دورية أكاديمية
A novel acrylic resin palatal device contaminated with Candida albicans biofilm for denture stomatitis induction in Wistar rats.
| العنوان: | A novel acrylic resin palatal device contaminated with Candida albicans biofilm for denture stomatitis induction in Wistar rats. |
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| المؤلفون: | Moraes GS; Universidade Estadual de Ponta Grossa, Departamento de Odontologia, Ponta Grossa, Paraná, Brasil., Albach T; Universidade Estadual de Ponta Grossa, Departamento de Odontologia, Ponta Grossa, Paraná, Brasil., Ramos IE; Universidade Estadual de Ponta Grossa, Departamento de Odontologia, Ponta Grossa, Paraná, Brasil., Kopacheski MG; Universidade Estadual de Ponta Grossa, Departamento de Odontologia, Ponta Grossa, Paraná, Brasil., Cachoeira VS; Universidade Estadual de Ponta Grossa, Departamento de Odontologia, Ponta Grossa, Paraná, Brasil., Sugio CYC; Universidade de São Paulo, Faculdade de Odontologia de Bauru, Bauru, São Paulo, Brasil., Galvão Arrais CA; Universidade Estadual de Ponta Grossa, Departamento de Odontologia, Ponta Grossa, Paraná, Brasil., Neppelenbroek KH; Universidade de São Paulo, Faculdade de Odontologia de Bauru, Bauru, São Paulo, Brasil., Urban VM; Universidade Estadual de Ponta Grossa, Departamento de Odontologia, Ponta Grossa, Paraná, Brasil. |
| المصدر: | Journal of applied oral science : revista FOB [J Appl Oral Sci] 2021 Apr 19; Vol. 29, pp. e20200865. Date of Electronic Publication: 2021 Apr 19 (Print Publication: 2021). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Faculdade de Odontologia de Bauru, USP Country of Publication: Brazil NLM ID: 101189774 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-7765 (Electronic) Linking ISSN: 16787757 NLM ISO Abbreviation: J Appl Oral Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bauru, SP : Faculdade de Odontologia de Bauru, USP, [2003]- |
| مواضيع طبية MeSH: | Candida albicans* , Stomatitis, Denture*, Acrylic Resins ; Animals ; Biofilms ; Denture Bases ; Palate ; Rats ; Rats, Wistar |
| مستخلص: | Denture stomatitis is the most frequent oral lesion in removable prosthesis wearers, with high recurrence rates and a complex treatment. Objective: This study describes a protocol to obtain and to contaminate a palatal device with Candida albicans biofilm that could be used for an animal model of denture stomatitis. Methodology: Acrylic resin devices (N=41) were obtained from impressions of the palates of Wistar rats with individual trays and polyether. The efficacy of microwave irradiation (MW), ultraviolet light (UV), or ultrasonic bath (US) was assessed by colony viability and spectrophotometric analyses (n=5) in order to select the most appropriate method for sterilizing the devices. Then, different devices (n=5) were contaminated with C. albicans and evaluated by CFU/mL determination, scanning electron microscopy, and laser confocal microscopy. Device stabilization was assessed with either autopolymerizing acrylic resins or a self-adhesive resin cement (n=2). The spectrophotometric data were analyzed by one-way ANOVA followed by the Tukey's HSD post-hoc test (α=0.05). Results: MW was the only method capable of sterilizing the devices, and the contamination protocol developed a mature and viable C. albicans biofilm (~1.2 x 106 CFU/mL). The self-adhesive resin cement was the best stabilization material. Conclusions: This acrylic resin palatal device was designed to be similar to the clinical situation of contaminated prostheses, with easy manufacturing and handling, effective stabilization, and satisfactory contamination. Thus, the acrylic device can be a valuable tool in the development of denture stomatitis in rats. |
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| المشرفين على المادة: | 0 (Acrylic Resins) |
| تواريخ الأحداث: | Date Created: 20210422 Date Completed: 20210426 Latest Revision: 20210502 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8075290 |
| DOI: | 10.1590/1678-7757-2020-0865 |
| PMID: | 33886943 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1678-7765 |
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| DOI: | 10.1590/1678-7757-2020-0865 |