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Desulfovibrio diazotrophicus sp. nov., a sulfate-reducing bacterium from the human gut capable of nitrogen fixation.

التفاصيل البيبلوغرافية
العنوان: Desulfovibrio diazotrophicus sp. nov., a sulfate-reducing bacterium from the human gut capable of nitrogen fixation.
المؤلفون: Sayavedra L; Gut Health and Microbes, Quadram Institute Bioscience, Norwich Research Park, Norwich, UK., Li T; Gut Health and Microbes, Quadram Institute Bioscience, Norwich Research Park, Norwich, UK.; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China., Bueno Batista M; Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, UK., Seah BKB; Max Planck Institute for Developmental Biology, Tübingen, Germany., Booth C; Gut Health and Microbes, Quadram Institute Bioscience, Norwich Research Park, Norwich, UK., Zhai Q; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China., Chen W; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China., Narbad A; Gut Health and Microbes, Quadram Institute Bioscience, Norwich Research Park, Norwich, UK.
المصدر: Environmental microbiology [Environ Microbiol] 2021 Jun; Vol. 23 (6), pp. 3164-3181. Date of Electronic Publication: 2021 May 14.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Blackwell Science Country of Publication: England NLM ID: 100883692 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1462-2920 (Electronic) Linking ISSN: 14622912 NLM ISO Abbreviation: Environ Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford : Blackwell Science, 1999-
مواضيع طبية MeSH: Desulfovibrio*/genetics , Desulfovibrio*/metabolism , Nitrogen Fixation*, Animals ; Bacteria/metabolism ; Humans ; Nitrogenase/metabolism ; Oxidation-Reduction ; Phylogeny ; Sulfates
مستخلص: Sulfate-reducing bacteria (SRB) are widespread in human guts, yet their expansion has been linked to colonic diseases. We report the isolation, sequencing and physiological characterization of strain QI0027 T , a novel SRB species belonging to the class Desulfovibrionia. Metagenomic sequencing of stool samples from 45 Chinese individuals, and comparison with 1690 Desulfovibrionaceae metagenome-assembled genomes recovered from humans of diverse geographic locations, revealed the presence of QI0027 T in 22 further individuals. QI0027 T encoded nitrogen fixation genes and based on the acetylene reduction assay, actively fixed nitrogen. Transcriptomics revealed that QI0027 T overexpressed 42 genes in nitrogen-limiting conditions compared to cultures supplemented with ammonia, including genes encoding nitrogenases, a urea uptake system and the urease complex. Reanalyses of 835 public stool metatranscriptomes showed that nitrogenase genes from Desulfovibrio bacteria were expressed in six samples suggesting that nitrogen fixation might be active in the gut environment. Although frequently thought of as a nutrient-rich environment, nitrogen fixation can occur in the human gut. Animals are often nitrogen limited and have evolved diverse strategies to capture biologically active nitrogen, ranging from amino acid transporters to stable associations with beneficial microbes that provide fixed nitrogen. QI0027 T is the first Desulfovibrio human isolate for which nitrogen fixation has been demonstrated, suggesting that some sulfate-reducing bacteria could also play a role in the availability of nitrogen in the gut.
(© 2021 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)
التعليقات: Erratum in: Environ Microbiol. 2022 Oct;24(10):4971. (PMID: 36254869)
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معلومات مُعتمدة: BBS/E/F/00044453 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/R012512/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BBS/E/F/000PR10356 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/R012490/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/N003608/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BBS/E/F/00042241 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BBS/E/F/000PR10353 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/N013476/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BBS/E/F/000PR10355 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BBS/E/F/000PR10346 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BBS/OS/NW/000006 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
المشرفين على المادة: 0 (Sulfates)
EC 1.18.6.1 (Nitrogenase)
تواريخ الأحداث: Date Created: 20210420 Date Completed: 20211015 Latest Revision: 20221018
رمز التحديث: 20240628
DOI: 10.1111/1462-2920.15538
PMID: 33876566
قاعدة البيانات: MEDLINE
الوصف
تدمد:1462-2920
DOI:10.1111/1462-2920.15538