دورية أكاديمية
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Frequency and characterization of RHD variant alleles in a population of blood donors from southeastern Brazil: Comparison with other populations.

التفاصيل البيبلوغرافية
العنوان: Frequency and characterization of RHD variant alleles in a population of blood donors from southeastern Brazil: Comparison with other populations.
المؤلفون: Rodrigues ES; Center for Cell-Based Research, Regional Blood Center of Ribeirão Preto, School of Medicine of Ribeirão Preto, São Paulo, Brazil; University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil. Electronic address: evandra@hemocentro.fmrp.usp.br., Romagnoli AC; Center for Cell-Based Research, Regional Blood Center of Ribeirão Preto, School of Medicine of Ribeirão Preto, São Paulo, Brazil; University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Santos FLS; Center for Cell-Based Research, Regional Blood Center of Ribeirão Preto, School of Medicine of Ribeirão Preto, São Paulo, Brazil; University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Cutter TB; Center for Cell-Based Research, Regional Blood Center of Ribeirão Preto, School of Medicine of Ribeirão Preto, São Paulo, Brazil; University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Catelli LF; Center for Cell-Based Research, Regional Blood Center of Ribeirão Preto, School of Medicine of Ribeirão Preto, São Paulo, Brazil; University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Cédric V; Département Centre National de Référence pour les Groupes Sanguins, Institut National de la Transfusion Sanguine (INTS), Paris, France., Peyrard T; Département Centre National de Référence pour les Groupes Sanguins, Institut National de la Transfusion Sanguine (INTS), Paris, France., Covas DT; Center for Cell-Based Research, Regional Blood Center of Ribeirão Preto, School of Medicine of Ribeirão Preto, São Paulo, Brazil; University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil; Department of Clinical Medicine, School of Medicine of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., de Castilho LM; Laboratório de Imunohematologia Molecular do Hemocentro - Unicamp, Campinas, SP, Brazil., Kashima S; Center for Cell-Based Research, Regional Blood Center of Ribeirão Preto, School of Medicine of Ribeirão Preto, São Paulo, Brazil; University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
المصدر: Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis [Transfus Apher Sci] 2021 Aug; Vol. 60 (4), pp. 103135. Date of Electronic Publication: 2021 Apr 08.
نوع المنشور: Clinical Trial; Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Country of Publication: England NLM ID: 101095653 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1473-0502 (Print) Linking ISSN: 14730502 NLM ISO Abbreviation: Transfus Apher Sci
أسماء مطبوعة: Original Publication: Oxford : Pergamon : Elsevier Science, c2001-
مواضيع طبية MeSH: Alleles* , Blood Donors* , Polymorphism, Single-Stranded Conformational*, Rh-Hr Blood-Group System/*genetics, Brazil ; Female ; Humans ; Male ; Multiplex Polymerase Chain Reaction
مستخلص: Background: The correct determination of D antigen could help to avoid alloimmunization in pregnant women and patients receiving blood transfusions. However, there are limitations in the identification of D variants as the partial and weak D phenotypes make the determination of D antigen a great challenge in the transfusion routine.'
Study Design and Methods: The molecular characterization of D variants was performed on blood donors from southeastern Brazil with atypical D typing. Furthermore, the serological profile of all RHD variant alleles identified was analyzed using different Anti-D clones. The prevalence of RHD alleles and genotypes found was compared with those described in other countries and in other regions from Brazil.
Results: Atypical serologic D typing occurred in 0.79 % of blood donors. The majority of RHD variant alleles (88 %) were first characterized by multiplex PCR and PCR-SSP as RHD*weak partial 4 (47 %), followed by RHD*weak D type 3 (29.9 %), RHD*weak D type 2 (3.9 %) and RHD*weak D type 1 (3.1 %). Genomic DNA sequencing characterized the RHD*weak partial 4 variants found in RHD*DAR1.2 (weak 4.2.2) (22 %), RHD*DAR3 (weak 4.0.1) (2.4 %), RHD*DAR3.1 (weak 4.0) (22 %) and RHD*DAR4 (weak 4.1) (0.8 %). RHD variant alleles associated with partial D, such as, RHD*DAU-4 (1.6 %), RHD*DAU-5 (2.4 %), RHD*DAU-6 (1.6 %), RHD* DIII type 8 (1.6 %), RHD*DVII (3.9 %) and RHD* DMH (0.8 %) were also observed.
Conclusion: The prevalence of RHD variant alleles observed in this cohort differ from those found in other populations, including Brazilians from other regions. RHD allele distribution in specific regions should be considered for implementation of algorithms and genotyping strategies aiming at a more effective and safe transfusion.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: RHD allele distribution; RHD variants; RHD* partial D; RHD*weak D
المشرفين على المادة: 0 (Rh-Hr Blood-Group System)
0 (Rho(D) antigen)
تواريخ الأحداث: Date Created: 20210419 Date Completed: 20220112 Latest Revision: 20220112
رمز التحديث: 20221213
DOI: 10.1016/j.transci.2021.103135
PMID: 33867285
قاعدة البيانات: MEDLINE
الوصف
تدمد:1473-0502
DOI:10.1016/j.transci.2021.103135