glutamate excitotoxicity, GABA inhibition and neurosteroid synthesis genes.We exposed 24 BALB/c mice to AT. Controls were sacrificed without exposure to noise, Post-AT(1) and Post-AT(15) were sacrificed on the 1st and 15th day, respectively, after noise exposure. The expressions of various genes playing roles in glutamate, GABA and neurosteroid pathways were compared between groups by real-time PCR.Expressions of Cyp11a1 , Gls , Gabra1 , Grin2b , Sult1a1 , Gad1, and Slc1a2 genes in Post-AT(15) mice were significantly decreased in comparison to control and Post-AT(1) mice. No significant differences in the expression of Slc6a1 and Slc17a8 genes was detected.These findings support the possible role of balance between glutamate excitotoxicity and GABA inhibition is disturbed during the post AT days and also the synthesis of some neurosteroids such as pregnenolone sulfate may be important in this balance. |
| فهرسة مساهمة: | Keywords: Acoustic trauma; GABA; gene expression; glutamate; neurosteroid |
| المشرفين على المادة: | 0 (Neurosteroids) 3KX376GY7L (Glutamic Acid) 56-12-2 (gamma-Aminobutyric Acid) |
| تواريخ الأحداث: | Date Created: 20210407 Date Completed: 20220110 Latest Revision: 20220110 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1080/01677063.2021.1904922 |
| PMID: | 33825593 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1563-5260 |
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| DOI: | 10.1080/01677063.2021.1904922 |