دورية أكاديمية
Transcriptome-Wide Prediction and Measurement of Combined Effects Induced by Chemical Mixture Exposure in Zebrafish Embryos.
العنوان: | Transcriptome-Wide Prediction and Measurement of Combined Effects Induced by Chemical Mixture Exposure in Zebrafish Embryos. |
---|---|
المؤلفون: | Schüttler A; Department Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany.; Institute for Environmental Research, Rheinisch-Westfälische Technische Hochschule Aachen, Aachen, Germany., Jakobs G; Department Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany., Fix JM; Department Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany., Krauss M; Department Effect-Directed Analysis, UFZ, Leipzig, Germany., Krüger J; Department Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany., Leuthold D; Department Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany., Altenburger R; Department Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany.; Institute for Environmental Research, Rheinisch-Westfälische Technische Hochschule Aachen, Aachen, Germany., Busch W; Department Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany. |
المصدر: | Environmental health perspectives [Environ Health Perspect] 2021 Apr; Vol. 129 (4), pp. 47006. Date of Electronic Publication: 2021 Apr 07. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: National Institute of Environmental Health Sciences Country of Publication: United States NLM ID: 0330411 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-9924 (Electronic) Linking ISSN: 00916765 NLM ISO Abbreviation: Environ Health Perspect Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Research Triangle Park, N. C. National Institute of Environmental Health Sciences. |
مواضيع طبية MeSH: | Water Pollutants, Chemical* , Zebrafish*, Animals ; Environmental Exposure ; Humans ; Toxicogenetics ; Transcriptome |
مستخلص: | Background: Humans and environmental organisms are constantly exposed to complex mixtures of chemicals. Extending our knowledge about the combined effects of chemicals is thus essential for assessing the potential consequences of these exposures. In this context, comprehensive molecular readouts as retrieved by omics techniques are advancing our understanding of the diversity of effects upon chemical exposure. This is especially true for effects induced by chemical concentrations that do not instantaneously lead to mortality, as is commonly the case for environmental exposures. However, omics profiles induced by chemical exposures have rarely been systematically considered in mixture contexts. Objectives: In this study, we aimed to investigate the predictability of chemical mixture effects on the whole-transcriptome scale. Methods: We predicted and measured the toxicogenomic effects of a synthetic mixture on zebrafish embryos. The mixture contained the compounds diuron, diclofenac, and naproxen. To predict concentration- and time-resolved whole-transcriptome responses to the mixture exposure, we adopted the mixture concept of concentration addition. Predictions were based on the transcriptome profiles obtained for the individual mixture components in a previous study. Finally, concentration- and time-resolved mixture exposures and subsequent toxicogenomic measurements were performed and the results were compared with the predictions. Results: This comparison of the predictions with the observations showed that the concept of concentration addition provided reasonable estimates for the effects induced by the mixture exposure on the whole transcriptome. Although nonadditive effects were observed only occasionally, combined, that is, multicomponent-driven, effects were found for mixture components with anticipated similar, as well as dissimilar, modes of action. Discussion: Overall, this study demonstrates that using a concentration- and time-resolved approach, the occurrence and size of combined effects of chemicals may be predicted at the whole-transcriptome scale. This allows improving effect assessment of mixture exposures on the molecular scale that might not only be of relevance in terms of risk assessment but also for pharmacological applications. https://doi.org/10.1289/EHP7773. |
التعليقات: | Comment in: Environ Health Perspect. 2021 Oct;129(10):104001. (PMID: 34609158) |
References: | Environ Toxicol Chem. 2016 Mar;35(3):513-25. (PMID: 26923854) PLoS One. 2014 May 02;9(5):e96580. (PMID: 24794244) Environ Toxicol Chem. 2016 Jan;35(1):20-35. (PMID: 26771350) Environ Health Perspect. 2019 Apr;127(4):47002. (PMID: 30964323) Environ Toxicol Chem. 2008 Mar;27(3):509-18. (PMID: 17983273) J Hazard Mater. 2017 Jul 15;334:121-131. (PMID: 28407539) Crit Rev Toxicol. 2018 Oct;48(9):796-814. (PMID: 30632445) Environ Sci Pollut Res Int. 2008 Jul;15(5):394-404. (PMID: 18575912) Environ Health Perspect. 2007 Dec;115 Suppl 1:106-14. (PMID: 18174958) Bioinformatics. 2003 Jan 22;19(2):185-93. (PMID: 12538238) Environ Int. 2018 May;114:95-106. (PMID: 29499452) Carcinogenesis. 2015 Jun;36 Suppl 1:S254-96. (PMID: 26106142) PLoS One. 2014 Feb 12;9(2):e88808. (PMID: 24533151) Crit Rev Toxicol. 2019 Feb;49(2):174-189. (PMID: 30931677) J Theor Biol. 1985 Jun 7;114(3):413-31. (PMID: 4021503) EFSA J. 2019 Mar 25;17(3):e05634. (PMID: 32626259) Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4416-E4425. (PMID: 29686083) Toxicol Sci. 2007 Jul;98(1):240-8. (PMID: 17449896) Environ Sci Technol. 2019 Aug 6;53(15):8611-8620. (PMID: 31287672) Science. 2020 Jan 24;367(6476):388-392. (PMID: 31974244) Adv Cancer Res. 1981;35:269-335. (PMID: 7041539) Environ Sci Technol. 2004 Dec 1;38(23):6353-62. (PMID: 15597892) Regul Toxicol Pharmacol. 2011 Apr 2;:. (PMID: 21466831) Sci Total Environ. 2016 May 15;553:671-672. (PMID: 26972864) Environ Toxicol Chem. 2016 Aug;35(8):1887-99. (PMID: 27299692) Environ Toxicol Chem. 2001 Feb;20(2):448-57. (PMID: 11351447) Elife. 2020 Sep 18;9:. (PMID: 32945258) Nucleic Acids Res. 2021 Jan 8;49(D1):D1138-D1143. (PMID: 33068428) Cell Syst. 2016 Jul;3(1):35-42. (PMID: 27453445) Cell. 2018 Sep 20;175(1):277-291.e31. (PMID: 30241608) Environ Sci Technol. 2017 May 02;51(9):4792-4802. (PMID: 28401767) Integr Environ Assess Manag. 2007 Jul;3(3):364-72. (PMID: 17695109) Environ Toxicol Chem. 2015 Sep;34(9):2167-80. (PMID: 25900799) Neural Netw. 2002 Oct-Nov;15(8-9):953-66. (PMID: 12416686) Ecotoxicology. 2002 Oct;11(5):299-310. (PMID: 12463676) Sci Adv. 2016 Sep 07;2(9):e1601272. (PMID: 27617294) Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):10442-7. (PMID: 27562164) Toxins (Basel). 2019 Mar 19;11(3):. (PMID: 30893800) Gigascience. 2019 Jun 1;8(6):. (PMID: 31140561) Environ Sci Technol. 2002 Apr 15;36(8):1751-6. (PMID: 11993873) Environ Health Perspect. 2007 Dec;115 Suppl 1:122-8. (PMID: 18174960) Environ Int. 2020 Jan;134:105267. (PMID: 31704565) Nat Commun. 2019 Jun 17;10(1):2674. (PMID: 31209238) Dev Dyn. 1995 Jul;203(3):253-310. (PMID: 8589427) Toxicol Sci. 2017 Mar 1;156(1):133-148. (PMID: 28069987) Toxicol Sci. 2010 Nov;118(1):286-97. (PMID: 20702594) PLoS One. 2014 Sep 04;9(9):e106523. (PMID: 25187966) Aquat Toxicol. 2001 Dec 3;56(1):13-32. (PMID: 11690628) Environ Sci Technol. 2012 Mar 6;46(5):2508-22. (PMID: 22283441) Pharmacol Rev. 1995 Jun;47(2):331-85. (PMID: 7568331) Toxicol Sci. 2017 Jun 1;157(2):291-304. (PMID: 28329862) Environ Toxicol Chem. 2018 Apr;37(4):955-969. (PMID: 29350428) Environ Toxicol Chem. 2013 Aug;32(8):1685-7. (PMID: 23843317) Nat Chem Biol. 2006 Sep;2(9):458-66. (PMID: 16921358) BMC Genomics. 2008;9 Suppl 1:S15. (PMID: 18366604) Arch Toxicol. 2017 Jul;91(7):2599-2616. (PMID: 27858113) |
المشرفين على المادة: | 0 (Water Pollutants, Chemical) |
تواريخ الأحداث: | Date Created: 20210407 Date Completed: 20211125 Latest Revision: 20220314 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC8041271 |
DOI: | 10.1289/EHP7773 |
PMID: | 33826412 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1552-9924 |
---|---|
DOI: | 10.1289/EHP7773 |